In this project we study the control and regulation of free-energy metabolism in the bacterium Lactococcus lactis. This is a relatively simple model system, because these cells only make ATP through substrate level phosphorylation. Experimentally, we modulate the expression of the genes encoding the H+-ATPase and the genes encoding the enzymes in and surrounding the glycolytic pathway. In order to understand, in a quantitative way, the control and regulation in this system, we use biomathematical tools such as metabolic control analysis and computer modelling.
|Effective start/end date||01/03/1998 → 01/01/9999|