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Research Output

2019

Industrializing a Bacterial Strain for l -Serine Production through Translation Initiation Optimization

Rennig, M., Mundhada, H., Wordofa, G. G., Gerngross, D., Wulff, T., Worberg, A., Nielsen, A. T. & Nørholm, M. H. H., 2019, In : ACS Synthetic Biology. 8, 10, p. 2347-2358

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
114 Downloads (Pure)

Meta synthetic biology: controlling the evolution of engineered living systems

Nørholm, M. H. H., 2019, In : Microbial Biotechnology. 12, 1, p. 35-37

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
270 Downloads (Pure)

Mutations in the Global Transcription Factor CRP/CAP: Insights from Experimental Evolution and Deep Sequencing

Frendorf, P. O., Lauritsen, I., Sekowska, A., Danchin, A. & Nørholm, M. H. H., 2019, In : Computational and Structural Biotechnology Journal. 17, p. 730-736

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
141 Downloads (Pure)
2018

A synbio approach for selection of highly expressed gene variants in Gram-positive bacteria

Ferro, R., Rennig, M., Hernández Rollán, C., Daley, D. O. & Nørholm, M. H. H., 2018, In : Microbial Cell Factories. 17, 1, 11 p., 37.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
369 Downloads (Pure)

BacHBerry:: BACterial Hosts for production of Bioactive phenolics from bERRY fruits

Dudnik, A., Almeida, A. F., Andrade, R., Avila, B., Bañados, P., Barbay, D., Bassard, J. E., Benkoulouche, M., Bott, M., Braga, A., Breitel, D., Brennan, R., Bulteau, L., Chanforan, C., Costa, I., Costa, R. S., Doostmohammadi, M., Faria, N., Feng, C., Fernandes, A. & 63 others, Ferreira, P., Ferro, R., Foito, A., Freitag, S., Garcia, G., Gaspar, P., Godinho-Pereira, J., Hamberger, B., Hartmann, A., Heider, H., Jardim, C., Julien-Laferriere, A., Kallscheuer, N., Kerbe, W., Kuipers, O. P., Li, S., Love, N., Marchetti-Spaccamela, A., Marienhagen, J., Martin, C., Mary, A., Mazurek, V., Meinhart, C., Sevillano, D. M., Menezes, R., Naesby, M., Nørholm, M. H. H., Okkels, F. T., Oliveira, J., Ottens, M., Parrot, D., Pei, L., Rocha, I., Rosado-Ramos, R., Rousseau, C., Sagot, M. F., Dos Santos, C. N., Schmidt, M., Shelenga, T., Shepherd, L., Silva, A. R., da Silva, M. H., Simon, O., Stahlhut, S. G., Solopova, A., Sorokin, A., Stewart, D., Stougie, L., Su, S., Thole, V., Tikhonova, O., Trick, M., Vain, P., Veríssimo, A., Vila-Santa, A., Vinga, S., Vogt, M., Wang, L., Wang, L., Wei, W., Youssef, S., Neves, A. R. & Forster, J., 2018, In : Phytochemistry Reviews. 17, 2, p. 291-326 36 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
356 Downloads (Pure)

Bacterial Genome Editing Strategy for Control of Transcription and Protein Stability

Lauritsen, I., Martinez, V., Ronda, C., Nielsen, A. T. & Nørholm, M. H. H., 2018, Synthetic Metabolic Pathways. Vol. 1671. p. 27-37 (Methods in Molecular Biology).

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

LyGo: simple one-step cloning of LPMO expression vectors: from in silico design to lab-ready construct in one week!

Hernández Rollán, C., Falkenberg, K. B., Rennig, M. & Nørholm, M., 2018, Sustain Conference 2018: Creating Technology for a Sustainable Society. C. M. & K. M. (eds.). Lyngby, Denmark: Technical University of Denmark, 1 p. R-8

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsResearchpeer-review

Open Access

Selection of Highly Expressed Gene Variants in Escherichia coli Using Translationally Coupled Antibiotic Selection Markers

Rennig, M., Daley, D. O. & Nørholm, M. H. H., 2018, Synthetic Metabolic Pathways. Vol. 1671. p. 259-268 (Methods in Molecular Biology).

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

Standardized Cloning and Curing of Plasmids

Lauritsen, I., Kim, S. H., Porse, A. & Nørholm, M. H. H., 2018, Synthetic Biology. Methods in Molecular Biology. Braman, J. (ed.). New York: Humana Press, Vol. 1772. p. 469-476 (Methods in Molecular Biology).

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

TARSyn: Tunable Antibiotic Resistance Devices Enabling Bacterial Synthetic Evolution and Protein Production

Rennig, M., Martinez, V., Mirzadeh, K., Dunas, F., Röjsäter, B., Daley, D. O. & Nørholm, M., 2018, In : A C S Synthetic Biology. 7, 2, p. 432-442 11 p.

Research output: Contribution to journalJournal articleResearchpeer-review

1 Downloads (Pure)
2017

A Modular High-Throughput In Vivo Screening Platform Based on Chimeric Bacterial Receptors

Lehning, C. E., Heidelberger, J. B., Reinhard, J., Nørholm, M. H. H. & Draheim, R. R., 2017, In : A C S Synthetic Biology. 6, 7, p. 1315-1326

Research output: Contribution to journalJournal articleResearchpeer-review

An expression tag toolbox for microbial production of membrane bound plant cytochromes P450

Vazquez Albacete, D., Cavaleiro, M., Christensen, U., Seppala, S., Møller, B. L. & Nørholm, M., 2017, In : Biotechnology and Bioengineering. 114, 4, p. 751-760

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
449 Downloads (Pure)

A versatile one-step CRISPR-Cas9 based approach to plasmid-curing

Lauritsen, I., Porse, A., Sommer, M. O. A. & Nørholm, M., 2017, In : Microbial Cell Factories. 16, 10 p., 135.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
393 Downloads (Pure)

CRISPR/Cas9-based genome editing for simultaneous interference with gene expression and protein stability

Martinez, V., Lauritsen, I., Hobel, T., Li, S., Nielsen, A. T. & Nørholm, M., 2017, In : Nucleic Acids Research. 45, 20, 8 p., gkx797.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
245 Downloads (Pure)

CRISPR-Cas9 - den revolutionerende gensaks

Lauritsen, I. & Nørholm, M., 2017, In : Dansk Kemi. 98, 8, p. 14-17

Research output: Contribution to journalJournal articleCommunication

De-bugging and maximizing plant cytochrome P450 production in Escherichia coli with C-terminal GFP fusions

Christensen, U., Vazquez Albacete, D., Søgaard, K. M., Hobel, T., Nielsen, M. T., Harrison, S. J., Hansen, A. H., Møller, B. L., Seppala, S. & Nørholm, M., 2017, In : Applied Microbiology and Biotechnology. 101, 10, p. 4103-4113 11 p.

Research output: Contribution to journalJournal articleResearchpeer-review

1 Downloads (Pure)

Increasing the permeability of Escherichia coli using MAC13243

Muheim, C., Götzke, H., Eriksson, A. U., Lindberg, S., Lauritsen, I., Nørholm, M. & Daley, D. O., 2017, In : Scientific Reports. 7, 11 p., 17629.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
264 Downloads (Pure)

Isolation and characterization of the E. coli membrane protein production strain Mutant56(DE3)

Baumgarten, T., Schlegel, S., Wagner, S., Löw, M., Eriksson, J., Bonde, I., Herrgard, M., Heipieper, H. J., Nørholm, M., Slotboom, D. J. & de Gier, J-W., 2017, In : Scientific Reports. 7, 14 p., 45089.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
310 Downloads (Pure)

The CYP79A1 catalyzed conversion of tyrosine to (E)-p-hydroxyphenylacetaldoxime unravelled using an improved method for homology modeling

Vazquez Albacete, D., Montefiori, M., Kol, S., Motawia, M. S., Møller, B. L., Olsen, L. & Nørholm, M. H. H., 2017, In : Phytochemistry. 135, p. 8-17

Research output: Contribution to journalJournal articleResearchpeer-review

2016

A nanobody:GFP bacterial platform that enables functional enzyme display and easy quantification of display capacity

Wendel, S., Christian Fischer, E., Martinez, V., Seppala, S. & Nørholm, M., 2016, In : Microbial Cell Factories. 15, 13 p., 71.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
349 Downloads (Pure)

Codon optimizing for increased membrane protein production: a minimalist approach

Mirzadeh, K., Toddo, S., Nørholm, M. & Daley, D. O., 2016, Heterologous Expression of Membrane Proteins: Methods and Protocols. Mus-Veteau, I. (ed.). Springer, Vol. 1432. p. 53-61 9 p. (Methods in Molecular Biology).

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

Generation of mutation hotspots in ageing bacterial colonies

Sekowska, A., Wendel, S., Christian Fischer, E., Nørholm, M. H. H. & Danchin, A., 2016, In : Scientific Reports. 6, 1, 7 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
263 Downloads (Pure)

Generation of mutation hotspots in ageing bacterial colonies

Sekowska, A., Wendel, S., Nørholm, M. & Danchin, A., 2016, bioRxiv, 15 p.

Research output: Working paperResearch

File
230 Downloads (Pure)

SEVA Linkers: A Versatile and Automatable DNA Backbone Exchange Standard for Synthetic Biology

Kim, S. H., Cavaleiro, M., Rennig, M. & Nørholm, M., 2016, In : A C S Synthetic Biology. 5, 10, p. 1177–1181 5 p.

Research output: Contribution to journalJournal articleResearchpeer-review

1 Downloads (Pure)

Side effects of extra tRNA supplied in a typical bacterial protein production scenario

Søgaard, K. M. & Nørholm, M. H. H., 2016, In : Protein Science. 25, 11, p. 2102–2108

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
221 Downloads (Pure)
2015

Accurate Dna Assembly And Direct Genome Integration With Optimized Uracil Excision Cloning To Facilitate Engineering Of Escherichia Coli As A Cell Factory

Cavaleiro, M., Kim, S. H. & Nørholm, M., 2015, The Danish Microbiological Society Annual Congress 2015: Programme & Abstracts. Copenhagen, p. 77-77

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsResearchpeer-review

Accurate DNA assembly and genome engineering with optimized uracil excision cloning

Cavaleiro, M., Kim, S. H., Seppala, S., Nielsen, M. T. & Nørholm, M. H. H., 2015, In : A C S Synthetic Biology. 4, 9, p. 1042-1046 5 p.

Research output: Contribution to journalJournal articleResearchpeer-review

An Antibiotic Selection System For Protein Overproducing Bacteria

Rennig, M. & Nørholm, M., 2015, The Danish Microbiological Society Annual Congress 2015: Programme & Abstracts. Copenhagen, p. 76-77

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsResearchpeer-review

1 Downloads (Pure)

Assembly of Highly Standardized Gene Fragments for High-Level Production of Porphyrins in E. coli

Nielsen, M. T., Madsen, K. M., Seppala, S., Christensen, U., Riisberg, L., Harrison, S. J., Møller, B. L. & Nørholm, M., 2015, In : A C S Synthetic Biology. 4, 3, p. 274-282 9 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Conditional protein depletion using small degradation tags and CRISPR/Cas9 systems

Martinez, V., Lauritsen, I., Wolff, T. & Nørholm, M. H. H., 2015, Book of Abstracts. DTU's Sustain Conference 2015. Lyngby: Technical University of Denmark, 1 p. B-4

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsResearchpeer-review

Open Access
File
292 Downloads (Pure)

Enhanced Protein Production in Escherichia coli by Optimization of Cloning Scars at the Vector-Coding Sequence Junction

Mirzadeh, K., Martinez, V., Toddo, S., Guntur, S., Herrgard, M., Elofsson, A., Nørholm, M. & Daley, D. O., 2015, In : A C S Synthetic Biology. 4, 9, p. 959-965 7 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Expanding the N-terminal tag toolbox for functional expression of Cytochrome P450s in cell factories

Vazquez Albacete, D., Cavaleiro, M., Christensen, U., Seppälä, S. & Nørholm, M. H. H., 2015, Book of Abstracts. DTU's Sustain Conference 2015. Lyngby: Technical University of Denmark, 2 p. B-17

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsResearchpeer-review

Open Access
File
86 Downloads (Pure)

Exploring Marine Environments To Unravel Tolerance Mechanisms To Relevant Compounds

Machado, H., Cavaleiro, M., Nørholm, M. & Gram, L., 2015, The Danish Microbiological Society Annual Congress 2015: Programme & Abstracts. Copenhagen, p. 76-76

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsResearchpeer-review

Stereo-specific synthesis of (13r)-manoyl oxide

Hamberger, B., Pateraki, E., Møller, B. L., Nørholm, M., Nielsen, M. T., Ranberg, J. A., Bohlman, C. J. & Zerbe, P., 25 Jun 2015, IPC No. C12N 15/ 52 A I, Patent No. WO2015091943, 25 Jun 2015, Priority date 20 Dec 2013, Priority No. EP20130198742

Research output: Patent

Open Access
File
84 Downloads (Pure)

Superlinkers – A versatile and automatable DNA backbone exchange standard for synthetic biology

Kim, S. H., Cavaleiro, M., Riisberg, L., Smith, P. & Nørholm, M., 2015. 1 p.

Research output: Contribution to conferenceConference abstract for conferenceResearchpeer-review

Open Access
File
271 Downloads (Pure)

Uracil Excision for Assembly of Complex Pathways

Cavaleiro, M., Nielsen, M. T., Kim, S. H., Seppala, S. & Nørholm, M., 2015, Springer Protocols Handbooks. Humana Press, 11 p. (Springer Protocols Handbooks).

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

2014

Microbial Synthesis of the Forskolin Precursor Manoyl Oxide in an Enantiomerically Pure Form

Nielsen, M. T., Ranberg, J. A., Christensen, U., Christensen, H. B., Harrison, S. J., Olsen, C. E., Hamberger, B., Møller, B. L. & Nørholm, M., 2014, In : APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 80, 23, p. 7258–7265 8 p.

Research output: Contribution to journalJournal articleResearchpeer-review

File
401 Downloads (Pure)

Synthetic Biology Tools for the Membrane – Targeted Localisation and Elucidation of Protein Interactions

Wendel, S., Seppala, S. & Nørholm, M., 2014, Abstract Book - DTU Sustain Conference 2014. Kgs. Lyngby: Technical University of Denmark, 1 p.

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsResearchpeer-review

‘Forcing the issue’ - Aromatic tuning facilitates stimulus-independent modulation of a two-component signaling circuit

Nørholm, M., von Heijne, G. & Draheim, R. R., 2014, In : A C S Synthetic Biology. 4, 4, p. 474-481 8 p.

Research output: Contribution to journalJournal articleResearchpeer-review

2013

Improved production of membrane proteins in Escherichia coli by selective codon substitutions

Nørholm, M. H. H., Toddo, S., Virkki, M. T. I., Light, S., von Heijne, G. & Daley, D. O., 2013, In : FEBS Letters. 587, 15, p. 2352-2358

Research output: Contribution to journalJournal articleResearchpeer-review

2012

Application of split-green fluorescent protein for topology mapping membrane proteins in Escherichia coli

Toddo, S., Soderstrom, B., Palombo, I., von Heijne, G., Nørholm, M. & Daley, D. O., 2012, In : Protein Science. 21, 10, p. 1571-1576

Research output: Contribution to journalJournal articleResearchpeer-review

1 Downloads (Pure)