• Kogle Allé 6, Cederhuset, xx

    2970 Hørsholm

    Denmark

20152020

Research output per year

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Research Output

2020

Evolution-guided engineering of small-molecule biosensors

Snoek, T., Chaberski, E. K., Ambri, F., Kol, S., Bjørn, S. P., Pang, B., Barajas, J. F., Welner, D. H., Jensen, M. K. & Keasling, J. D., 2020, In : Nucleic acids research. 48, 1, e3.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
101 Downloads (Pure)

High-resolution scanning of optimal biosensor reporter promoters in yeast

Ambri, F., D'ambrosio, V., Di Blasi, R., Maury, J., Baallal Jacobsen, S. A., McCloskey, D., Jensen, M. K. & Keasling, J. D., 2020, In : ACS Synthetic Biology. 9, 2, p. 218-226

Research output: Contribution to journalJournal articleResearchpeer-review

Insights into the Mechanism of Phenylacetate Decarboxylase (PhdB), a Toluene-Producing Glycyl Radical Enzyme

Rodrigues, A. V., Tantillo, D. J., Mukhopadhyay, A., Mukhopadhyay, A., Keasling, J. & Beller, H. R., 2020, In : ChemBioChem. 21, 5, p. 663-671

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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40 Downloads (Pure)

Leveraging host metabolism for bisdemethoxycurcumin production in Pseudomonas putida

Incha, M. R., Thompson, M. G., Blake-Hedges, J. M., Liu, Y., Pearson, A. N., Schmidt, M., Gin, J. W., Petzold, C. J., Deutschbauer, A. M. & Keasling, J. D., 2020, In : Metabolic Engineering Communications. 10, e00119.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
59 Downloads (Pure)

Structural Mechanism of Regioselectivity in an Unusual Bacterial Acyl-CoA Dehydrogenase

Blake-Hedges, J. M., Pereira, J. H., Cruz-Morales, P., Thompson, M. G., Barajas, J. F., Chen, J., Krishna, R. N., Chan, L. J. G., Nimlos, D., Alonso-Martinez, C., Baidoo, E. E. K., Chen, Y., Gin, J. W., Katz, L., Petzold, C. J., Adams, P. D. & Keasling, J. D., 2020, In : Journal of the American Chemical Society. 142, 2, p. 835-846

Research output: Contribution to journalJournal articleResearchpeer-review

2019

A High-Throughput Mass Spectrometric Enzyme Activity Assay Enabling the Discovery of Cytochrome P450 Biocatalysts

de Rond, T., Gao, J., Zargar, A., de Raad, M., Cunha, J., Northen, T. R. & Keasling, J. D., 2019, In : ANGEWANDTE CHEMIE. 58, 30, p. 10114-10119 7 p.

Research output: Contribution to journalJournal articleResearchpeer-review

A rapid methods development workflow for high-throughput quantitative proteomic applications

Chen, Y., Vu, J., Thompson, M. G., Sharpless, W. A., Chan, L. J. G., Gin, J. W., Keasling, J. D., Adams, P. D. & Petzold, C. J., 2019, In : PLOS ONE. 14, 2, 16 p., e0211582.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
227 Downloads (Pure)

Automated "cells-To-Peptides" Sample Preparation Workflow for High-Throughput, Quantitative Proteomic Assays of Microbes

Chen, Y., Guenther, J. M., Gin, J. W., Chan, L. J. G., Costello, Z., Ogorzalek, T. L., Tran, H. M., Blake-Hedges, J. M., Keasling, J. D., Adams, P. D., García Martín, H., Hillson, N. J. & Petzold, C. J., 2019, In : Journal of Proteome Research. 18, 10, p. 3752-3761 10 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Building a global alliance of biofoundries

Hillson, N., Caddick, M., Cai, Y., Carrasco, J. A., Chang, M. W., Curach, N. C., Bell, D. J., Le Feuvre, R., Friedman, D. C., Fu, X., Gold, N. D., Herrgård, M. J., Holowko, M. B., Johnson, J. R., Johnson, R. A., Keasling, J. D., Kitney, R. I., Kondo, A., Liu, C., Martin, V. J. J. & 16 others, Menolascina, F., Ogino, C., Patron, N. J., Pavan, M., Poh, C. L., Pretorius, I. S., Rosser, S. J., Scrutton, N. S., Storch, M., Tekotte, H., Travnik, E., Vickers, C. E., Yew, W. S., Yuan, Y., Zhao, H. & Freemont, P. S., 2019, In : Nature Communications. 10, 2040.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
218 Downloads (Pure)

Complete biosynthesis of cannabinoids and their unnatural analogues in yeast

Luo, X., Reiter, M. A., d’Espaux, L., Wong, J., Denby, C. M., Lechner, A., Zhang, Y., Grzybowski, A. T., Harth, S., Lin, W., Lee, H., Yu, C., Shin, J., Deng, K., Benites, V. T., Wang, G., Baidoo, E. E. K., Chen, Y., Dev, I., Petzold, C. J. & 1 others, Keasling, J. D., 2019, In : Nature. 567, 7746, p. 123-126 4 p.

Research output: Contribution to journalLetterResearchpeer-review

Coupling S-adenosylmethionine-dependent methylation to growth: Design and uses

Luo, H., Hansen, A. S. L., Yang, L., Schneider, K., Kristensen, M., Christensen, U., Christensen, H. B., Du, B., Özdemir, E., Feist, A. M., Keasling, J. D., Jensen, M. K., Herrgård, M. J. & Palsson, B. O., 2019, In : PLOS Biology. 17, 3, e2007050.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
280 Downloads (Pure)

Distinct functional roles for hopanoid composition in the chemical tolerance of Zymomonas mobilis

Brenac, L., Baidoo, E. E. K., Keasling, J. D. & Budin, I., 2019, In : Molecular Microbiology. 112, 5, p. 1564-1575 12 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Engineered Reversal of Function in Glycolytic Yeast Promoters

Rajkumar, A. S., Özdemir, E., Lis, A. V., Schneider, K., Qin, J., Jensen, M. K. & Keasling, J. D., 2019, In : ACS Synthetic Biology. 8, 6, p. 1462-1468 7 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
200 Downloads (Pure)

Exploring small-scale chemostats to scale up microbial processes: 3-hydroxypropionic acid production in S. cerevisiae

Lis, A. V., Schneider, K., Weber, J., Keasling, J. D., Jensen, M. K. & Klein, T., 2019, In : Microbial Cell Factories. 18, 1, 11 p., 50.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
230 Downloads (Pure)

Liquid chromatography and mass spectrometry analysis of isoprenoid intermediates in Escherichia coli

Baidoo, E. E. K., Wang, G., Joshua, C. J., Benites, V. T. & Keasling, J. D., 2019, Microbial Metabolomics. Baidoo, E. E. K. (ed.). New York: Humana Press, Vol. 1859. p. 209-224 (Methods in Molecular Biology).

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

Massively parallel fitness profiling reveals multiple novel enzymes in Pseudomonas putida lysine metabolism

Thompson, M. G., Blake-Hedges, J. M., Cruz-Morales, P., Barajas, J. F., Curran, S. C., Eiben, C. B., Harris, N. C., Benites, V. T., Gin, J. W., Sharpless, W. A., Twigg, F. F., Skyrud, W., Krishna, R. N., Pereira, J. H., Baidoo, E. E. K., Petzold, C. J., Adams, P. D., Arkin, A. P., Deutschbauer, A. M. & Keasling, J. D., 2019, In : mBio. 10, 3, 17 p., e02577-18.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
100 Downloads (Pure)

Mevalonate Pathway Promiscuity Enables Noncanonical Terpene Production

Eiben, C. B., De Rond, T., Bloszies, C., Gin, J., Chiniquy, J., Baidoo, E. E. K., Petzold, C. J., Hillson, N. J., Fiehn, O. & Keasling, J. D., 2019, In : ACS Synthetic Biology. 8, 10, p. 2238-2247 10 p.

Research output: Contribution to journalJournal articleResearchpeer-review

File
13 Downloads (Pure)

Omics-driven identification and elimination of valerolactam catabolism in Pseudomonas putida KT2440 for increased product titer

Thompson, M. G., Valencia, L. E., Blake-Hedges, J. M., Cruz-Morales, P., Velasquez, A. E., Pearson, A. N., Sermeno, L. N., Sharpless, W. A., Benites, V. T., Chen, Y., Baidoo, E. E. K., Petzold, C. J., Deutschbauer, A. M. & Keasling, J. D., 2019, In : Metabolic Engineering Communications. 9, e00098.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access

Optimization of the IPP-bypass mevalonate pathway and fed-batch fermentation for the production of isoprenol in Escherichia coli

Kang, A., Mendez-Perez, D., Goh, E. B., Baidoo, E. E. K., Benites, V. T., Beller, H. R., Keasling, J. D., Adams, P. D., Mukhopadhyay, A. & Lee, T. S., 2019, In : Metabolic Engineering. 56, p. 85-96 12 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Robust characterization of two distinct glutarate sensing transcription factors of pseudomonas putida L -Lysine metabolism

Thompson, M. G., Costello, Z., Hummel, N. F. C., Cruz-Morales, P., Blake-Hedges, J. M., Krishna, R. N., Skyrud, W., Pearson, A. N., Incha, M. R., Shih, P. M., Garcia-Martin, H. & Keasling, J. D., 2019, In : ACS Synthetic Biology. 8, 10, p. 2385-2396

Research output: Contribution to journalJournal articleResearchpeer-review

Structural insights into dehydratase substrate selection for the borrelidin and fluvirucin polyketide synthases

Barajas, J. F., McAndrew, R. P., Thompson, M. G., Backman, T. W. H., Pang, B., de Rond, T., Pereira, J. H., Benites, V. T., Martín, H. G., Baidoo, E. E. K., Hillson, N. J., Adams, P. D. & Keasling, J. D., 2019, In : Journal of Industrial Microbiology and Biotechnology. 46, 8, p. 1225-1235 11 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
47 Downloads (Pure)

Sustainable bioproduction of the blue pigment indigoidine: Expanding the range of heterologous products in R. toruloides to include non-ribosomal peptides

Wehrs, M., Gladden, J. M., Liu, Y., Platz, L., Prahl, J. P., Moon, J., Papa, G., Sundstrom, E., Geiselman, G. M., Tanjore, D., Keasling, J. D., Pray, T. R., Simmons, B. A. & Mukhopadhyay, A., 2019, In : Green Chemistry. 21, 12, p. 3394-3406 13 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
107 Downloads (Pure)

Synthetic Biology for Fundamental Biochemical Discovery

Budin, I. & Keasling, J. D., 2019, In : Biochemistry. 58, 11, p. 1464-1469 6 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Technical Advances to Accelerate Modular Type I Polyketide Synthase Engineering towards a Retro-biosynthetic Platform

Pang, B., Valencia, L. E., Wang, J., Wan, Y., Lal, R., Zargar, A. & Keasling, J. D., 2019, In : Biotechnology and Bioprocess Engineering. 24, 3, p. 413-423

Research output: Contribution to journalJournal articleResearchpeer-review

The transcriptome and flux profiling of Crabtree-negative hydroxy acid producing strains of Saccharomyces cerevisiae reveals changes in the central carbon metabolism

Jessop-Fabre, M. M., Dahlin, J., Biron, M. B., Stovicek, V., Ebert, B. E., Blank, L. M., Budin, I., Keasling, J. D. & Borodina, I., 2019, In : Biotechnology Journal. 14, 9, 11 p., e1900013.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
112 Downloads (Pure)
2018

A combinatorial approach to synthetic transcription factor-promoter combinations for yeast strain engineering

Dossani, Z. Y., Apel, A. R., Szmidt-Middleton, H., Hillson, N. J., Deutsch, S., Keasling, J. D. & Mukhopadhyay, A., 2018, In : Yeast. 35, 3, p. 273-280

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
480 Downloads (Pure)

An Orthogonal and pH-Tunable Sensor-Selector for Muconic Acid Biosynthesis in Yeast

Snoek, T., Romero-Suarez, D., Zhang, J., Ambri, F., Skjoedt, M. L., Sudarsan, S., Jensen, M. K. & Keasling, J. D., 2018, In : A C S Synthetic Biology. 7, 4, p. 995-1003

Research output: Contribution to journalLetterResearchpeer-review

Open Access
File
53 Downloads (Pure)

A Pseudomonas putida efflux pump acts on short-chain alcohols

Basler, G., Thompson, M., Tullman-Ercek, D. & Keasling, J., 2018, In : Biotechnology for Biofuels. 11, 1, 10 p., 136.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
230 Downloads (Pure)

Assembly and Multiplex Genome Integration of Metabolic Pathways in Yeast Using CasEMBLR

Jakočiūnas, T., Jensen, E. D., Jensen, M. K. & Keasling, J. D., 2018, Synthetic Metabolic Pathways. Vol. 1671. p. 185-201 (Methods in Molecular Biology).

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

Biochemical Characterization of β-Amino Acid Incorporation in Fluvirucin B2 Biosynthesis

Barajas, J. F., Zargar, A., Pang, B., Benites, V. T., Gin, J., Baidoo, E. E. K., Petzold, C. J., Hillson, N. J. & Keasling, J. D., 2018, In : Chembiochem. 19, 13, p. 1391-1395

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
94 Downloads (Pure)

CasPER, a method for directed evolution in genomic contexts using mutagenesis and CRISPR/Cas9

Jakočiūnas, T., Pedersen, L. E., Lis, A. V., Jensen, M. K. & Keasling, J. D., 2018, In : Metabolic Engineering. 48, p. 288-296

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
502 Downloads (Pure)

ClusterCAD: a computational platform for type I modular polyketide synthase design

Eng, C. H., Backman, T. W. H., Bailey, C. B., Magnan, C., Martin, H. G., Katz, L., Baldi, P. & Keasling, J. D., 2018, In : Nucleic acids research. 46, D1, p. D509-D515

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
206 Downloads (Pure)

Commodity Chemicals From Engineered Modular Type I Polyketide Synthases

Yuzawa, S., Zargar, A., Pang, B., Katz, L. & Keasling, J. D., 2018, Methods in Enzymology: Enzymes in Synthetic Biology. Elsevier, Vol. 608. p. 393-415 (Methods in Enzymology).

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

Constraining genome-scale models to represent the bow tie structure of metabolism for 13C metabolic flux analysis

Backman, T. W. H., Ando, D., Singh, J., Keasling, J. D. & Martín, H. G., 2018, In : Metabolites. 8, 1, 17 p., 3.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
211 Downloads (Pure)

Cover Feature: Biochemical Characterization of β‐Amino Acid Incorporation in Fluvirucin B2 Biosynthesis (ChemBioChem 13/2018)

Barajas, J. F., Zargar, A., Pang, B., Benites, V. T., Gin, J., Baidoo, E. E. K., Petzold, C. J., Hillson, N. J. & Keasling, J. D., 2018

Research output: Non-textual form2D/3D (physical products)Research

De novo DNA synthesis using polymerasenucleotide conjugates

Palluk, S., Arlow, D. H., De Rond, T., Barthel, S., Kang, J. S., Bector, R., Baghdassarian, H. M., Truong, A. N., Kim, P. W., Singh, A. K., Hillson, N. J. & Keasling, J. D., 2018, In : Nature Biotechnology. 36, 7, p. 645-650

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access

Design, Engineering, and Characterization of Prokaryotic Ligand-Binding Transcriptional Activators as Biosensors in Yeast

Ambri, F., Snoek, T., Skjødt, M. L., Jensen, M. K. & Keasling, J. D., 2018, Synthetic Metabolic Pathways. Springer, Vol. 1671. p. 269-290 (Methods in Molecular Biology).

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

Discovery of enzymes for toluene synthesis from anoxic microbial communities

Beller, H. R., Rodrigues, A. V., Zargar, K., Wu, Y-W., Saini, A. K., Saville, R. M., Pereira, J. H., Adams, P. D., Tringe, S. G., Petzold, C. J. & Keasling, J. D., 2018, In : Nature Chemical Biology. 14, p. 451-457

Research output: Contribution to journalJournal articleResearchpeer-review

File
124 Downloads (Pure)

Engineered Production of Short-Chain Acyl-Coenzyme A Esters in Saccharomyces cerevisiae

Krink-Koutsoubelis, N., Loechner, A. C., Lechner, A., Link, H., Denby, C. M., Vögeli, B., Erb, T. J., Yuzawa, S., Jakociunas, T., Katz, L., Jensen, M. K., Sourjik, V. & Keasling, J. D., 2018, In : A C S Synthetic Biology. 7, 4, p. 1105-1115 11 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Engineering β-oxidation in Yarrowia lipolytica for methyl ketone production

Hanko, E. K. R., Denby, C. M., Nogué, V. S. I., Lin, W., Ramirez, K. J., Singer, C. A., Beckham, G. T. & Keasling, J. D., 2018, In : Metabolic Engineering. 48, p. 52-62

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
195 Downloads (Pure)

Improving methyl ketone production in Escherichia coli by heterologous expression of NADH-dependent FabG

Goh, E. B., Chen, Y., Petzold, C. J., Keasling, J. D. & Beller, H. R., 2018, In : Biotechnology and Bioengineering. 115, 5, p. 1161-1172

Research output: Contribution to journalJournal articleResearchpeer-review

Industrial brewing yeast engineered for the production of primary flavor determinants in hopped beer

Denby, C. M., Li, R. A., Vu, V. T., Costello, Z., Lin, W., Chan, L. J. G., Williams, J., Donaldson, B., Bamforth, C. W., Petzold, C. J., Scheller, H. V., Martin, H. G. & Keasling, J. D., 2018, In : Nature Communications. 9, 965.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
393 Downloads (Pure)

Integrated analysis of isopentenyl pyrophosphate (IPP) toxicity in isoprenoid-producing Escherichia coli

George, K. W., Thompson, M., Kim, J., Baidoo, E. E. K., Wang, G., Benites, V. T., Petzold, C. J., Chan, L. J. G., Yilmaz, S., Turhanen, P., Adams, P. D., Keasling, J. D. & Lee, T. S., 2018, In : Metabolic Engineering. 47, p. 60-72 13 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
175 Downloads (Pure)

Isolation and characterization of novel mutations in the pSC101 origin that increase copy number

Thompson, M. G., Sedaghatian, N., Barajas, J. F., Wehrs, M., Bailey, C. B., Kaplan, N., Hillson, N. J., Mukhopadhyay, A. & Keasling, J. D., 2018, In : Scientific Reports. 8, 11 p., 1590.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
216 Downloads (Pure)

Modular 5′-UTR hexamers for context-independent tuning of protein expression in eukaryotes

Petersen, S. D., Zhang, J., Lee, J. S., Jakočiūnas, T., Grav, L. M., Kildegaard, H. F., Keasling, J. D. & Jensen, M. K., 2018, In : Nucleic acids research. 46, 21, 11 p., e127.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
175 Downloads (Pure)

Polyketide synthases as a platform for chemical product design

Zargar, A., Barajas, J. F., Lal, R. & Keasling, J. D., 2018, In : AIChE Journal. 64, 12, p. 4201-4207

Research output: Contribution to journalJournal articleResearchpeer-review

Probing the Flexibility of an Iterative Modular Polyketide Synthase with Non-Native Substrates in Vitro

Curran, S. C., Hagen, A., Poust, S., Chan, L. L. G., Garabedian, B. M., de Rond, T., Baluyot, M-J., Vu, J. T., Lau, A. K., Yuzawa, S., Petzold, C. J., Katz, L. & Keasling, J. D., 2018, In : A C S Chemical Biology. 13, 8, p. 2261-2268

Research output: Contribution to journalJournal articleResearchpeer-review

Production efficiency of the bacterial non-ribosomal peptide indigoidine relies on the respiratory metabolic state in S. cerevisiae

Wehrs, M., Prahl, J-P., Moon, J., Li, Y., Tanjore, D., Keasling, J. D., Pray, T. & Mukhopadhyay, A., 2018, In : Microbial Cell Factories. 17, 1, 11 p., 193.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
184 Downloads (Pure)

Renewable production of high density jet fuel precursor sesquiterpenes from Escherichia coli

Liu, C-L., Tian, T., Alonso-Gutierrez, J., Garabedian, B., Wang, S., Baidoo, E. E. K., Benites, V., Chen, Y., Petzold, C. J., Adams, P. D., Keasling, J. D., Tan, T. & Lee, T. S., 2018, In : Biotechnology for Biofuels. 11, 15 p., 285.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
205 Downloads (Pure)