Control of Metabolic flux through glycolysis in L. lactis

  • Hammer, Karin (Project Manager)
  • Willemoes, Martin (Project Participant)
  • Andersen, Heidi Winterberg (Project Participant)
  • Madsen, Katrine (Project Participant)
  • Jensen, Peter Ruhdal (Project Participant)
  • Jochumsen, Kirsten Væver (Project Participant)
  • Villadsen, John (Project Participant)
  • Hansen, Kianoush Kangarlou (Project Participant)

    Project Details

    Description

    This project is a collaboration between the Department of Microbiology (DTU), Department of Biotechnology (DTU) and The danish dairy research foundation (MFF).
    The project consists of three subprojects as listed below. The aim of the overall project is to describe the control by individual glycolytic enzymes on the flux through glycolysis in L. lactis. The approach is: 1) to study the concentration of glycolytic intermediates in chemostat experiments and design a mathematical model that describes the observations , 2) to modulate the expression of genes encoding glycolytic enzymes around their wildtype level and study the effect on glycolytic flux, the level of glycolytic intermediate levels and endproducts, 3) to introduce mutations in the genes of interest and study the effect on glycolytic flux, intermediates and endproducts.
    The three subprojects have the following titles:
    A. Mathematic Modelling of glycolysis in Lactococcus lactis.
    B. Metabolic Control Analysis of glycolysis in Lactococcus lactis
    C. Isolation and characterization of glycolytic mutants
    StatusFinished
    Effective start/end date01/02/199631/08/1999

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