• Kogle Allé 6, Cederhuset, xx

    2970 Hørsholm

    Denmark

20152020

Research output per year

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Research Output

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Journal article

13C Metabolic Flux Analysis for Systematic Metabolic Engineering of S. cerevisiae for Overproduction of Fatty Acids

Ghosh, A., Ando, D., Gin, J., Runguphan, W., Denby, C., Wang, G., Baidoo, E. E. K., Shymansky, C., Keasling, J. & García Martín, H., 2016, In : Frontiers in Bioengineering and Biotechnology. 4, 10 p., 76.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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259 Downloads (Pure)

A Cas9-based toolkit to program gene expression in Saccharomyces cerevisiae

Apel, A. R., d'Espaux, L., Wehrs, M., Sachs, D., Li, R. A., Tong, G. J., Garber, M., Nnadi, O., Zhuang, W., Hillson, N. J., Keasling, J. D. & Mukhopadhyay, A., 2017, In : Nucleic acids research. 45, 1, p. 496-508

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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199 Downloads (Pure)

A combinatorial approach to synthetic transcription factor-promoter combinations for yeast strain engineering

Dossani, Z. Y., Apel, A. R., Szmidt-Middleton, H., Hillson, N. J., Deutsch, S., Keasling, J. D. & Mukhopadhyay, A., 2018, In : Yeast. 35, 3, p. 273-280

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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386 Downloads (Pure)

A High-Throughput Mass Spectrometric Enzyme Activity Assay Enabling the Discovery of Cytochrome P450 Biocatalysts

de Rond, T., Gao, J., Zargar, A., de Raad, M., Cunha, J., Northen, T. R. & Keasling, J. D., 2019, In : ANGEWANDTE CHEMIE. 58, 30, p. 10114-10119 7 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Application of an Acyl-CoA Ligase from Streptomyces aizunensis for Lactam Biosynthesis

Zhang, J., Barajas, J. F., Burdu, M., Wang, G., Baidoo, E. E. & Keasling, J. D., 2017, In : A C S Synthetic Biology. 6, 5, p. 884-890

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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162 Downloads (Pure)

A Pseudomonas putida efflux pump acts on short-chain alcohols

Basler, G., Thompson, M., Tullman-Ercek, D. & Keasling, J., 2018, In : Biotechnology for Biofuels. 11, 1, 10 p., 136.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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168 Downloads (Pure)

A rapid methods development workflow for high-throughput quantitative proteomic applications

Chen, Y., Vu, J., Thompson, M. G., Sharpless, W. A., Chan, L. J. G., Gin, J. W., Keasling, J. D., Adams, P. D. & Petzold, C. J., 2019, In : PLOS ONE. 14, 2, 16 p., e0211582.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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169 Downloads (Pure)

Automated "cells-To-Peptides" Sample Preparation Workflow for High-Throughput, Quantitative Proteomic Assays of Microbes

Chen, Y., Guenther, J. M., Gin, J. W., Chan, L. J. G., Costello, Z., Ogorzalek, T. L., Tran, H. M., Blake-Hedges, J. M., Keasling, J. D., Adams, P. D., García Martín, H., Hillson, N. J. & Petzold, C. J., 2019, In : Journal of Proteome Research. 18, 10, p. 3752-3761 10 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Autonomous control of metabolic state by a quorum sensing (QS)-mediated regulator for bisabolene production in engineered E. coli

Kim, E-M., Min Woo, H., Tian, T., Yilmaz, S., Javidpour, P., Keasling, J. D. & Soon Lee, T., 2017, In : Metabolic Engineering. 44, p. 325-336

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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209 Downloads (Pure)

Base-Catalyzed Depolymerization of Solid Lignin-Rich Streams Enables Microbial Conversion

Rodriguez, A., Salvachúa, D., Katahira, R., Black, B. A., Cleveland, N. S., Reed, M., Smith, H., Baidoo, E. E. K., Keasling, J. D., Simmons, B. A., Beckham, G. T. & Gladden, J. M., 2017, In : A C S Sustainable Chemistry & Engineering. 5, 9, p. 8171-8180

Research output: Contribution to journalJournal articleResearchpeer-review

Biochemical Characterization of β-Amino Acid Incorporation in Fluvirucin B2 Biosynthesis

Barajas, J. F., Zargar, A., Pang, B., Benites, V. T., Gin, J., Baidoo, E. E. K., Petzold, C. J., Hillson, N. J. & Keasling, J. D., 2018, In : Chembiochem. 19, 13, p. 1391-1395

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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41 Downloads (Pure)

Building a global alliance of biofoundries

Hillson, N., Caddick, M., Cai, Y., Carrasco, J. A., Chang, M. W., Curach, N. C., Bell, D. J., Le Feuvre, R., Friedman, D. C., Fu, X., Gold, N. D., Herrgård, M. J., Holowko, M. B., Johnson, J. R., Johnson, R. A., Keasling, J. D., Kitney, R. I., Kondo, A., Liu, C., Martin, V. J. J. & 16 others, Menolascina, F., Ogino, C., Patron, N. J., Pavan, M., Poh, C. L., Pretorius, I. S., Rosser, S. J., Scrutton, N. S., Storch, M., Tekotte, H., Travnik, E., Vickers, C. E., Yew, W. S., Yuan, Y., Zhao, H. & Freemont, P. S., 2019, In : Nature Communications. 10, 2040.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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103 Downloads (Pure)

CasEMBLR: Cas9-Facilitated Multiloci Genomic Integration of in Vivo Assembled DNA Parts in Saccharomyces cerevisiae

Jakociunas, T., Rajkumar, A. S., Zhang, J., Arsovska, D., Rodriguez Prado, E. A., Jendresen, C. B., Skjødt, M. L., Nielsen, A. T., Borodina, I., Jensen, M. K. & Keasling, J., 2015, In : A C S Synthetic Biology. 4, 11, p. 1226-1234 9 p.

Research output: Contribution to journalJournal articleResearchpeer-review

CasPER, a method for directed evolution in genomic contexts using mutagenesis and CRISPR/Cas9

Jakočiūnas, T., Pedersen, L. E., Lis, A. V., Jensen, M. K. & Keasling, J. D., 2018, In : Metabolic Engineering. 48, p. 288-296

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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371 Downloads (Pure)

ClusterCAD: a computational platform for type I modular polyketide synthase design

Eng, C. H., Backman, T. W. H., Bailey, C. B., Magnan, C., Martin, H. G., Katz, L., Baldi, P. & Keasling, J. D., 2018, In : Nucleic acids research. 46, D1, p. D509-D515

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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126 Downloads (Pure)

Comprehensive in Vitro Analysis of Acyltransferase Domain Exchanges in Modular Polyketide Synthases and Its Application for Short-Chain Ketone Production

Yuzawa, S., Deng, K., Wang, G., Baidoo, E. E. K., Northen, T. R., Adams, P. D., Katz, L. & Keasling, J., 2017, In : A C S Synthetic Biology. 6, 1, p. 139-147

Research output: Contribution to journalJournal articleResearchpeer-review

1 Downloads (Pure)

Constraining genome-scale models to represent the bow tie structure of metabolism for 13C metabolic flux analysis

Backman, T. W. H., Ando, D., Singh, J., Keasling, J. D. & Martín, H. G., 2018, In : Metabolites. 8, 1, 17 p., 3.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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151 Downloads (Pure)
Open Access
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197 Downloads (Pure)

CrEdit: CRISPR mediated multi-loci gene integration in Saccharomyces cerevisiae

Ronda, C., Maury, J., Jakociunas, T., Jacobsen, S. A. B., Germann, S. M., Harrison, S. J., Borodina, I., Keasling, J. D., Jensen, M. K. & Nielsen, A. T., 2015, In : Microbial Cell Factories. 14, 97, 11 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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427 Downloads (Pure)

CRISPR/Cas9 advances engineering of microbial cell factories

Jakociunas, T., Jensen, M. K. & Keasling, J. D., 2016, In : Metabolic Engineering. 34, p. 44-59 16 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Cyanobacterial carbon metabolism: Fluxome plasticity and oxygen dependence

Wan, N., Delorenzo, D. M., He, L., You, L., Immethun, C. M., Wang, G., Baidoo, E. E. K., Hollinshead, W. D., Keasling, J. D., Moon, T. S. & Tang, Y. J., 2017, In : Biotechnology and Bioengineering. 114, 7, p. 1593-1602

Research output: Contribution to journalJournal articleResearchpeer-review

Deciphering flux adjustments of engineered E. coli cells during fermentation with changing growth conditions

He, L., Xiu, Y., Jones, J. A., Baidoo, E. E. K., Keasling, J. D., Tang, Y. J. & Koffas, M. A. G., 2017, In : Metabolic Engineering. 39, p. 247–256 10 p.

Research output: Contribution to journalJournal articleResearchpeer-review

De novo DNA synthesis using polymerasenucleotide conjugates

Palluk, S., Arlow, D. H., De Rond, T., Barthel, S., Kang, J. S., Bector, R., Baghdassarian, H. M., Truong, A. N., Kim, P. W., Singh, A. K., Hillson, N. J. & Keasling, J. D., 2018, In : Nature Biotechnology. 36, 7, p. 645-650

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access

Development of an integrated approach for α-pinene recovery and sugar production from loblolly pine using ionic liquids

Papa, G., Kirby, J., Murthy Konda, N. V. S. N., Tran, K., Singh, S., Keasling, J. D., Peter, G. F. & Simmons, B. A., 2017, In : Green Chemistry. 19, p. 1117-1127

Research output: Contribution to journalJournal articleResearchpeer-review

1 Downloads (Pure)

Development of a Transcription Factor-Based Lactam Biosensor

Zhang, J., Barajas, J. F., Burdu, M., Ruegg, T. L., Dias, B. & Keasling, J., 2017, In : A C S Synthetic Biology. 6, 3, p. 439-445

Research output: Contribution to journalJournal articleResearchpeer-review

Development of biosensors and their application in metabolic engineering

Zhang, J., Jensen, M. K. & Keasling, J., 2015, In : Current Opinion in Chemical Biology. 28, 8 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Development of Next Generation Synthetic Biology Tools for Use in Streptomyces venezuelae

Phelan, R. M., Sachs, D., Petkiewicz, S. J., Barajas, J. F., Blake-Hedges, J. M., Thompson, M. G., Apel, A. R., Rasor, B. J., Katz, L. & Keasling, J. D., 2017, In : A C S Synthetic Biology. 6, 1, p. 159-166

Research output: Contribution to journalJournal articleResearchpeer-review

Discovery of enzymes for toluene synthesis from anoxic microbial communities

Beller, H. R., Rodrigues, A. V., Zargar, K., Wu, Y-W., Saini, A. K., Saville, R. M., Pereira, J. H., Adams, P. D., Tringe, S. G., Petzold, C. J. & Keasling, J. D., 2018, In : Nature Chemical Biology. 14, p. 451-457

Research output: Contribution to journalJournal articleResearchpeer-review

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63 Downloads (Pure)

Distinct functional roles for hopanoid composition in the chemical tolerance of Zymomonas mobilis

Brenac, L., Baidoo, E. E. K., Keasling, J. D. & Budin, I., 2019, In : Molecular Microbiology. 112, 5, p. 1564-1575 12 p.

Research output: Contribution to journalJournal articleResearchpeer-review

EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9

Fabre, M. M. J., Jakociunas, T., Stovicek, V., Dai, Z., Jensen, M. K., Keasling, J. & Borodina, I., 2016, In : Biotechnology Journal. 11, 8, p. 1110-1117 11 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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737 Downloads (Pure)

Engineered polyketides: Synergy between protein and host level engineering

Barajas, J. F., Blake-Hedges, J. M., Bailey, C. B., Curran, S. & Keasling, J. D., 2017, In : Synthetic and Systems Biotechnology. 2, 3, p. 147-166

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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185 Downloads (Pure)

Engineered Production of Short-Chain Acyl-Coenzyme A Esters in Saccharomyces cerevisiae

Krink-Koutsoubelis, N., Loechner, A. C., Lechner, A., Link, H., Denby, C. M., Vögeli, B., Erb, T. J., Yuzawa, S., Jakociunas, T., Katz, L., Jensen, M. K., Sourjik, V. & Keasling, J. D., 2018, In : A C S Synthetic Biology. 7, 4, p. 1105-1115 11 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Engineered Reversal of Function in Glycolytic Yeast Promoters

Rajkumar, A. S., Özdemir, E., Lis, A. V., Schneider, K., Qin, J., Jensen, M. K. & Keasling, J. D., 2019, In : ACS Synthetic Biology. 8, 6, p. 1462-1468 7 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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107 Downloads (Pure)

Engineering a functional 1-deoxy-D-xylulose 5-phosphate (DXP) pathway in Saccharomyces cerevisiae

Kirby, J., Dietzel, K. L., Wichmann, G., Chan, R., Antipov, E., Moss, N., Baidoo, E. E. K., Jackson, P., Gaucher, S. P., Gottlieb, S., LaBarge, J., Mahatdejkul, T., Hawkins, K. M., Muley, S., Newman, J. D., Liu, P., Keasling, J. & Zhao, L., 2016, In : Metabolic Engineering. 38, p. 494-503 10 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Engineering an NADPH/NADPRedox Biosensor in Yeast

Zhang, J., Sonnenschein, N., Pihl, T. P. B., Pedersen, K. R., Jensen, M. K. & Keasling, J., 2016, In : A C S Synthetic Biology. 5, 12, p. 1546–1556 11 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Engineering Bacteria to Catabolize the Carbonaceous Component of Sarin: Teaching E. coli to Eat Isopropanol

Brown, M. E., Mukhopadhyay, A. & Keasling, J. D., 2016, In : A C S Synthetic Biology. 5, 12, p. 1485-1496 12 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Engineering glucose metabolism of escherichia coli under nitrogen starvation

Chubukov, V., Desmarais, J. J., Wang, G., Chan, L. J. G., Baidoo, E. E. K., Petzold, C. J., Keasling, J. D. & Mukhopadhyay, A., 2017, In : n p j Systems Biology and Applications. 3, 1, 7 p., 16035.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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23 Downloads (Pure)

Engineering high-level production of fatty alcohols by Saccharomyces cerevisiae from lignocellulosic feedstocks

d'Espaux, L., Ghosh, A., Runguphan, W., Wehrs, M., Xu, F., Konzock, O., Dev, I., Nhan, M., Gin, J., Apel, A. R., Petzold, C. J., Singh, S., Simmons, B. A., Mukhopadhyay, A., Martín, H. G. & Keasling, J. D., 2017, In : Metabolic Engineering. 42, p. 115-125

Research output: Contribution to journalJournal articleResearchpeer-review

Engineering of synthetic, stress-responsive yeast promoters

Rajkumar, A. S., Liu, G., Bergenholm, D., Arsovska, D., Kristensen, M., Nielsen, J., Jensen, M. K. & Keasling, J., 2016, In : Nucleic acids research. 44, 17, 12 p., e136.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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297 Downloads (Pure)

Engineering prokaryotic transcriptional activators as metabolite biosensors in yeast

Skjødt, M. L., Snoek, T., Kildegaard, K. R., Arsovska, D., Eichenberger, M., Goedecke, T. J., Rajkumar, A. S., Zhang, J., Kristensen, M., Lehka, B., Siedler, S., Borodina, I., Jensen, M. K. & Keasling, J., 2016, In : Nature Chemical Biology. 12, 11, p. 951-958 10 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Engineering β-oxidation in Yarrowia lipolytica for methyl ketone production

Hanko, E. K. R., Denby, C. M., Nogué, V. S. I., Lin, W., Ramirez, K. J., Singer, C. A., Beckham, G. T. & Keasling, J. D., 2018, In : Metabolic Engineering. 48, p. 52-62

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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102 Downloads (Pure)

Enhanced fatty acid production in engineered chemolithoautotrophic bacteria using reduced sulfur compounds as energy sources

Beller, H. R., Zhou, P., Jewell, T. N. M., Goh, E-B. & Keasling, J., 2016, In : Metabolic Engineering Communications. 3, p. 211-215 5 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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399 Downloads (Pure)

Evolution-guided engineering of small-molecule biosensors

Snoek, T., Chaberski, E. K., Ambri, F., Kol, S., Bjørn, S. P., Pang, B., Barajas, J. F., Welner, D. H., Jensen, M. K. & Keasling, J. D., 2020, In : Nucleic acids research. 48, 1, e3.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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20 Downloads (Pure)

Examining Escherichia coli glycolytic pathways, catabolite repression, and metabolite channeling using Δpfk mutants

Hollinshead, W. D., Rodriguez, S., Martin, H. G., Wang, G., Baidoo, E. E. K., Sale, K. L., Keasling, J., Mukhopadhyay, A. & Tang, Y. J., 2016, In : Biotechnology for Biofuels. 9, 13 p., 212.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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201 Downloads (Pure)

Exploiting members of the BAHD acyltransferase family to synthesize multiple hydroxycinnamate and benzoate conjugates in yeast

Eudes, A., Mouille, M., Robinson, D. S., Benites, V. T., Wang, G., Roux, L., Tsai, Y-L., Baidoo, E. E. K., Chiu, T-Y., Heazlewood, J. L., Scheller, H. V., Mukhopadhyay, A., Keasling, J. D., Deutsch, S. & Loque, D., 2016, In : Microbial Cell Factories. 15, 16 p., 198.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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116 Downloads (Pure)

Exploring small-scale chemostats to scale up microbial processes: 3-hydroxypropionic acid production in S. cerevisiae

Lis, A. V., Schneider, K., Weber, J., Keasling, J. D., Jensen, M. K. & Klein, T., 2019, In : Microbial Cell Factories. 18, 1, 11 p., 50.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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165 Downloads (Pure)

Flux-Enabled Exploration of the Role of Sip1 in galactose yeast metabolism

Shymansky, C. M., Wang, G., Baidoo, E. E. K., Gin, J., Apel, A. R., Mukhopadhyay, A., Martin, H. G. & Keasling, J., 2017, In : Frontiers in Bioengineering and Biotechnology. 5, 13 p., 31.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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148 Downloads (Pure)

Heterologous Gene Expression of N-Terminally Truncated Variants of LipPks1 Suggests a Functionally Critical Structural Motif in the N-terminus of Modular Polyketide Synthase

Yuzawa, S., Bailey, C. B., Fujii, T. A., Jocic, R., Barajas, J. F., Benites, V. T., Baidoo, E. E. K., Chen, Y., Petzold, C. J., Katz, L. & Keasling, J. D., 2017, In : A C S Chemical Biology. 12, 11, p. 2725-2729 5 p.

Research output: Contribution to journalJournal articleResearchpeer-review

High-throughput enzyme screening platform for the IPP-bypass mevalonate pathway for isopentenol production

Kang, A., Meadows, C. W., Canu, N., Keasling, J. D. & Lee, T. S., 2017, In : Metabolic Engineering. 41, p. 125-134

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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171 Downloads (Pure)

Improving methyl ketone production in Escherichia coli by heterologous expression of NADH-dependent FabG

Goh, E. B., Chen, Y., Petzold, C. J., Keasling, J. D. & Beller, H. R., 2018, In : Biotechnology and Bioengineering. 115, 5, p. 1161-1172

Research output: Contribution to journalJournal articleResearchpeer-review