Photo of Gyun Min Lee
  • Kemitorvet, 220, 506F

    2800 Kgs. Lyngby

    Denmark

20162020

Research output per year

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Research Output

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Journal article
2020

Awakening dormant glycosyltransferases in CHO cells with CRISPRa

Julie la Cour Karottki, K., Hefzi, H., Xiong, K., Shamie, I., Hansen, A. H., Li, S., Pedersen, L. E., Li, S., Lee, J. S., Min Lee, G., Kildegaard, H. F. & Lewis, N. E., 2020, In : Biotechnology and Bioengineering. 117, 2, p. 593-598

Research output: Contribution to journalJournal articleResearchpeer-review

Knockout of sialidase and pro-apoptotic genes in Chinese hamster ovary cells enables the production of recombinant human erythropoietin in fed-batch cultures

Ha, T. K., Hansen, A. H., Kildegaard, H. F. & Lee, G. M., 2020, In : Metabolic Engineering. 57, p. 182-192

Research output: Contribution to journalJournal articleResearchpeer-review

2019

BiP Inducer X: An ER Stress Inhibitor for Enhancing Recombinant Antibody Production in CHO Cell Culture

Kwang Ha, T., Hansen, A. H., Kildegaard, H. F. & Min Lee, G., 2019, In : Biotechnology Journal. e1900130.

Research output: Contribution to journalJournal articleResearchpeer-review

Glyco-engineered CHO cell lines producing alpha-1-antitrypsin and C1 esterase inhibitor with fully humanized N-glycosylation profiles

Amann, T., Hansen, A. H., Kol, S., Hansen, H. G., Arnsdorf, J., Nallapareddy, S., Voldborg, B., Min Lee, G., Andersen, M. R. & Kildegaard, H. F., 2019, In : Metabolic Engineering. 52, p. 143-152 10 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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47 Downloads (Pure)

Improving recombinant bone morphogenetic protein-4 (BMP-4) production by autoregulatory feedback loop removal using BMP receptor-knockout CHO cell lines

Kim, C. L. & Min Lee, G., 2019, In : Metabolic Engineering. 52, p. 57-67

Research output: Contribution to journalJournal articleResearchpeer-review

Mitigating Clonal Variation in Recombinant Mammalian Cell Lines

Lee, J. S., Kildegaard, H. F., Lewis, N. E. & Min Lee, G., 2019, In : Trends in Biotechnology. 37, 9, p. 931-942 12 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Reduced apoptosis in Chinese hamster ovary cells via optimized CRISPR interference

Xiong, K., Marquart, K. F., Julie la Cour Karottki, K., Li, S., Shamie, I., Lee, J. S., Gerling, S., Yeo, N. C., Chavez, A., Min Lee, G., Lewis, N. E. & Kildegaard, H. F., 2019, In : Biotechnology and Bioengineering. 116, 7, p. 1813-1819 7 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Systematic Evaluation of Site-Specific Recombinant Gene Expression for Programmable Mammalian Cell Engineering

Pristovšek, N., Nallapareddy, S., Grav, L. M., Hefzi, H., Lewis, N. E., Rugbjerg, P., Hansen, H. G., Lee, G. M., Andersen, M. R. & Kildegaard, H. F., 2019, In : ACS Synthetic Biology. 8, 4, p. 758-774 17 p.

Research output: Contribution to journalJournal articleResearchpeer-review

2018

Baicalein reduces oxidative stress in CHO cell cultures and improves recombinant antibody productivity

Kwang Ha, T., Hansen, A. H., Kol, S., Kildegaard, H. F. & Min Lee, G., 2018, In : Biotechnology Journal. 13, 3, 10 p., 1700425.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
263 Downloads (Pure)

Comprehensive characterization of glutamine synthetase-mediated selection for the establishment of recombinant CHO cells producing monoclonal antibodies

Noh, S. M., Shin, S. & Min Lee, G., 2018, In : Scientific Reports. 8, 11 p., 5361.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
192 Downloads (Pure)

CRISPR/Cas9-multiplexed editing of Chinese hamster ovary B4Gal-T1, 2, 3 and 4 Tailors N-Glycan Profiles of Therapeutics and Secreted Host Cell Proteins

Amann, T., Hansen, A. H., Kol, S., Min Lee, G., Andersen, M. R. & Kildegaard, H. F., 2018, In : Biotechnology Journal. 13, 10, 9 p., 1800111 .

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
80 Downloads (Pure)

Minimizing Clonal Variation during Mammalian Cell Line Engineering for Improved Systems Biology Data Generation

Grav, L. M., Sergeeva, D., Lee, J. S., Marín de Mas, I., Lewis, N. E., Andersen, M. R., Nielsen, L. K., Min Lee, G. & Kildegaard, H. F., 2018, In : A C S Synthetic Biology. 7, 9, p. 2148-2159

Research output: Contribution to journalJournal articleResearchpeer-review

Revealing key determinants of clonal variation in transgene expression in recombinant CHO cells using targeted genome editing

Lee, J. S., Park, J. H., Kwang Ha, T., Samoudi, M., Lewis, N. E., Palsson, B. O., Kildegaard, H. F. & Min Lee, G., 2018, In : A C S Synthetic Biology. 7, 12, p. 2867-2878

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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3 Downloads (Pure)

Using titer and titer normalized to confluence are complementary strategies for obtaining Chinese hamster ovary cell lines with high volumetric productivity of etanercept

Pristovšek, N., Hansen, H. G., Sergeeva, D., Borth, N., Min Lee, G., Andersen, M. R. & Kildegaard, H. F., 2018, In : Biotechnology Journal. 13, 3, 10 p., 1700216.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
203 Downloads (Pure)
2017

Improving the secretory capacity of Chinese hamster ovary cells by ectopic expression of effector genes: Lessons learned and future directions

Hansen, H. G., Pristovsek, N., Kildegaard, H. F. & Min Lee, G., 2017, In : Biotechnology Advances. 35, 1, p. 64–76 35 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
1970 Downloads (Pure)

Ribosome profiling-guided depletion of an mRNA increases cell growth rate and protein secretion

Beuchert Kallehauge, T., Li, S., Pedersen, L. E., Kwang Ha, T., Ley, D., Andersen, M. R., Kildegaard, H. F., Min Lee, G. & Lewis, N. E., 2017, In : Scientific Reports. 7, 12 p., 40388.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
370 Downloads (Pure)
2016

Accelerated Homology-Directed Targeted Integration of Transgenes in Chinese Hamster Ovary Cells Via CRISPR/Cas9 and Fluorescent Enrichment

Lee, J. S., Grav, L. M., Pedersen, L. E., Min Lee, G. & Kildegaard, H. F., 2016, In : Biotechnology and Bioengineering (Print). 113, 11, p. 2518-2523 6 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Case study on human α1-antitrypsin: Recombinant protein titers obtained by commercial ELISA kits are inaccurate

Hansen, H. G., Kildegaard, H. F., Min Lee, G. & Stefan Kol, S., 2016, In : Biotechnology Journal. 11, 12, p. 1648–1656 9 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
555 Downloads (Pure)

Combinatorial treatment with lithium chloride enhances recombinant antibody production in transiently transfected CHO and HEK293E cells

Kim, C. L., Kwang Ha, T. & Min Lee, G., 2016, In : Biotechnology and Bioprocess Engineering. 21, 5, p. 667-675 9 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Endoplasmic reticulum-directed recombinant mRNA displays subcellular localization equal to endogenous mRNA during transient expression in CHO cells

Beuchert Kallehauge, T., Kol, S., Andersen, M. R., Kroun Damgaard, C., Min Lee, G. & Kildegaard, H. F., 2016, In : Biotechnology Journal. 11, 10, p. 1362-1367 8 p.

Research output: Contribution to journalJournal articleResearchpeer-review