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Research Output

1995

Isozyme hybrids within the protruding third loop domain of the barley alpha-amylase (beta/alpha)8-barrel. Implication for BASI sensitivity and substrate affinity

Juge, N., Rodenburg, K. W., Guo, X-J., Chaix, J-C. & Svensson, B., 1995, In : F E B S Letters. 363, p. 299-303

Research output: Contribution to journalJournal articleResearchpeer-review

Mutational analysis of the roles in catalysis and substrate recognition of arginines 54 and 305, aspartic acid 309, and tryptophan 317 located at subsites 1 and 2 in glucoamylase from Aspergillus niger

Frandsen, T. P., Christensen, T., Stoffer, B., Lehmbeck, J., Dupont, C., Honzatko, R. B. & Svensson, B., 1995, In : Biochemistry. 34, 32, p. 10162-10169

Research output: Contribution to journalJournal articleResearchpeer-review

Novel disaccharides containing sulfur in the ring and nitrogen in the interglycosidic linkage. Conformation of methyl 5'-thio-4-N-maltoside bound to glucoamylase and its activity as a competitive inhibitor

Andrews, J. S., Weimar, T., Frandsen, T. P., Svensson, B. & Pinto, B. M., 1995, In : Journal of the American Chemical Society. 117, p. 10799-10804

Research output: Contribution to journalJournal articleResearchpeer-review

Refined structure for the complex of D-gluco-dihydroacarbose with glucoamylase from Aspergillus awamori var. X100 to 2.2 A resolution: dual conformations for extended inhibitors bound to the active site of glucoamylase

Stoffer, B., Aleshin, A. E., Firsov, L. M., Svensson, B. & Honzatko, R. B., 1995, In : F E B S Letters. 358, p. 57-61

Research output: Contribution to journalJournal articleResearchpeer-review

Stopped-flow kinetic studies of the reaction of barley alpha-amylase/subtilisin inhibitor and the high pI barley alpha-amylasev

Sidenius, U., Olsen, K., Svensson, B. & Christensen, U., 1995, In : F E B S Letters. 361, p. 250-254

Research output: Contribution to journalJournal articleResearchpeer-review

Synthesis and enzymatic activity of novel heteroanalogues of methyl maltoside containing sulfur and selenium

Metha, S., Andrews, J. S., Johnston, B. D., Svensson, B. & Pinto, B. M., 1995, In : Journal of the American Chemical Society. 117, p. 9783-9790

Research output: Contribution to journalJournal articleResearchpeer-review

Thermodynamics of inhibitor binding to mutant forms of glucoamylase from Aspergillus niger determined by isothermal titration calorimetry

Berland, C. R., Sigurskjold, B. W., Stoffer, B., Frandsen, T. P. & Svensson, B., 1995, In : Biochemistry. 34, 32, p. 10153-10161

Research output: Contribution to journalJournal articleResearchpeer-review

1994

Characterization, crystallization and preliminary X-ray crystallographic analysis of the complex between barley alpha-amylase and the bifunctional alpha-amylase/subtilisin inhibitor from barley seeds

Vallée, F., Kadziola, A., Bourne, Y., Abe, J., Svensson, B. & Haser, R., 1994, In : Journal of Molecular Biology. 236, p. 368-371

Research output: Contribution to journalJournal articleResearchpeer-review

Crystal and molecular structure of barley alpha-amylase

Kadziola, A., Abe, J., Svensson, B. & Haser, R., 1994, In : Journal of Molecular Biology. 239, p. 104-121

Research output: Contribution to journalJournal articleResearchpeer-review

Domain B protruding at the third beta strand of the alpha/beta barrel in barley alpha-amylase confers distinct isozyme-specific properties

Rodenburg, K. W., Juge, N., Guo, X-J., Søgaard, M., Chaix, J-C. & Svensson, B., 1994, In : European Journal of Biochemistry. 221, p. 277-284

Research output: Contribution to journalJournal articleResearchpeer-review

Minireview. Protein engineering in the alpha-amylase family: Catalytic mechanism, substrate specificity, and stability

Svensson, B., 1994, In : Plant Molecular Biology. 25, 2, p. 141-157

Research output: Contribution to journalJournal articleResearchpeer-review

NMR spectroscopy of exchangeable protons of glucoamylase and of complexes with inhibitors in the 9-15-ppm range

Firsov, L. M., Neustroev, K. N., Aleshin, A. E., Metzler, C. M., Metzler, D. E., Scott, R. D., Stoffer, B., Christensen, T. & Svensson, B., 1994, In : European Journal of Biochemistry. 223, p. 293-303

Research output: Contribution to journalJournal articleResearchpeer-review

Production of active, insect-specific scorpion neurotoxin in yeast

Martin-Eauclaire, M-F., Søgaard, M., Ramos, C. A., Cestèle, S., Bougis, P. E. & Svensson, B., 1994, In : European Journal of Biochemistry. 223, p. 637-645

Research output: Contribution to journalJournal articleResearchpeer-review

Protein engineering of the relative specificity of glucoamylase from Aspergillus awamori based on sequence similarities between starch-degrading enzymes

Sierks, M. R. & Svensson, B., 1994, In : Protein Engineering. 7, p. 1479-1484

Research output: Contribution to journalJournal articleResearchpeer-review

Site-directed mutagenesis of the catalytic base glutamic acid 400 in glucoamylase from Aspergillus niger and of tyrosine 48 and glutamine 401, both hydrogen-bonded to the gamma-carboxylate group of glutamic acid 400

Frandsen, T. P., Dupont, C., Lehmbeck, J., Stoffer, B., Sierks, M. R., Honzatko, R. B. & Svensson, B., 1994, In : Biochemistry. 33, 46, p. 13808-13816

Research output: Contribution to journalJournal articleResearchpeer-review

Thermodynamics of inhibitor binding to the catalytic site of glucoamylase from Aspergillus niger determined by displacement titration calorimetry

Sigurskjold, B. W., Berland, C. R. & Svensson, B., 1994, In : Biochemistry. 33, 33, p. 10191-10199

Research output: Contribution to journalJournal articleResearchpeer-review

Thermodynamics of ligand binding to the starch-binding domain of glucoamylase from Aspergillus niger

Sigurskjold, B. W., Svensson, B., Williamson, G. & Driguez, H., 1994, In : European Journal of Biochemistry. 225, p. 133-141

Research output: Contribution to journalJournal articleResearchpeer-review

1993

Alpha-Amylases: Structure and function

Søgaard, M., Abe, J., Martin-Eauclaire, M-F. & Svensson, B., 1993, In : Carbohydrate Polymers. 21, p. 137-146

Research output: Contribution to journalConference articleResearchpeer-review

Arginine is essential for the alpha-amylase inhibitory activity of the alpha-amylase/subtilisin inhibitor (BASI) from barley seeds

Abe, J., Sidenius, U. & Svensson, B., 1993, In : Biochemical Journal. 293, p. 151-155

Research output: Contribution to journalJournal articleResearchpeer-review

Comparison of barley malt alpha-amylase isozymes 1 and 2: construction of cDNA hybrids by in vivo recombination and their expression in yeast

Juge, N., Søgaard, M., Chaix, J-C., Martin-Eauclaire, M-F., Svensson, B., Marchis-Mouren, G. & Guo, X-J., 1993, In : Gene. 130, p. 159-166

Research output: Contribution to journalJournal articleResearchpeer-review

Electrospray mass spectrometry characterization of post-translational modifications of barley alpha-amylase 1 produced in yeast

Søgaard, M., Andersen, J. S., Roepsdorff, P. & Svensson, B., 1993, In : BIO-TECHNOLOGY. 11, 10, p. 1162-1165

Research output: Contribution to journalJournal articleResearchpeer-review

Functional roles and subsite locations of Leu177, Trp178 and Asn182 of Aspergillus awamori glucoamylase determined by site-directed mutagenesis

Sierks, M. R., Ford, C., Reilly, P. J. & Svensson, B., 1993, In : Protein Engineering. 6, p. 75-79

Research output: Contribution to journalJournal articleResearchpeer-review

Functional roles of the invariant aspartic acid 55, tyrosine 306, and aspartic acid 309 in glucoamylase from Aspergillus awamori studied by mutagenesis

Sierks, M. R. & Svensson, B., 1993, In : Biochemistry. 32, 4, p. 1113-1117

Research output: Contribution to journalJournal articleResearchpeer-review

Mutational analysis of glycosylase function: A mini-review

Svensson, B. & Søgaard, M., 1993, In : Journal of Biotechnology. 29, p. 1-37

Research output: Contribution to journalJournal articleResearchpeer-review

Phosphorylation of barley and wheat phospholipid transfer proteins by wheat calcium-dependent protein kinase

Neumann, G. M., Condron, R., Svensson, B. & Polya, G. M., 1993, In : Plant Science. 92, 2, p. 159-167

Research output: Contribution to journalJournal articleResearchpeer-review

Production, purification and characterization of the catalytic domain of glucoamylase from Aspergillus niger

Stoffer, B., Frandsen, T. P., Busk, P. K., Svendsen, I., Schneider, P. & Svensson, B., 1993, In : Biochemical Journal. 292, p. 197-202

Research output: Contribution to journalJournal articleResearchpeer-review

Reaction mechanisms of Trp120-->Phe and wild-type glucoamylases from Aspergillus niger. Interactions with maltooligodextrins and acarbose

Olsen, K., Christensen, U., Sierks, M. R. & Svensson, B., 1993, In : Biochemistry. 32, 37, p. 9686-9693

Research output: Contribution to journalJournal articleResearchpeer-review

Starch- and glycogen-debranching and branching enzymes: prediction of structural features of the catalytic (beta/alpha)8-barrel domain and evolutionary relationship to other amylolytic enzymes

Jespersen, H. M., MacGregor, E. A., Henrissat, B., Sierks, M. R. & Svensson, B., 1993, In : Journal of Protein Chemistry. 12, p. 791-805

Research output: Contribution to journalJournal articleResearchpeer-review

1992

Active site similarities of glucose dehydrogenase, glucose oxidase, and glucoamylase probed by deoxygenated substrates

Sierks, M. R., Bock, K., Refn, S. & Svensson, B., 1992, In : Biochemistry. 31, 37, p. 8972-8977

Research output: Contribution to journalJournal articleResearchpeer-review

Barley malt-alpha-amylase. Purification, action pattern, and subsite mapping of isozyme 1 and two members of the isozyme 2 subfamily using p-nitrophenylated maltooligosaccharide substrates

Ajandouz, E. H., Abe, J., Svensson, B. & Marchis-Mouren, G., 1992, In : BBA General Subjects. 1159, p. 193-202

Research output: Contribution to journalJournal articleResearchpeer-review

Kinetic identification of a hydrogen bonding pair in the glucoamylase-maltose transition state complex

Sierks, M. R. & Svensson, B., 1992, In : Protein Engineering. 5, p. 185-188

Research output: Contribution to journalJournal articleResearchpeer-review

Primary structure of barwin: a barley seed protein closely related to the C-terminal domain of proteins encoded by wound-induced plant genes

Svensson, B., Svendsen, I., Høyrup, P., Roepstorff, P., Ludvigsen, S. & Poulsen, F. M., 1992, In : Biochemistry. 31, 37, p. 8767-8770

Research output: Contribution to journalJournal articleResearchpeer-review

Protein engineering of amylases

Svensson, B. & Søgaard, M., 1992, In : Biochemical Society. Transactions. 20, 1, p. 34-42

Research output: Contribution to journalConference articleResearchpeer-review

Stopped-flow fluorescence and steady-state kinetic studies of ligand-binding reactions of glucoamylase from Aspergillus niger

Olsen, K., Svensson, B. & Christensen, U., 1992, In : European Journal of Biochemistry. 209, p. 777-784

Research output: Contribution to journalJournal articleResearchpeer-review

Synthesis of 6'-S-alpha-D-glucopyranosyl-6'-thiomaltose (6(2)-thiopanose) and its effect on the enzymatic activity of glucoamylases from Aspergillus niger

Cottaz, S., Sierks, M. R. & Svensson, B., 1992, In : Carbohydrate Research. 228, 1, p. 299-305

Research output: Contribution to journalJournal articleResearchpeer-review

1991

Comparison of the domain-level organization of starch hydrolases and related enzymes

Jespersen, H. M., MacGregor, E. A., Sierks, M. R. & Svensson, B., 1991, In : Biochemical Journal. 280, p. 51-55

Research output: Contribution to journalJournal articleResearchpeer-review

C-terminal processing of barley alpha-amylase 1 in malt, aleurone protoplasts, and yeast

Søgaard, M., Olsen, F. L. & Svensson, B., 1991, In : Proc Natl Acad Sci U S A. 88, p. 8140-8144

Research output: Contribution to journalJournal articleResearchpeer-review

Effect of limited proteolysis in the 8th loop of the barrel and of antibodies on porcine pancreas amylase activity

Desseaux, V., Payan, F., Ajandouz, E. H., Svensson, B., Haser, R. & Marchis-Mouren, G., 1991, In : BBA General Subjects. 1080, p. 237-244

Research output: Contribution to journalJournal articleResearchpeer-review

Limited proteolysis of porcine pancreatic and barley isozyme 2 alpha-amylases occurs in specific loops of their (beta/alpla)8-barrel domain

Desseaux, V., Svensson, B., Payan, F., Haser, R. & Marchis-Mouren, G., 1991, In : Food Hydrocolloids. 5, p. 209-213

Research output: Contribution to journalJournal articleResearchpeer-review

Structure/function relationships in amylases

Svensson, B., Sierks, M. R., Jespersen, H. & Søgaard, M., 1991, ACS Symposium Series 458: Biotechnology of Amylodextrin Oligosaccharides. Friedman, R. (ed.). p. 28-43

Research output: Chapter in Book/Report/Conference proceedingArticle in proceedingsResearchpeer-review

Structure/function relationships in starch-hydrolases and related enzymes

Svensson, B., 1991, In : Denpun Kagaku. 38, p. 125-135

Research output: Contribution to journalConference articleResearchpeer-review

1990

Activity and thermal stability of genetically truncated forms of Aspergillus glucoamylase

Evans, R., Ford, C., Sierks, M. R., Nikolov, Z. & Svensson, B., 1990, In : Gene. 91, p. 131-134

Research output: Contribution to journalJournal articleResearchpeer-review

Catalytic mechanism of fungal glucoamylase as defined by mutagenesis of Asp176, Glu179 and Glu180 in the enzyme from Aspergillus awamori

Sierks, M. R., Ford, C., Reilly, P. J. & Svensson, B., 1990, In : Protein Engineering. 3, p. 193-198

Research output: Contribution to journalJournal articleResearchpeer-review

Elucidation of the functional roles of active site residues in Aspergillus niger glucoamylase by chemical modification and mutagenesis

Svensson, B., Sierks, M. R., Ford, C. & Reilly, P. J., 1990, Protein Engineering. Protein Design in Basic Research, Medicine and Industry. Ikehara, M., Oshima, T. & Titani, K. (eds.). Japan Scientific Society & Springer Verlag, p. 137-139

Research output: Chapter in Book/Report/Conference proceedingArticle in proceedingsResearchpeer-review

Expression of cDNAs encoding barley alpha-amylase 1 and 2 in yeast and characterization of the secreted proteins

Søgaard, M. & Svensson, B., 1990, In : Gene. 94, p. 173-179

Research output: Contribution to journalJournal articleResearchpeer-review

Identification of carboxylic acid residues in glucoamylase G2 from Aspergillus niger that participate in catalysis and substrate binding

Svensson, B., Clarke, A. J., Svendsen, I. & Møller, H., 1990, In : European Journal of Biochemistry. 188, p. 29-38

Research output: Contribution to journalJournal articleResearchpeer-review

1989

A 10 kD barley basic protein transfers phosphatidylcholine from liposomes to mitochondria

Breu, V., Guerbette, F., Kader, J-C., Kannangara, G., Svensson, B. & Wettstein-Knowles, P. V., 1989, In : Carlsberg Research Communications. 54, 2, p. 81-84

Research output: Contribution to journalJournal articleResearchpeer-review