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Research Output 2012 2019

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Journal article
2019
21 Downloads (Pure)

Industrializing a Bacterial Strain for l -Serine Production through Translation Initiation Optimization

Rennig, M., Mundhada, H., Wordofa, G. G., Gerngross, D., Wulff, T., Worberg, A., Nielsen, A. T. & Nørholm, M. H. H., 2019, In : ACS Synthetic Biology. 8, 10, p. 2347-2358

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
198 Downloads (Pure)

Meta synthetic biology: controlling the evolution of engineered living systems

Nørholm, M. H. H., 2019, In : Microbial Biotechnology. 12, 1, p. 35-37

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
80 Downloads (Pure)

Mutations in the Global Transcription Factor CRP/CAP: Insights from Experimental Evolution and Deep Sequencing

Frendorf, P. O., Lauritsen, I., Sekowska, A., Danchin, A. & Nørholm, M. H. H., 2019, In : Computational and Structural Biotechnology Journal. 17, p. 730-736

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
2018
292 Downloads (Pure)

A synbio approach for selection of highly expressed gene variants in Gram-positive bacteria

Ferro, R., Rennig, M., Hernández Rollán, C., Daley, D. O. & Nørholm, M. H. H., 2018, In : Microbial Cell Factories. 17, 1, 11 p., 37.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
241 Downloads (Pure)

BacHBerry:: BACterial Hosts for production of Bioactive phenolics from bERRY fruits

Dudnik, A., Almeida, A. F., Andrade, R., Avila, B., Bañados, P., Barbay, D., Bassard, J. E., Benkoulouche, M., Bott, M., Braga, A., Breitel, D., Brennan, R., Bulteau, L., Chanforan, C., Costa, I., Costa, R. S., Doostmohammadi, M., Faria, N., Feng, C., Fernandes, A. & 63 others, Ferreira, P., Ferro, R., Foito, A., Freitag, S., Garcia, G., Gaspar, P., Godinho-Pereira, J., Hamberger, B., Hartmann, A., Heider, H., Jardim, C., Julien-Laferriere, A., Kallscheuer, N., Kerbe, W., Kuipers, O. P., Li, S., Love, N., Marchetti-Spaccamela, A., Marienhagen, J., Martin, C., Mary, A., Mazurek, V., Meinhart, C., Sevillano, D. M., Menezes, R., Naesby, M., Nørholm, M. H. H., Okkels, F. T., Oliveira, J., Ottens, M., Parrot, D., Pei, L., Rocha, I., Rosado-Ramos, R., Rousseau, C., Sagot, M. F., Dos Santos, C. N., Schmidt, M., Shelenga, T., Shepherd, L., Silva, A. R., da Silva, M. H., Simon, O., Stahlhut, S. G., Solopova, A., Sorokin, A., Stewart, D., Stougie, L., Su, S., Thole, V., Tikhonova, O., Trick, M., Vain, P., Veríssimo, A., Vila-Santa, A., Vinga, S., Vogt, M., Wang, L., Wang, L., Wei, W., Youssef, S., Neves, A. R. & Forster, J., 2018, In : Phytochemistry Reviews. 17, 2, p. 291-326 36 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
1 Downloads (Pure)

TARSyn: Tunable Antibiotic Resistance Devices Enabling Bacterial Synthetic Evolution and Protein Production

Rennig, M., Martinez, V., Mirzadeh, K., Dunas, F., Röjsäter, B., Daley, D. O. & Nørholm, M., 2018, In : A C S Synthetic Biology. 7, 2, p. 432-442 11 p.

Research output: Contribution to journalJournal articleResearchpeer-review

2017

A Modular High-Throughput In Vivo Screening Platform Based on Chimeric Bacterial Receptors

Lehning, C. E., Heidelberger, J. B., Reinhard, J., Nørholm, M. H. H. & Draheim, R. R., 2017, In : A C S Synthetic Biology. 6, 7, p. 1315-1326

Research output: Contribution to journalJournal articleResearchpeer-review

346 Downloads (Pure)

An expression tag toolbox for microbial production of membrane bound plant cytochromes P450

Vazquez Albacete, D., Cavaleiro, M., Christensen, U., Seppala, S., Møller, B. L. & Nørholm, M., 2017, In : Biotechnology and Bioengineering. 114, 4, p. 751-760

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
319 Downloads (Pure)

A versatile one-step CRISPR-Cas9 based approach to plasmid-curing

Lauritsen, I., Porse, A., Sommer, M. O. A. & Nørholm, M., 2017, In : Microbial Cell Factories. 16, 10 p., 135.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
177 Downloads (Pure)

CRISPR/Cas9-based genome editing for simultaneous interference with gene expression and protein stability

Martinez, V., Lauritsen, I., Hobel, T., Li, S., Nielsen, A. T. & Nørholm, M., 2017, In : Nucleic Acids Research. 45, 20, 8 p., gkx797.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File

CRISPR-Cas9 - den revolutionerende gensaks

Lauritsen, I. & Nørholm, M., 2017, In : Dansk Kemi. 98, 8, p. 14-17

Research output: Contribution to journalJournal articleCommunication

1 Downloads (Pure)

De-bugging and maximizing plant cytochrome P450 production in Escherichia coli with C-terminal GFP fusions

Christensen, U., Vazquez Albacete, D., Søgaard, K. M., Hobel, T., Nielsen, M. T., Harrison, S. J., Hansen, A. H., Møller, B. L., Seppala, S. & Nørholm, M., 2017, In : Applied Microbiology and Biotechnology. 101, 10, p. 4103-4113 11 p.

Research output: Contribution to journalJournal articleResearchpeer-review

199 Downloads (Pure)

Increasing the permeability of Escherichia coli using MAC13243

Muheim, C., Götzke, H., Eriksson, A. U., Lindberg, S., Lauritsen, I., Nørholm, M. & Daley, D. O., 2017, In : Scientific Reports. 7, 11 p., 17629.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
240 Downloads (Pure)

Isolation and characterization of the E. coli membrane protein production strain Mutant56(DE3)

Baumgarten, T., Schlegel, S., Wagner, S., Löw, M., Eriksson, J., Bonde, I., Herrgard, M., Heipieper, H. J., Nørholm, M., Slotboom, D. J. & de Gier, J-W., 2017, In : Scientific Reports. 7, 14 p., 45089.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File

The CYP79A1 catalyzed conversion of tyrosine to (E)-p-hydroxyphenylacetaldoxime unravelled using an improved method for homology modeling

Vazquez Albacete, D., Montefiori, M., Kol, S., Motawia, M. S., Møller, B. L., Olsen, L. & Nørholm, M. H. H., 2017, In : Phytochemistry. 135, p. 8-17

Research output: Contribution to journalJournal articleResearchpeer-review

2016
264 Downloads (Pure)

A nanobody:GFP bacterial platform that enables functional enzyme display and easy quantification of display capacity

Wendel, S., Christian Fischer, E., Martinez, V., Seppala, S. & Nørholm, M., 2016, In : Microbial Cell Factories. 15, 13 p., 71.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
209 Downloads (Pure)

Generation of mutation hotspots in ageing bacterial colonies

Sekowska, A., Wendel, S., Christian Fischer, E., Nørholm, M. H. H. & Danchin, A., 2016, In : Scientific Reports. 6, 1, 7 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
1 Downloads (Pure)

SEVA Linkers: A Versatile and Automatable DNA Backbone Exchange Standard for Synthetic Biology

Kim, S. H., Cavaleiro, M., Rennig, M. & Nørholm, M., 2016, In : A C S Synthetic Biology. 5, 10, p. 1177–1181 5 p.

Research output: Contribution to journalJournal articleResearchpeer-review

164 Downloads (Pure)

Side effects of extra tRNA supplied in a typical bacterial protein production scenario

Søgaard, K. M. & Nørholm, M. H. H., 2016, In : Protein Science. 25, 11, p. 2102–2108

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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2015

Accurate DNA assembly and genome engineering with optimized uracil excision cloning

Cavaleiro, M., Kim, S. H., Seppala, S., Nielsen, M. T. & Nørholm, M. H. H., 2015, In : A C S Synthetic Biology. 4, 9, p. 1042-1046 5 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Assembly of Highly Standardized Gene Fragments for High-Level Production of Porphyrins in E. coli

Nielsen, M. T., Madsen, K. M., Seppala, S., Christensen, U., Riisberg, L., Harrison, S. J., Møller, B. L. & Nørholm, M., 2015, In : A C S Synthetic Biology. 4, 3, p. 274-282 9 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Enhanced Protein Production in Escherichia coli by Optimization of Cloning Scars at the Vector-Coding Sequence Junction

Mirzadeh, K., Martinez, V., Toddo, S., Guntur, S., Herrgard, M., Elofsson, A., Nørholm, M. & Daley, D. O., 2015, In : A C S Synthetic Biology. 4, 9, p. 959-965 7 p.

Research output: Contribution to journalJournal articleResearchpeer-review

2014
332 Downloads (Pure)

Microbial Synthesis of the Forskolin Precursor Manoyl Oxide in an Enantiomerically Pure Form

Nielsen, M. T., Ranberg, J. A., Christensen, U., Christensen, H. B., Harrison, S. J., Olsen, C. E., Hamberger, B., Møller, B. L. & Nørholm, M., 2014, In : APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 80, 23, p. 7258–7265 8 p.

Research output: Contribution to journalJournal articleResearchpeer-review

File

‘Forcing the issue’ - Aromatic tuning facilitates stimulus-independent modulation of a two-component signaling circuit

Nørholm, M., von Heijne, G. & Draheim, R. R., 2014, In : A C S Synthetic Biology. 4, 4, p. 474-481 8 p.

Research output: Contribution to journalJournal articleResearchpeer-review

2013

Improved production of membrane proteins in Escherichia coli by selective codon substitutions

Nørholm, M. H. H., Toddo, S., Virkki, M. T. I., Light, S., von Heijne, G. & Daley, D. O., 2013, In : FEBS Letters. 587, 15, p. 2352-2358

Research output: Contribution to journalJournal articleResearchpeer-review

2012
1 Downloads (Pure)

Application of split-green fluorescent protein for topology mapping membrane proteins in Escherichia coli

Toddo, S., Soderstrom, B., Palombo, I., von Heijne, G., Nørholm, M. & Daley, D. O., 2012, In : Protein Science. 21, 10, p. 1571-1576

Research output: Contribution to journalJournal articleResearchpeer-review