Absorption and metabolisme of lipid oxidation products in the rat small intestine

  • Bukhave, Klaus (Project Manager)

    Project Details


    This study is a PhD-project for MSc Lone Skoubo Berthelsen, with Klaus Bukhave and Gunhild Hølmer as supervisors.
    The absorption and metabolism of lipid oxidation products are studied in model systems, including isolated enterocytes, artificial cell lines (Caco-2, T84), intact sheets of jejunal mucosa mounted in classical "Ussing-chambers", and "tied-off" loops of rat jejunum, in vivo. For these purposes, radioactive labelled compounds, like 1-14C-linoleic acid hydroperoxide, 1-3H-2-trans-nonenal, and 5,6-3H-4-hydroxy-2-trans-nonenal are synthetized and charactherized. HPLC- and GC-MS systems are developed for separation of the above mentioned compounds and their metabolites. In addition, them effects of lipid oxidation products on cellular oxidation status as well as their implications on signal-transduction mechanisms are investigated.
    Effective start/end date01/04/199531/08/1998