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Research Output

2000

Determination of the solution conformation of D-gluco-dihydroacarbose, a high-affinity inhibitor bound to glucoamylase by transferred NOE NMR spectroscopy

Weimar, T., Petersen, B. O., Svensson, B. & Pinto, B. M., 2000, In : Carbohydrate Research. 326, p. 50-55

Research output: Contribution to journalJournal articleResearchpeer-review

Energetic and mechanistic studies of glucoamylase using molecular recognition of maltose OH groups coupled with site-directed mutagenesis

Sierks, M. R. & Svensson, B., 2000, In : Biochemistry. 39, 29, p. 8585-8592

Research output: Contribution to journalJournal articleResearchpeer-review

Glucoamylase: structure/function relationships, and protein engineering

Sauer, J., Sigurskjold, B. W., Christensen, U., Frandsen, T. P., Mirgorodskaya, E., Harrison, M., Roepsdorff, P. & Svensson, B., 2000, In : BBA General Subjects. 1543, 2, p. 275-293

Research output: Contribution to journalJournal articleResearchpeer-review

Identification of key amino acid residues in Neisseria polysaccharea amylosucrase

Sarcabal, P., Remaud-Simeon, M., Willemot, R-M., Potocki de Montalk, G., Svensson, B. & Monsan, P., 2000, In : F E B S Letters. 474, p. 33-37

Research output: Contribution to journalJournal articleResearchpeer-review

Involvement of Gln937 of Streptococcus downei GTF-I glucansucrase in transition-state stabilization

Monchois, V., Vignon, M., Escalier, P-C., Svensson, B. & Russell, R. R. B., 2000, In : European Journal of Biochemistry. 267, p. 4127-4136

Research output: Contribution to journalJournal articleResearchpeer-review

Location of repeat elements in glucansucrases of Leuconostoc and Streptococcus species

Janecek, S., Svensson, B. & Russell, R. R. B., 2000, In : F E M S Microbiology Letters. 192, p. 53-57

Research output: Contribution to journalJournal articleResearchpeer-review

Purification, enzymatic characterization, and nucleotide sequence of a high-isoelectric-point alpha-glucosidase from barley malt

Frandsen, T. P., Lok, F., Migorodskaya, E., Roepsdorff, P. & Svensson, B., 2000, In : Plant Physiology. 123, 1, p. 275-286

Research output: Contribution to journalJournal articleResearchpeer-review

Specific inhibition of barley alpha-amylase 2 by barley alpha-amylase/subtilisin inhibitor depends on charge interactions and can be conferred to isozyme 1 by mutation

Rodenburg, K. W., Vallée, F., Juge, N., Aghajari, N., Guo, X., Haser, R. & Svensson, B., 2000, In : European Journal of Biochemistry. 267, p. 1019-1029

Research output: Contribution to journalJournal articleResearchpeer-review

Structure, specificity and function of cyclomaltodextrinase, a multispecific enzyme of the alpha-amylase family

Park, K-H., Kim, T-J., Cheong, T. K., Kim, J-W., Oh, B-H. & Svensson, B., 2000, In : BBA General Subjects. 1478, p. 165-185

Research output: Contribution to journalJournal articleResearchpeer-review

1999

alpha-1,4-glucan lyases producing 1,5-anhydro-D-fructose from starch and glycogen have sequence similarity to alpha-glucosidases

Yu, S., Bojsen, K., Svensson, B. & Marcussen, J., 1999, In : BBA General Subjects. 1433, 1-2, p. 1-15

Research output: Contribution to journalJournal articleResearchpeer-review

Dynamic light scattering evidence of a ligand-induced motion between the two domains of glucoamylase G1 of Aspergillus niger with heterobivalent substrate analogues

Payre, N., Cottaz, S., Boisset, C., Borsali, R., Svensson, B., Henrissat, B. & Driguez, H., 1999, In : Angewandte Chemie. 38, p. 974-977

Research output: Contribution to journalJournal articleResearchpeer-review

Engineering specificity and stability in glucoamylase from Aspergillus nige

Frandsen, T. P., Fierobe, H-P. & Svensson, B., 1999, Protein Engineering in Industrial Biotechnology. Alberghina, L. (ed.). Harwood Academic Publ.

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

Identification and micro sequence analysis of proteins extracted from barley seeds

Ostergaard, O. & Svensson, B., 1999, Proceedings of The 2nd European Symposium on Enzymes in Grain Processing ESEPG-2. Simoinen, T. & Tenkanen, M. (eds.). p. 223-231

Research output: Chapter in Book/Report/Conference proceedingArticle in proceedingsResearchpeer-review

Isolation and characterization of the gene encoding the starch debranching enzyme limit dextrinase from germinating barley

Kristensen, M., Lok, F., Planchot, V., Svendsen, I., Leah, R. & Svensson, B., 1999, In : BBA General Subjects. 1431, 2, p. 538-546

Research output: Contribution to journalJournal articleResearchpeer-review

Mass spectrometric identification of a stable catalytic cysteinesulfinic acid residue in an enzymatically active chemically modified glucoamylase mutant

Mirgorodskaya, E., Fierobe, H-P., Svensson, B. & Roepsdorff, P., 1999, In : Journal of Mass Spectroscopy. 34, p. 952-957

Research output: Contribution to journalJournal articleResearchpeer-review

Structure-function relationships in glucoamylases encoded by variant Saccharomycopsis fibuligera genes

Solovicova, A., Christensen, T., Hostinova, E., Gasperik, J., Sevcik, J. & Svensson, B., 1999, In : European Journal of Biochemistry. 264, p. 756-764

Research output: Contribution to journalJournal articleResearchpeer-review

Studies on structure, function, and protein engineering of starch-degrading enzymes

Svensson, B., Sass Bak-Jensen, K., Jensen, M. T., Sauer, J., Gottschalk, T. E. & Rodenburg, K. W., 1999, In : J. Appl. Glycosi.. 46, p. 49-63

Research output: Contribution to journalJournal articleResearchpeer-review

Synthesis and glycosidase inhibitory activity of 5-thioglucopyranosylamines. Molecular modeling of complexes with glucoamylase

Randall, K. D., Frandsen, T. P., Stoffer, B. B., Johnson, M. A., Svensson, B. & Pinto, B. M., 1999, In : Carbohydrate Research. 321, p. 143-156

Research output: Contribution to journalJournal articleResearchpeer-review

The engineering of specificity and stability in selected starch degrading enzymes

Svensson, B., Bak-Jensen, K. S., Mori, H., Sauer, J., Jensen, M. T., Kramhøft, B., Gottschalk, T. E., Christensen, T., Sigurskjold, B. W., Aghajari, N., Haser, R., Payre, N., Cottaz, S. & Driguez, H., 1999, Recent Advances in Carbohydrate Bioengineering. Gilbert, H. J., Davies, G. J., Henrissat, B. & Svensson, B. (eds.). Royal Society of Chemistry, p. 272-281

Research output: Chapter in Book/Report/Conference proceedingArticle in proceedingsResearchpeer-review

Thermodynamics of reversible and irreversible unfolding and domain interactions of glucoamylase from Aspergillus niger studied by differential scanning and isothermal titration calorimetry

Christensen, T., Svensson, B. & Sigurskjold, B. W., 1999, In : Biochemistry. 38, 19, p. 6300-6307

Research output: Contribution to journalJournal articleResearchpeer-review

1998

Barley alpha-amylase bound to its endogenous protein inhibitor BASI: crystal structure of the complex at 1.9 A resolution

Vallée, F., Kadziola, A., Bourne, Y., Juy, M., Rodenburg, K. W., Svensson, B. & Haser, R., 1998, In : Structure. 6, 5, p. 649-659

Research output: Contribution to journalJournal articleResearch

Binding site mutants of glucoamylase from Aspergillus niger with decreased Km towards C-6 substituted isomaltosides

Frandsen, T. P., Palcic, M. M., Dupont, C. & Svensson, B., 1998, In : Carbohydrate Research. 314, p. 127-133

Research output: Contribution to journalJournal articleResearchpeer-review

Effect of temperature and Ca2+ on the degree of multiple attack exhibited by mesophilic and thermophilic alpha-amylases

Kramhøft, B. & Svensson, B., 1998, Stability and Stabilisation of Biocatalysts. Ballesteros, A., Plou, F. J., Iborra, J. L. & Halling, P. J. (eds.). Elsevier, p. 343-347 (Progress in Biotechnology; No. 15).

Research output: Chapter in Book/Report/Conference proceedingArticle in proceedingsResearchpeer-review

Enzymatic properties of the cysteinesulfinic acid derivative of the catalytic-base mutant Glu400 -> Cys of glucoamylase from Aspergillus awamori

Fierobe, H-P., Clarke, A. J., Tull, D. & Svensson, B., 1998, In : Biochemistry. 37, 11, p. 3753-3759

Research output: Contribution to journalJournal articleResearchpeer-review

Large-scale purification and characterization of barley limit dextrinase, a member of the alpha-amylase structural family

Kristensen, M., Planchot, V., Abe, J. & Svensson, B., 1998, In : Cereal Chemistry. 75, 4, p. 473-479

Research output: Contribution to journalJournal articleResearchpeer-review

Molecular structure of a barley alpha-amylase-inhibitor complex: implications for starch binding and catalysis

Kadziola, A., Søgaard, M., Svensson, B. & Haser, R., 1998, In : Journal of Molecular Biology. 278, p. 205-217

Research output: Contribution to journalJournal articleResearchpeer-review

Restoration of catalytic activity beyond wild-type level in glucoamylase from Aspergillus awamori by oxidation of the Glu400-->Cys catalytic-base mutant to cysteinesulfinic acid

Fierobe, H-P., Mirgorodskaya, E., MacGuire, K. A., Roepsdorff, P., Svensson, B. & Clarke, A. J., 1998, In : Biochemistry. 37, 11, p. 3743-3752

Research output: Contribution to journalJournal articleResearchpeer-review

Secretion, purification, and characterisation of barley alpha-amylase produced by heterologous gene expression in Aspergillus niger

Juge, N., Svensson, B. & Williamson, G., 1998, In : Applied Microbiology and Biotechnology. 49, 4, p. 385-392

Research output: Contribution to journalJournal articleResearchpeer-review

Structure, function, and protein engineering of starch-degrading enzymes

Gottschalk, T. E., Fierobe, H-P., Mirgorodskaya, E., Clarke, A. J., Tull, D., Sigurskjold, B. W., Christensen, T., Payre, N., Frandsen, T. P., Juge, N., MacGuire, K. A., Cottaz, S., Roepsdorff, P., Driguez, H., Williamson, G. & Svensson, B., 1998, In : Biochemical Society. Transactions. 26, 2, p. 198-204

Research output: Contribution to journalConference articleResearchpeer-review

1997

Domain evolution in the alpha-amylase family

Janecek, S., Svensson, B. & Henrissat, B., 1997, In : Journal of Molecular Evolution. 45, p. 322-331

Research output: Contribution to journalJournal articleResearchpeer-review

Glucoamylase mutants in the conserved active-site segment Trp170-Tyr175 located at a distance from the site of catalysis

Stoffer, B. B., Dupont, C., Frandsen, T. P., Lehmbeck, J. & Svensson, B., 1997, In : Protein Eng.. 10, p. 81-87

Research output: Contribution to journalJournal articleResearchpeer-review

Some details of the reaction mechanism of glucoamylase from Aspergillus niger--kinetic and structural studies on Trp52-->Phe and Trp317-->Phe mutants

Christensen, T., Stoffer, B. B., Svensson, B. & Christensen, U., 1997, In : European Journal of Biochemistry. 250, p. 638-645

Research output: Contribution to journalJournal articleResearchpeer-review

1996

A circularly permuted alpha-amylase-type alpha/beta-barrel structure in glucan-synthesizing glucosyltransferases

MacGregor, E. A., Jespersen, H. M. & Svensson, B., 1996, In : F E B S Letters. 378, p. 263-266

Research output: Contribution to journalJournal articleResearchpeer-review

Catalytic mechanism of glucoamylase probed by mutagenesis in conjunction with hydrolysis of alpha-D-glucopyranosyl fluoride and maltooligosaccharides

Sierks, M. R. & Svensson, B., 1996, In : Biochemistry. 35, 6, p. 1865-1871

Research output: Contribution to journalJournal articleResearchpeer-review

Chemical mapping of the active site of the glucoamylase from Aspergillus niger

Lemieux, R. U., Spohr, U., Bach, M., Cameron, D. R., Frandsen, T. P., Stoffer, B. B., Svensson, B. & Palcic, M. M., 1996, In : Canadian Journal of Chemistry. 74, 3, p. 319-335

Research output: Contribution to journalJournal articleResearchpeer-review

Crystallographic complexes of glucoamylase with maltooligosaccharide analogs: relationship of stereochemical distortions at the nonreducing end to the catalytic mechanism

Aleshin, A. E., Stoffer, B. B., Firsov, L. M., Svensson, B. & Honzatko, R. B., 1996, In : Biochemistry. 35, 25, p. 8319-8328

Research output: Contribution to journalJournal articleResearchpeer-review

Large scale purification and partial primary structure of limit dextrinase

Planchot, V., Kristensen, M., Abe, J., Svendsen, I. & Svensson, B., 1996, Proceedings of the First European Symposium on Enzymes in Grain Processing. van Hartingsveldt, W., Heidekamp, F. & Hamer, R. (eds.). p. 87-94

Research output: Chapter in Book/Report/Conference proceedingArticle in proceedingsResearchpeer-review

Mutational modulation of substrate bond-type specificity and thermostability of glucoamylase from Aspergillus awamori by replacement with short homologue active site sequences and thiol/disulfide engineering

Fierobe, H-P., Stoffer, B. B., Frandsen, T. P. & Svensson, B., 1996, In : Biochemistry. 35, 26, p. 8696-8704

Research output: Contribution to journalJournal articleResearch

Overexpression, purification, and characterization of recombinant barley alpha-amylases 1 and 2 secreted by the methylotrophic yeast Pichia pastoris

Juge, N., Andersen, J. S., Tull, D., Roepstorff, P. & Svensson, B., 1996, In : Protein Expr. Purif.. 8, p. 204-214

Research output: Contribution to journalJournal articleResearchpeer-review

Protein engineering investigations of structure-function relationships in barley alpha-amylase and the inhibition by barley alpha-amylase/subtilisin inhibitor

Svensson, B., Matsui, I., Gottschalk, T., Juge, N., Tull, D. & Rodenburg, K. W., 1996, Proceedings of the First European Symposium on Enzymes in Grain Processing. van Hartingsveldt, W., Heidekamp, F. & Hamer, R. (eds.). 222 p.

Research output: Chapter in Book/Report/Conference proceedingArticle in proceedingsResearchpeer-review

Structure and energetics of the glucoamylase-isomaltose transition-state complex probed by using modeling and deoxygenated substrates coupled with site-directed mutagenesis

Frandsen, T. P., Stoffer, B. B., Palcic, M. M., Hof, S. & Svensson, B., 1996, In : Journal of Molecular Biology. 26, p. 79-89

Research output: Contribution to journalJournal articleResearchpeer-review

Substrate binding mechanism of Glu180->Gln, Asp176->Asn, and wild-type glucoamylases from Aspergillus niger

Christensen, U., Olsen, K., Stoffer, B. B. & Svensson, B., 1996, In : Biochemistry. 35, 47, p. 15009-15018

Research output: Contribution to journalJournal articleResearchpeer-review

1995

Arg-27, Arg-127 and Arg-155 in the beta-trefoil protein barley alpha-amylase/subtilisin inhibitor are interface residues in the complex with barley alpha-amylase 2

Rodenburg, K. W., Várallyay, E., Svendsen, I. & Svensson, B., 1995, In : Biochemical Journal. 309, p. 969-976

Research output: Contribution to journalJournal articleResearchpeer-review

Characteristic differences in the primary structure allow discrimination of cyclodextrin glucanotransferases from alpha-amylases

Janecek, S., MacGregor, E. A. & Svensson, B., 1995, In : Biochemical Journal. 305, p. 685-687

Research output: Contribution to journalJournal articleResearchpeer-review

Chemoenzymatic synthesis of 6 omega-S-alpha-D-glucopyranosyl-6 omega-thiomaltooligosaccharides: their binding to Aspergillus niger glucoamylase G1 and its starch-binding domain

Apperu, C., Driguez, H., Williamson, G. & Svensson, B., 1995, In : Carbohydrate Research. 277, 2, p. 313-320

Research output: Contribution to journalJournal articleResearchpeer-review