A genetic and a molecular biological system for Phaffia rhodozyma

  • Villadsen, Ingrid Stampe (Project Manager)
  • Langkjær, Rikke Breinhold (Project Participant)

    Project Details


    The basidiomycetous yeast Phaffia rhodozyma produce astaxanthin, a carotenoid pigment, that is the strongest antioxidant known so far. It must be fed to crusteaceans and salmonoids in order for them to obtain their red color. The goal is to improve the production of astaxanthin from this yeast using molecular biological methods.
    A transformation system has been developed, but only few genes have been isolated. More plasmids are required as well as the isolation of mutants. The existence of a sexual cycle will ease further work with isolated mutants.
    Out of these required parameters, the identification of the conditions that allows a full sexual cycle have been completed. Also several different mutants have been isolated. Finally, cDNA clones representing three extracellular enzymes and two intracellular enzymes have been isolated and sequenced.
    Effective start/end date01/01/1995 → …