Phage-host interactions: The integration system of the lactococcal phage TP901-1

  • Hammer, Karin (Project Manager)
  • Breuner, Anne (Project Participant)
  • Sørensen, Lise (Project Participant)

    Project Details

    Description

    The temperate phage TP901-1 is able to integrate its genome at one particular locus (attB) on the lactococcal chromosome, when entering the lysogenic life cycle. The presence of the phage genome as a so-called prophage on the chromosome ensures replication of the phage genome and thus the inheritance of the phage genome to the bacterial daughter cells during growth. Induction of the prophage, which occurs occasionally or may be induced by DNA damaging agents, leads to release of phage particles and cell lysis. This process requires specific exception of the phage genome from the bacterial chromosome.
    The aim of this project is a molecular analysis of the site specific recombination processes of TP901-1, with particular emphasis on the defining the role of the bacterial attB site for the integration process and on defining the phage encoded proteins involved in the exception process.
    Collaboration with the EU funded project on Site specific recombination occurs as planned.
    Excision vectors have been constructed based on the upp gene from the pyrimidine salvage pathway in lactococcus lactis. They are useful tools for any project investigating excision events. Several kb's of the lactococcal chromosome surrounding the attB site have been sequenced.
    Also vectors for defining the functional size of the attB site and for isolation of mutants in this site have been constructed.
    StatusFinished
    Effective start/end date01/03/199528/02/1998

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