The E. coli GFP gene.

    Project Details


    The GFP protein found in the yellyfish Aequorea fluoresce in UV light. This protein has been widely used in several laboratories during the last 4-5 years because of its spectral properties. The protein alone or fused to other protein has been used to record a wide variety of biological activities and/or localization of biological structures in the living cell. Other laboratories have described altered derivatives of the GFP protein with changed emission and/or excitation spectra. However, there are problems with the GFP protein and its altered derivatives in procaryotic cells. Therefore, we are in the process of constructing GFP genes with optimal expression of GFP protein in procaryotic cells. At present we have constructed a synthetic GFP gene with the translational codon usage characteristic of procaryotic (E. coli) cells. The level of GFP expression (fluorescence) from cells carrying this synthetic gene is very high and has prompted us to try to construct even better derivatives. The purpose is 1.) to study the cellular localization of a number of important proteins interacting with the bacterial chromosome, and 2.) to use the gene as a tool to study cell cycle regulated gene expression.
    During 1998 we have constructed a number of proteins with codon optimized GFP fused to DNA binding proteins.
    Effective start/end date01/02/199701/01/9999