The effects of plant growth regulator on male gonads and fertility – toxicity study on pigs

Project Details


Possible effects of the plant growth regulator chlormequat on male reproduction in the pig are being investigated. Pig was chosen as a model for humans, since it was anticipated that this species was closer to the human with regard to sensitivity to chlormequat than conventional laboratory animals. The investigation was performed on male reproduction traits, because male animals are more sensitive to environmental disrupters than female animals and are thus preferred in order to reduce the risk of a false negative result.

Hypothesis: consumption of chlormequat equal to the ADI-value will compromise reproduction traits compared to no consumption of chlormequat.

The experiment was conducted as an oral toxicity study in pig. Three groups of sows were fed a diet with or without chlormequat, respectively, during pregnancy and subsequent lactation. For the two groups who were given chlormequat spiked feed, the levels were equivalent to ADI or ½ ADI (ADI = 0.05 mg per kg body weight per day).

The concentration of chlormequat in the feed was controlled by an accredited method based on LC/MS/MS. Piglets from each sow were sacrificed immediately after birth, at 4 weeks and at 4 months of age to assess the various effects of chlormequat. In order to investigate a possible transfer of chlormequat from mother to offspring, samples of mothers milk and blood were collected and analysed for chlormequat. Additionally, residues of chlormequat in human milk and placenta were performed.
Effective start/end date01/01/200431/12/2006