Control and Regulation of DNA supercoiling in E. coli

This project is a collaboration with professor Westerhoffs and Dr. Snoeps group at the Free University, Amsterdam, The Netherlands. Using genetic engineering, we have contructed E. coli mutants that allow us to modulate the expression of the genes involved in DNA supercoiling, i.e. the genes encoding DNA gyrase and topoisomerase I and to determine the control by these enzymes on DNA supercoiling and expression of various genes in E. coli