• Kogle Allé 6, Cederhuset, xx

    2970 Hørsholm

    Denmark

20152020

Research output per year

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Journal article
2020

Evolution-guided engineering of small-molecule biosensors

Snoek, T., Chaberski, E. K., Ambri, F., Kol, S., Bjørn, S. P., Pang, B., Barajas, J. F., Welner, D. H., Jensen, M. K. & Keasling, J. D., 2020, In : Nucleic acids research. 48, 1, e3.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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96 Downloads (Pure)

High-resolution scanning of optimal biosensor reporter promoters in yeast

Ambri, F., D'ambrosio, V., Di Blasi, R., Maury, J., Baallal Jacobsen, S. A., McCloskey, D., Jensen, M. K. & Keasling, J. D., 2020, In : ACS Synthetic Biology. 9, 2, p. 218-226

Research output: Contribution to journalJournal articleResearchpeer-review

Insights into the Mechanism of Phenylacetate Decarboxylase (PhdB), a Toluene-Producing Glycyl Radical Enzyme

Rodrigues, A. V., Tantillo, D. J., Mukhopadhyay, A., Mukhopadhyay, A., Keasling, J. & Beller, H. R., 2020, In : ChemBioChem. 21, 5, p. 663-671

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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39 Downloads (Pure)

Leveraging host metabolism for bisdemethoxycurcumin production in Pseudomonas putida

Incha, M. R., Thompson, M. G., Blake-Hedges, J. M., Liu, Y., Pearson, A. N., Schmidt, M., Gin, J. W., Petzold, C. J., Deutschbauer, A. M. & Keasling, J. D., 2020, In : Metabolic Engineering Communications. 10, e00119.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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58 Downloads (Pure)

Structural Mechanism of Regioselectivity in an Unusual Bacterial Acyl-CoA Dehydrogenase

Blake-Hedges, J. M., Pereira, J. H., Cruz-Morales, P., Thompson, M. G., Barajas, J. F., Chen, J., Krishna, R. N., Chan, L. J. G., Nimlos, D., Alonso-Martinez, C., Baidoo, E. E. K., Chen, Y., Gin, J. W., Katz, L., Petzold, C. J., Adams, P. D. & Keasling, J. D., 2020, In : Journal of the American Chemical Society. 142, 2, p. 835-846

Research output: Contribution to journalJournal articleResearchpeer-review

2019

A High-Throughput Mass Spectrometric Enzyme Activity Assay Enabling the Discovery of Cytochrome P450 Biocatalysts

de Rond, T., Gao, J., Zargar, A., de Raad, M., Cunha, J., Northen, T. R. & Keasling, J. D., 2019, In : ANGEWANDTE CHEMIE. 58, 30, p. 10114-10119 7 p.

Research output: Contribution to journalJournal articleResearchpeer-review

A rapid methods development workflow for high-throughput quantitative proteomic applications

Chen, Y., Vu, J., Thompson, M. G., Sharpless, W. A., Chan, L. J. G., Gin, J. W., Keasling, J. D., Adams, P. D. & Petzold, C. J., 2019, In : PLOS ONE. 14, 2, 16 p., e0211582.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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226 Downloads (Pure)

Automated "cells-To-Peptides" Sample Preparation Workflow for High-Throughput, Quantitative Proteomic Assays of Microbes

Chen, Y., Guenther, J. M., Gin, J. W., Chan, L. J. G., Costello, Z., Ogorzalek, T. L., Tran, H. M., Blake-Hedges, J. M., Keasling, J. D., Adams, P. D., García Martín, H., Hillson, N. J. & Petzold, C. J., 2019, In : Journal of Proteome Research. 18, 10, p. 3752-3761 10 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Building a global alliance of biofoundries

Hillson, N., Caddick, M., Cai, Y., Carrasco, J. A., Chang, M. W., Curach, N. C., Bell, D. J., Le Feuvre, R., Friedman, D. C., Fu, X., Gold, N. D., Herrgård, M. J., Holowko, M. B., Johnson, J. R., Johnson, R. A., Keasling, J. D., Kitney, R. I., Kondo, A., Liu, C., Martin, V. J. J. & 16 others, Menolascina, F., Ogino, C., Patron, N. J., Pavan, M., Poh, C. L., Pretorius, I. S., Rosser, S. J., Scrutton, N. S., Storch, M., Tekotte, H., Travnik, E., Vickers, C. E., Yew, W. S., Yuan, Y., Zhao, H. & Freemont, P. S., 2019, In : Nature Communications. 10, 2040.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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216 Downloads (Pure)

Coupling S-adenosylmethionine-dependent methylation to growth: Design and uses

Luo, H., Hansen, A. S. L., Yang, L., Schneider, K., Kristensen, M., Christensen, U., Christensen, H. B., Du, B., Özdemir, E., Feist, A. M., Keasling, J. D., Jensen, M. K., Herrgård, M. J. & Palsson, B. O., 2019, In : PLOS Biology. 17, 3, e2007050.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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278 Downloads (Pure)

Distinct functional roles for hopanoid composition in the chemical tolerance of Zymomonas mobilis

Brenac, L., Baidoo, E. E. K., Keasling, J. D. & Budin, I., 2019, In : Molecular Microbiology. 112, 5, p. 1564-1575 12 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Engineered Reversal of Function in Glycolytic Yeast Promoters

Rajkumar, A. S., Özdemir, E., Lis, A. V., Schneider, K., Qin, J., Jensen, M. K. & Keasling, J. D., 2019, In : ACS Synthetic Biology. 8, 6, p. 1462-1468 7 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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197 Downloads (Pure)

Exploring small-scale chemostats to scale up microbial processes: 3-hydroxypropionic acid production in S. cerevisiae

Lis, A. V., Schneider, K., Weber, J., Keasling, J. D., Jensen, M. K. & Klein, T., 2019, In : Microbial Cell Factories. 18, 1, 11 p., 50.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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230 Downloads (Pure)

Massively parallel fitness profiling reveals multiple novel enzymes in Pseudomonas putida lysine metabolism

Thompson, M. G., Blake-Hedges, J. M., Cruz-Morales, P., Barajas, J. F., Curran, S. C., Eiben, C. B., Harris, N. C., Benites, V. T., Gin, J. W., Sharpless, W. A., Twigg, F. F., Skyrud, W., Krishna, R. N., Pereira, J. H., Baidoo, E. E. K., Petzold, C. J., Adams, P. D., Arkin, A. P., Deutschbauer, A. M. & Keasling, J. D., 2019, In : mBio. 10, 3, 17 p., e02577-18.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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96 Downloads (Pure)

Mevalonate Pathway Promiscuity Enables Noncanonical Terpene Production

Eiben, C. B., De Rond, T., Bloszies, C., Gin, J., Chiniquy, J., Baidoo, E. E. K., Petzold, C. J., Hillson, N. J., Fiehn, O. & Keasling, J. D., 2019, In : ACS Synthetic Biology. 8, 10, p. 2238-2247 10 p.

Research output: Contribution to journalJournal articleResearchpeer-review

File
8 Downloads (Pure)

Omics-driven identification and elimination of valerolactam catabolism in Pseudomonas putida KT2440 for increased product titer

Thompson, M. G., Valencia, L. E., Blake-Hedges, J. M., Cruz-Morales, P., Velasquez, A. E., Pearson, A. N., Sermeno, L. N., Sharpless, W. A., Benites, V. T., Chen, Y., Baidoo, E. E. K., Petzold, C. J., Deutschbauer, A. M. & Keasling, J. D., 2019, In : Metabolic Engineering Communications. 9, e00098.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access

Optimization of the IPP-bypass mevalonate pathway and fed-batch fermentation for the production of isoprenol in Escherichia coli

Kang, A., Mendez-Perez, D., Goh, E. B., Baidoo, E. E. K., Benites, V. T., Beller, H. R., Keasling, J. D., Adams, P. D., Mukhopadhyay, A. & Lee, T. S., 2019, In : Metabolic Engineering. 56, p. 85-96 12 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Robust characterization of two distinct glutarate sensing transcription factors of pseudomonas putida L -Lysine metabolism

Thompson, M. G., Costello, Z., Hummel, N. F. C., Cruz-Morales, P., Blake-Hedges, J. M., Krishna, R. N., Skyrud, W., Pearson, A. N., Incha, M. R., Shih, P. M., Garcia-Martin, H. & Keasling, J. D., 2019, In : ACS Synthetic Biology. 8, 10, p. 2385-2396

Research output: Contribution to journalJournal articleResearchpeer-review

Structural insights into dehydratase substrate selection for the borrelidin and fluvirucin polyketide synthases

Barajas, J. F., McAndrew, R. P., Thompson, M. G., Backman, T. W. H., Pang, B., de Rond, T., Pereira, J. H., Benites, V. T., Martín, H. G., Baidoo, E. E. K., Hillson, N. J., Adams, P. D. & Keasling, J. D., 2019, In : Journal of Industrial Microbiology and Biotechnology. 46, 8, p. 1225-1235 11 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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43 Downloads (Pure)

Sustainable bioproduction of the blue pigment indigoidine: Expanding the range of heterologous products in R. toruloides to include non-ribosomal peptides

Wehrs, M., Gladden, J. M., Liu, Y., Platz, L., Prahl, J. P., Moon, J., Papa, G., Sundstrom, E., Geiselman, G. M., Tanjore, D., Keasling, J. D., Pray, T. R., Simmons, B. A. & Mukhopadhyay, A., 2019, In : Green Chemistry. 21, 12, p. 3394-3406 13 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
105 Downloads (Pure)

Synthetic Biology for Fundamental Biochemical Discovery

Budin, I. & Keasling, J. D., 2019, In : Biochemistry. 58, 11, p. 1464-1469 6 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Technical Advances to Accelerate Modular Type I Polyketide Synthase Engineering towards a Retro-biosynthetic Platform

Pang, B., Valencia, L. E., Wang, J., Wan, Y., Lal, R., Zargar, A. & Keasling, J. D., 2019, In : Biotechnology and Bioprocess Engineering. 24, 3, p. 413-423

Research output: Contribution to journalJournal articleResearchpeer-review

The transcriptome and flux profiling of Crabtree-negative hydroxy acid producing strains of Saccharomyces cerevisiae reveals changes in the central carbon metabolism

Jessop-Fabre, M. M., Dahlin, J., Biron, M. B., Stovicek, V., Ebert, B. E., Blank, L. M., Budin, I., Keasling, J. D. & Borodina, I., 2019, In : Biotechnology Journal. 14, 9, 11 p., e1900013.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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112 Downloads (Pure)
2018

A combinatorial approach to synthetic transcription factor-promoter combinations for yeast strain engineering

Dossani, Z. Y., Apel, A. R., Szmidt-Middleton, H., Hillson, N. J., Deutsch, S., Keasling, J. D. & Mukhopadhyay, A., 2018, In : Yeast. 35, 3, p. 273-280

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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472 Downloads (Pure)

A Pseudomonas putida efflux pump acts on short-chain alcohols

Basler, G., Thompson, M., Tullman-Ercek, D. & Keasling, J., 2018, In : Biotechnology for Biofuels. 11, 1, 10 p., 136.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
228 Downloads (Pure)

Biochemical Characterization of β-Amino Acid Incorporation in Fluvirucin B2 Biosynthesis

Barajas, J. F., Zargar, A., Pang, B., Benites, V. T., Gin, J., Baidoo, E. E. K., Petzold, C. J., Hillson, N. J. & Keasling, J. D., 2018, In : Chembiochem. 19, 13, p. 1391-1395

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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90 Downloads (Pure)

CasPER, a method for directed evolution in genomic contexts using mutagenesis and CRISPR/Cas9

Jakočiūnas, T., Pedersen, L. E., Lis, A. V., Jensen, M. K. & Keasling, J. D., 2018, In : Metabolic Engineering. 48, p. 288-296

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
494 Downloads (Pure)

ClusterCAD: a computational platform for type I modular polyketide synthase design

Eng, C. H., Backman, T. W. H., Bailey, C. B., Magnan, C., Martin, H. G., Katz, L., Baldi, P. & Keasling, J. D., 2018, In : Nucleic acids research. 46, D1, p. D509-D515

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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205 Downloads (Pure)

Constraining genome-scale models to represent the bow tie structure of metabolism for 13C metabolic flux analysis

Backman, T. W. H., Ando, D., Singh, J., Keasling, J. D. & Martín, H. G., 2018, In : Metabolites. 8, 1, 17 p., 3.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
210 Downloads (Pure)

De novo DNA synthesis using polymerasenucleotide conjugates

Palluk, S., Arlow, D. H., De Rond, T., Barthel, S., Kang, J. S., Bector, R., Baghdassarian, H. M., Truong, A. N., Kim, P. W., Singh, A. K., Hillson, N. J. & Keasling, J. D., 2018, In : Nature Biotechnology. 36, 7, p. 645-650

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access

Discovery of enzymes for toluene synthesis from anoxic microbial communities

Beller, H. R., Rodrigues, A. V., Zargar, K., Wu, Y-W., Saini, A. K., Saville, R. M., Pereira, J. H., Adams, P. D., Tringe, S. G., Petzold, C. J. & Keasling, J. D., 2018, In : Nature Chemical Biology. 14, p. 451-457

Research output: Contribution to journalJournal articleResearchpeer-review

File
123 Downloads (Pure)

Engineered Production of Short-Chain Acyl-Coenzyme A Esters in Saccharomyces cerevisiae

Krink-Koutsoubelis, N., Loechner, A. C., Lechner, A., Link, H., Denby, C. M., Vögeli, B., Erb, T. J., Yuzawa, S., Jakociunas, T., Katz, L., Jensen, M. K., Sourjik, V. & Keasling, J. D., 2018, In : A C S Synthetic Biology. 7, 4, p. 1105-1115 11 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Engineering β-oxidation in Yarrowia lipolytica for methyl ketone production

Hanko, E. K. R., Denby, C. M., Nogué, V. S. I., Lin, W., Ramirez, K. J., Singer, C. A., Beckham, G. T. & Keasling, J. D., 2018, In : Metabolic Engineering. 48, p. 52-62

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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189 Downloads (Pure)

Improving methyl ketone production in Escherichia coli by heterologous expression of NADH-dependent FabG

Goh, E. B., Chen, Y., Petzold, C. J., Keasling, J. D. & Beller, H. R., 2018, In : Biotechnology and Bioengineering. 115, 5, p. 1161-1172

Research output: Contribution to journalJournal articleResearchpeer-review

Industrial brewing yeast engineered for the production of primary flavor determinants in hopped beer

Denby, C. M., Li, R. A., Vu, V. T., Costello, Z., Lin, W., Chan, L. J. G., Williams, J., Donaldson, B., Bamforth, C. W., Petzold, C. J., Scheller, H. V., Martin, H. G. & Keasling, J. D., 2018, In : Nature Communications. 9, 965.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
391 Downloads (Pure)

Integrated analysis of isopentenyl pyrophosphate (IPP) toxicity in isoprenoid-producing Escherichia coli

George, K. W., Thompson, M., Kim, J., Baidoo, E. E. K., Wang, G., Benites, V. T., Petzold, C. J., Chan, L. J. G., Yilmaz, S., Turhanen, P., Adams, P. D., Keasling, J. D. & Lee, T. S., 2018, In : Metabolic Engineering. 47, p. 60-72 13 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
173 Downloads (Pure)

Isolation and characterization of novel mutations in the pSC101 origin that increase copy number

Thompson, M. G., Sedaghatian, N., Barajas, J. F., Wehrs, M., Bailey, C. B., Kaplan, N., Hillson, N. J., Mukhopadhyay, A. & Keasling, J. D., 2018, In : Scientific Reports. 8, 11 p., 1590.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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215 Downloads (Pure)

Modular 5′-UTR hexamers for context-independent tuning of protein expression in eukaryotes

Petersen, S. D., Zhang, J., Lee, J. S., Jakočiūnas, T., Grav, L. M., Kildegaard, H. F., Keasling, J. D. & Jensen, M. K., 2018, In : Nucleic acids research. 46, 21, 11 p., e127.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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174 Downloads (Pure)

Polyketide synthases as a platform for chemical product design

Zargar, A., Barajas, J. F., Lal, R. & Keasling, J. D., 2018, In : AIChE Journal. 64, 12, p. 4201-4207

Research output: Contribution to journalJournal articleResearchpeer-review

Probing the Flexibility of an Iterative Modular Polyketide Synthase with Non-Native Substrates in Vitro

Curran, S. C., Hagen, A., Poust, S., Chan, L. L. G., Garabedian, B. M., de Rond, T., Baluyot, M-J., Vu, J. T., Lau, A. K., Yuzawa, S., Petzold, C. J., Katz, L. & Keasling, J. D., 2018, In : A C S Chemical Biology. 13, 8, p. 2261-2268

Research output: Contribution to journalJournal articleResearchpeer-review

Production efficiency of the bacterial non-ribosomal peptide indigoidine relies on the respiratory metabolic state in S. cerevisiae

Wehrs, M., Prahl, J-P., Moon, J., Li, Y., Tanjore, D., Keasling, J. D., Pray, T. & Mukhopadhyay, A., 2018, In : Microbial Cell Factories. 17, 1, 11 p., 193.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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184 Downloads (Pure)

Renewable production of high density jet fuel precursor sesquiterpenes from Escherichia coli

Liu, C-L., Tian, T., Alonso-Gutierrez, J., Garabedian, B., Wang, S., Baidoo, E. E. K., Benites, V., Chen, Y., Petzold, C. J., Adams, P. D., Keasling, J. D., Tan, T. & Lee, T. S., 2018, In : Biotechnology for Biofuels. 11, 15 p., 285.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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205 Downloads (Pure)

Short-chain ketone production by engineered polyketide synthases in Streptomyces albus

Yuzawa, S., Mirsiaghi, M., Jocic, R., Fujii, T., Masson, F., Benites, V. T., Baidoo, E. E. K., Sundstrom, E., Tanjore, D., Pray, T. R., George, A., Davis, R. W., Gladden, J. M., Simmons, B. A., Katz, L. & Keasling, J. D., 2018, In : Nature Communications. 9, 4569.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
158 Downloads (Pure)

Synthetic biology of polyketide synthases

Yuzawa, S., Backman, T. W. H., Keasling, J. D. & Katz, L., 2018, In : Journal of Industrial Microbiology and Biotechnology. 45, 7, p. 621-633

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access

Toward industrial production of isoprenoids in Escherichia coli: Lessons learned from CRISPR-Cas9 based optimization of a chromosomally integrated mevalonate pathway

Alonso-Gutierrez, J., Koma, D., Hu, Q., Yang, Y., Chan, L. J. G., Petzold, C. J., Adams, P. D., Vickers, C. E., Nielsen, L. K., Keasling, J. D. & Lee, T. S., 2018, In : Biotechnology and Bioengineering. 115, 4, p. 1000-1013

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
118 Downloads (Pure)

Viscous control of cellular respiration by membrane lipid composition

Budin, I., Rond, T. D., Chen, Y., Chan, L. J. G., Petzold, C. J. & Keasling, J. D., 2018, In : Science. 362, 6419, p. 1186–1189

Research output: Contribution to journalJournal articleResearchpeer-review

2017

A Cas9-based toolkit to program gene expression in Saccharomyces cerevisiae

Apel, A. R., d'Espaux, L., Wehrs, M., Sachs, D., Li, R. A., Tong, G. J., Garber, M., Nnadi, O., Zhuang, W., Hillson, N. J., Keasling, J. D. & Mukhopadhyay, A., 2017, In : Nucleic acids research. 45, 1, p. 496-508

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
256 Downloads (Pure)

Application of an Acyl-CoA Ligase from Streptomyces aizunensis for Lactam Biosynthesis

Zhang, J., Barajas, J. F., Burdu, M., Wang, G., Baidoo, E. E. & Keasling, J. D., 2017, In : A C S Synthetic Biology. 6, 5, p. 884-890

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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217 Downloads (Pure)

Autonomous control of metabolic state by a quorum sensing (QS)-mediated regulator for bisabolene production in engineered E. coli

Kim, E-M., Min Woo, H., Tian, T., Yilmaz, S., Javidpour, P., Keasling, J. D. & Soon Lee, T., 2017, In : Metabolic Engineering. 44, p. 325-336

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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296 Downloads (Pure)