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Research Output 2012 2019

2019
21 Downloads (Pure)

Industrializing a Bacterial Strain for l -Serine Production through Translation Initiation Optimization

Rennig, M., Mundhada, H., Wordofa, G. G., Gerngross, D., Wulff, T., Worberg, A., Nielsen, A. T. & Nørholm, M. H. H., 2019, In : ACS Synthetic Biology. 8, 10, p. 2347-2358

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
198 Downloads (Pure)

Meta synthetic biology: controlling the evolution of engineered living systems

Nørholm, M. H. H., 2019, In : Microbial Biotechnology. 12, 1, p. 35-37

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
80 Downloads (Pure)

Mutations in the Global Transcription Factor CRP/CAP: Insights from Experimental Evolution and Deep Sequencing

Frendorf, P. O., Lauritsen, I., Sekowska, A., Danchin, A. & Nørholm, M. H. H., 2019, In : Computational and Structural Biotechnology Journal. 17, p. 730-736

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
2018
292 Downloads (Pure)

A synbio approach for selection of highly expressed gene variants in Gram-positive bacteria

Ferro, R., Rennig, M., Hernández Rollán, C., Daley, D. O. & Nørholm, M. H. H., 2018, In : Microbial Cell Factories. 17, 1, 11 p., 37.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
241 Downloads (Pure)

BacHBerry:: BACterial Hosts for production of Bioactive phenolics from bERRY fruits

Dudnik, A., Almeida, A. F., Andrade, R., Avila, B., Bañados, P., Barbay, D., Bassard, J. E., Benkoulouche, M., Bott, M., Braga, A., Breitel, D., Brennan, R., Bulteau, L., Chanforan, C., Costa, I., Costa, R. S., Doostmohammadi, M., Faria, N., Feng, C., Fernandes, A. & 63 others, Ferreira, P., Ferro, R., Foito, A., Freitag, S., Garcia, G., Gaspar, P., Godinho-Pereira, J., Hamberger, B., Hartmann, A., Heider, H., Jardim, C., Julien-Laferriere, A., Kallscheuer, N., Kerbe, W., Kuipers, O. P., Li, S., Love, N., Marchetti-Spaccamela, A., Marienhagen, J., Martin, C., Mary, A., Mazurek, V., Meinhart, C., Sevillano, D. M., Menezes, R., Naesby, M., Nørholm, M. H. H., Okkels, F. T., Oliveira, J., Ottens, M., Parrot, D., Pei, L., Rocha, I., Rosado-Ramos, R., Rousseau, C., Sagot, M. F., Dos Santos, C. N., Schmidt, M., Shelenga, T., Shepherd, L., Silva, A. R., da Silva, M. H., Simon, O., Stahlhut, S. G., Solopova, A., Sorokin, A., Stewart, D., Stougie, L., Su, S., Thole, V., Tikhonova, O., Trick, M., Vain, P., Veríssimo, A., Vila-Santa, A., Vinga, S., Vogt, M., Wang, L., Wang, L., Wei, W., Youssef, S., Neves, A. R. & Forster, J., 2018, In : Phytochemistry Reviews. 17, 2, p. 291-326 36 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File

Bacterial Genome Editing Strategy for Control of Transcription and Protein Stability

Lauritsen, I., Martinez, V., Ronda, C., Nielsen, A. T. & Nørholm, M. H. H., 2018, Synthetic Metabolic Pathways. Vol. 1671. p. 27-37 (Methods in Molecular Biology).

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

LyGo: simple one-step cloning of LPMO expression vectors: from in silico design to lab-ready construct in one week!

Hernández Rollán, C., Falkenberg, K. B., Rennig, M. & Nørholm, M., 2018, Sustain Conference 2018: Creating Technology for a Sustainable Society. C. M. & K. M. (eds.). Lyngby, Denmark: Technical University of Denmark, 1 p. R-8

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsResearchpeer-review

Open Access

Selection of Highly Expressed Gene Variants in Escherichia coli Using Translationally Coupled Antibiotic Selection Markers

Rennig, M., Daley, D. O. & Nørholm, M. H. H., 2018, Synthetic Metabolic Pathways. Vol. 1671. p. 259-268 (Methods in Molecular Biology).

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

Standardized Cloning and Curing of Plasmids

Lauritsen, I., Kim, S. H., Porse, A. & Nørholm, M. H. H., 2018, Synthetic Biology. Methods in Molecular Biology. Braman, J. (ed.). New York: Humana Press, Vol. 1772. p. 469-476 (Methods in Molecular Biology).

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

1 Downloads (Pure)

TARSyn: Tunable Antibiotic Resistance Devices Enabling Bacterial Synthetic Evolution and Protein Production

Rennig, M., Martinez, V., Mirzadeh, K., Dunas, F., Röjsäter, B., Daley, D. O. & Nørholm, M., 2018, In : A C S Synthetic Biology. 7, 2, p. 432-442 11 p.

Research output: Contribution to journalJournal articleResearchpeer-review

2017

A Modular High-Throughput In Vivo Screening Platform Based on Chimeric Bacterial Receptors

Lehning, C. E., Heidelberger, J. B., Reinhard, J., Nørholm, M. H. H. & Draheim, R. R., 2017, In : A C S Synthetic Biology. 6, 7, p. 1315-1326

Research output: Contribution to journalJournal articleResearchpeer-review

346 Downloads (Pure)

An expression tag toolbox for microbial production of membrane bound plant cytochromes P450

Vazquez Albacete, D., Cavaleiro, M., Christensen, U., Seppala, S., Møller, B. L. & Nørholm, M., 2017, In : Biotechnology and Bioengineering. 114, 4, p. 751-760

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
319 Downloads (Pure)

A versatile one-step CRISPR-Cas9 based approach to plasmid-curing

Lauritsen, I., Porse, A., Sommer, M. O. A. & Nørholm, M., 2017, In : Microbial Cell Factories. 16, 10 p., 135.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
177 Downloads (Pure)

CRISPR/Cas9-based genome editing for simultaneous interference with gene expression and protein stability

Martinez, V., Lauritsen, I., Hobel, T., Li, S., Nielsen, A. T. & Nørholm, M., 2017, In : Nucleic Acids Research. 45, 20, 8 p., gkx797.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File

CRISPR-Cas9 - den revolutionerende gensaks

Lauritsen, I. & Nørholm, M., 2017, In : Dansk Kemi. 98, 8, p. 14-17

Research output: Contribution to journalJournal articleCommunication

1 Downloads (Pure)

De-bugging and maximizing plant cytochrome P450 production in Escherichia coli with C-terminal GFP fusions

Christensen, U., Vazquez Albacete, D., Søgaard, K. M., Hobel, T., Nielsen, M. T., Harrison, S. J., Hansen, A. H., Møller, B. L., Seppala, S. & Nørholm, M., 2017, In : Applied Microbiology and Biotechnology. 101, 10, p. 4103-4113 11 p.

Research output: Contribution to journalJournal articleResearchpeer-review

199 Downloads (Pure)

Increasing the permeability of Escherichia coli using MAC13243

Muheim, C., Götzke, H., Eriksson, A. U., Lindberg, S., Lauritsen, I., Nørholm, M. & Daley, D. O., 2017, In : Scientific Reports. 7, 11 p., 17629.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
240 Downloads (Pure)

Isolation and characterization of the E. coli membrane protein production strain Mutant56(DE3)

Baumgarten, T., Schlegel, S., Wagner, S., Löw, M., Eriksson, J., Bonde, I., Herrgard, M., Heipieper, H. J., Nørholm, M., Slotboom, D. J. & de Gier, J-W., 2017, In : Scientific Reports. 7, 14 p., 45089.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File

The CYP79A1 catalyzed conversion of tyrosine to (E)-p-hydroxyphenylacetaldoxime unravelled using an improved method for homology modeling

Vazquez Albacete, D., Montefiori, M., Kol, S., Motawia, M. S., Møller, B. L., Olsen, L. & Nørholm, M. H. H., 2017, In : Phytochemistry. 135, p. 8-17

Research output: Contribution to journalJournal articleResearchpeer-review

2016
264 Downloads (Pure)

A nanobody:GFP bacterial platform that enables functional enzyme display and easy quantification of display capacity

Wendel, S., Christian Fischer, E., Martinez, V., Seppala, S. & Nørholm, M., 2016, In : Microbial Cell Factories. 15, 13 p., 71.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File

Codon optimizing for increased membrane protein production: a minimalist approach

Mirzadeh, K., Toddo, S., Nørholm, M. & Daley, D. O., 2016, Heterologous Expression of Membrane Proteins: Methods and Protocols. Mus-Veteau, I. (ed.). Springer, Vol. 1432. p. 53-61 9 p. (Methods in Molecular Biology).

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

176 Downloads (Pure)

Generation of mutation hotspots in ageing bacterial colonies

Sekowska, A., Wendel, S., Nørholm, M. & Danchin, A., 2016, bioRxiv, 15 p.

Research output: Working paperResearch

File
209 Downloads (Pure)

Generation of mutation hotspots in ageing bacterial colonies

Sekowska, A., Wendel, S., Christian Fischer, E., Nørholm, M. H. H. & Danchin, A., 2016, In : Scientific Reports. 6, 1, 7 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
1 Downloads (Pure)

SEVA Linkers: A Versatile and Automatable DNA Backbone Exchange Standard for Synthetic Biology

Kim, S. H., Cavaleiro, M., Rennig, M. & Nørholm, M., 2016, In : A C S Synthetic Biology. 5, 10, p. 1177–1181 5 p.

Research output: Contribution to journalJournal articleResearchpeer-review

164 Downloads (Pure)

Side effects of extra tRNA supplied in a typical bacterial protein production scenario

Søgaard, K. M. & Nørholm, M. H. H., 2016, In : Protein Science. 25, 11, p. 2102–2108

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
2015

Accurate Dna Assembly And Direct Genome Integration With Optimized Uracil Excision Cloning To Facilitate Engineering Of Escherichia Coli As A Cell Factory

Cavaleiro, M., Kim, S. H. & Nørholm, M., 2015, The Danish Microbiological Society Annual Congress 2015: Programme & Abstracts. Copenhagen, p. 77-77

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsResearchpeer-review

Accurate DNA assembly and genome engineering with optimized uracil excision cloning

Cavaleiro, M., Kim, S. H., Seppala, S., Nielsen, M. T. & Nørholm, M. H. H., 2015, In : A C S Synthetic Biology. 4, 9, p. 1042-1046 5 p.

Research output: Contribution to journalJournal articleResearchpeer-review

1 Downloads (Pure)

An Antibiotic Selection System For Protein Overproducing Bacteria

Rennig, M. & Nørholm, M., 2015, The Danish Microbiological Society Annual Congress 2015: Programme & Abstracts. Copenhagen, p. 76-77

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsResearchpeer-review

Assembly of Highly Standardized Gene Fragments for High-Level Production of Porphyrins in E. coli

Nielsen, M. T., Madsen, K. M., Seppala, S., Christensen, U., Riisberg, L., Harrison, S. J., Møller, B. L. & Nørholm, M., 2015, In : A C S Synthetic Biology. 4, 3, p. 274-282 9 p.

Research output: Contribution to journalJournal articleResearchpeer-review

284 Downloads (Pure)

Conditional protein depletion using small degradation tags and CRISPR/Cas9 systems

Martinez, V., Lauritsen, I., Wolff, T. & Nørholm, M. H. H., 2015, Book of Abstracts. DTU's Sustain Conference 2015. Lyngby: Technical University of Denmark, 1 p. B-4

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsResearchpeer-review

Open Access
File

Enhanced Protein Production in Escherichia coli by Optimization of Cloning Scars at the Vector-Coding Sequence Junction

Mirzadeh, K., Martinez, V., Toddo, S., Guntur, S., Herrgard, M., Elofsson, A., Nørholm, M. & Daley, D. O., 2015, In : A C S Synthetic Biology. 4, 9, p. 959-965 7 p.

Research output: Contribution to journalJournal articleResearchpeer-review

68 Downloads (Pure)

Expanding the N-terminal tag toolbox for functional expression of Cytochrome P450s in cell factories

Vazquez Albacete, D., Cavaleiro, M., Christensen, U., Seppälä, S. & Nørholm, M. H. H., 2015, Book of Abstracts. DTU's Sustain Conference 2015. Lyngby: Technical University of Denmark, 2 p. B-17

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsResearchpeer-review

Open Access
File

Exploring Marine Environments To Unravel Tolerance Mechanisms To Relevant Compounds

Machado, H., Cavaleiro, M., Nørholm, M. & Gram, L., 2015, The Danish Microbiological Society Annual Congress 2015: Programme & Abstracts. Copenhagen, p. 76-76

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsResearchpeer-review

75 Downloads (Pure)

Stereo-specific synthesis of (13r)-manoyl oxide

Hamberger, B., Pateraki, E., Møller, B. L., Nørholm, M., Nielsen, M. T., Ranberg, J. A., Bohlman, C. J. & Zerbe, P., 25 Jun 2015, IPC No. C12N 15/ 52 A I, Patent No. WO2015091943, 25 Jun 2015, Priority date 20 Dec 2013, Priority No. EP20130198742

Research output: Patent

Open Access
File
256 Downloads (Pure)

Superlinkers – A versatile and automatable DNA backbone exchange standard for synthetic biology

Kim, S. H., Cavaleiro, M., Riisberg, L., Smith, P. & Nørholm, M., 2015. 1 p.

Research output: Contribution to conferenceConference abstract for conferenceResearchpeer-review

Open Access
File

Uracil Excision for Assembly of Complex Pathways

Cavaleiro, M., Nielsen, M. T., Kim, S. H., Seppala, S. & Nørholm, M., 2015, Springer Protocols Handbooks. Humana Press, 11 p. (Springer Protocols Handbooks).

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

2014
332 Downloads (Pure)

Microbial Synthesis of the Forskolin Precursor Manoyl Oxide in an Enantiomerically Pure Form

Nielsen, M. T., Ranberg, J. A., Christensen, U., Christensen, H. B., Harrison, S. J., Olsen, C. E., Hamberger, B., Møller, B. L. & Nørholm, M., 2014, In : APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 80, 23, p. 7258–7265 8 p.

Research output: Contribution to journalJournal articleResearchpeer-review

File

Synthetic Biology Tools for the Membrane – Targeted Localisation and Elucidation of Protein Interactions

Wendel, S., Seppala, S. & Nørholm, M., 2014, Abstract Book - DTU Sustain Conference 2014. Kgs. Lyngby: Technical University of Denmark, 1 p.

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsResearchpeer-review

‘Forcing the issue’ - Aromatic tuning facilitates stimulus-independent modulation of a two-component signaling circuit

Nørholm, M., von Heijne, G. & Draheim, R. R., 2014, In : A C S Synthetic Biology. 4, 4, p. 474-481 8 p.

Research output: Contribution to journalJournal articleResearchpeer-review

2013

Improved production of membrane proteins in Escherichia coli by selective codon substitutions

Nørholm, M. H. H., Toddo, S., Virkki, M. T. I., Light, S., von Heijne, G. & Daley, D. O., 2013, In : FEBS Letters. 587, 15, p. 2352-2358

Research output: Contribution to journalJournal articleResearchpeer-review

2012
1 Downloads (Pure)

Application of split-green fluorescent protein for topology mapping membrane proteins in Escherichia coli

Toddo, S., Soderstrom, B., Palombo, I., von Heijne, G., Nørholm, M. & Daley, D. O., 2012, In : Protein Science. 21, 10, p. 1571-1576

Research output: Contribution to journalJournal articleResearchpeer-review