Biochemical aspects of intestinal iron absorption.

  • Bukhave, Klaus (Project Manager)
  • Søndergaard, Ib (Project Participant)
  • Sørensen, Anne Dorthe (Project Participant)

    Project Details


    This study is a PhD-project for Anne Dorthe Sørensen with Klaus Bukhave and Ib Sønderghaard as supervisors. It represents a sub-project of "Improved iron status through utilisation of meat proteins for an improved food iron availability and through safe food fortification", a co-operational project by groups from KVL and DTU.
    The intestinal absorption of iron is studied in model systems, including isolated enterocytes, the artificial cell line, Caco-2, intact sheets of jejunal mucosa mounted either in classical "Ussing-chambers" or in special constructed "influx-chambers", and "tied-off" loops of rat jejunum, in vivo.
    Isolation, purification, and characterization of the putative "meat factor" claimed to facilitate the absorption of non-heme iron is based on separation and computer techniques developed in our laboratory. These analytical methods include electrophoretic techniques (1-D, 2-D, capillary, isoelectric focusing), image processing, HPLC and FPLC chromatography, and Matrix-assisted laser desorption/ionization - time of flight (MALDI-TOF) mass spectrometry.
    Effective start/end date15/09/199814/03/2001


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