Micro fabricated flow system for manipulation and sorting of living cells

  • Blankenstein, Gert (Project Manager)
  • Larsen, Ulrik Darling (Project Participant)
  • Branebjerg, Jens (Project Participant)

    Project Details

    Description

    A novel micro machined system for manipulation, analysis and sorting of particles and living cells has been designed. The micro tool was developed for imaging cytometry allowing both micro liquid handling (sample pre treatment: filtration, reagent addition and rapid mixing) and optical detection. Micro channels (200 mm x 50 mm) are etched in a silicon wafer and covered with a boron glass plate, which allows the external observation and detection of the sample inside the micro channels using a microscope system. Due to the small channel dimensions (500 mm x 100 mm, 200 mm x 50 mm) the flow is laminar with negligible influence of inertia forces. With the design of a totally closed system laboratory contamination is avoided.
    Cells and particles were detected by fluorescence using a CCD camera and a PMT system. Additionally electrical counting and characterisation of the sample were performed inside the micro system using the Coulter principle. In this study we describe results of fluorescence and "coulter" measurements on fluorescent-labelled particles and cells in suspension (T-lymphocytes). Examples of cell sorting will be presented to illustrate the potential of micro fluid systems in cellular analysis. By using a fluidic separation method sorting frequencies up to 2,000 events/sec can be realised. The novel sorting method allows an enrichment of rare cells with negligible dilution of the sorted cells. The presented micro flow device is a versatile tool for cell manipulation: Besides rapid cell sorting, kinetic assays or stimulation studies can be performed. The perspectives of micro fabricated instrumentation in analytical cytology and medicine are discussed.
    StatusActive
    Effective start/end date01/01/1996 → …