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Research Output

2004

Proteome analysis of barley seeds: Identification of major proteins from two-dimensional gels (pl 4-7)

Østergaard, O., Finnie, C., Laugesen, S., Roepstorff, P. & Svensson, B., 2004, In : Proteomics. 4, 8, p. 2437-2447

Research output: Contribution to journalJournal articleResearchpeer-review

Purification and characterization of recombinant high pI Barley α-Glucosidase

Næsted, H., Bojsen, K. & Svensson, B., 2004.

Research output: Contribution to conferencePosterResearch

Two-dimensional gel electrophoresis pattern (pH 6-11) and identification of water-soluble barley seed and malt proteins by mass spectrometry

Bak-Jensen, K. S., Laugesen, S., Roepstorff, P. & Svensson, B., 2004, In : Proteomics. 4, 3, p. 728-742

Research output: Contribution to journalJournal articleResearchpeer-review

Tyrosine 105 and threonine 212 at outermost substrate binding subsites -6 and +4 control substrate specificity, oligosaccharide cleavage patterns, and multiple binding modes of barley alpha-amylase 1

Bak-Jensen, K. S., André, G., Gottschalk, T. E., Paes, G., Tran, V. & Svensson, B., 2004, In : Journal of Biological Chemistry. 279, p. 10093-10102

Research output: Contribution to journalJournal articleResearchpeer-review

2003

: The interaction of barley -amylase and barley alpha-amylase/subtilisin inhibitor and other alpha-amylases with proteinaceous inhibitors of cereal origin

Nielsen, P. K., Bønsager, B. C., Kim, T-J., Finnie, C. & Svensson, B., 2003, Recent Advances in Enzymes in Grain Processing. Courtin, C. M., Veraverbeke, W. S. & Delcour, J. A. (eds.). Leuven, p. 173-178

Research output: Chapter in Book/Report/Conference proceedingArticle in proceedingsResearchpeer-review

A tissue-specific approach to barley proteome analysis: aleurone layer, endosperm, embryo and single seeds

Finnie, C. & Svensson, B., 2003, In : Journal of Cereal Science. 38, p. 217-227

Research output: Contribution to journalJournal articleResearchpeer-review

Barley preoteome analysis, starch degrading enzymes and proteinaceous inhibitors

Finnie, C., Østergaard, O., Bak-Jensen, K. S., Nielsen, P. K., Bønsager, B. C., Mori, H., Nøhr, J., Kramhøft, B., Juge, N. & Svensson, B., 2003, In : J. Appl. Glycosci.. 50, 277-282

Research output: Contribution to journalJournal articleResearchpeer-review

Barley -amylase isozyme 3D structures at work reveal remarkable differences and a novel role of their C-terminal domain

Robert, X., Haser, R., Svensson, B. & Agahajari, N., 2003, Recent Advances in Enzymes in Grain Processing. Courtin, C. M., Veraverbeke, W. S. & Delcour, J. A. (eds.). Leuven, p. 15-20

Research output: Chapter in Book/Report/Conference proceedingArticle in proceedingsResearchpeer-review

Chemoenzymatic synthesis of branched oligo- and polysaccharides as potential substrates for starch active enzymes

Greffe, L., Jensen, M. T., Bosso, C., Svensson, B. & Driguez, H., 2003, In : ChemBioChem. 4, 12, p. 100-104

Research output: Contribution to journalJournal articleResearchpeer-review

Cross-inhibitory activity of cereal protein inhibitors against alpha-amylases and xylanases

Sancho, A. I., Faulds, C. B., Svensson, B., Bartolomé, B., Williamson, G. & Juge, N., 2003, In : BBA General Subjects. 1650, 1-2, p. 136-144

Research output: Contribution to journalJournal articleResearchpeer-review

Differences in conformational stability of barley -amylase isozymes 1 and 2: Role of charged groups and isozyme 2 specific salt-bridges

Jensen, M. T., Gottschalk, T. E. & Svensson, B., 2003, In : Journal of Cereal Science. 38, p. 289-300

Research output: Contribution to journalJournal articleResearchpeer-review

Docking exploration of alpha-(1,6) branched maltodextrins in barley alpha-amylase

André, G., Jensen, M. T., Svensson, B. & Tran, V., 2003, Recent Advances in Enzymes in Grain Processing. Courtin, C. M., Veraverbeke, W. S. & Delcour, J. A. (eds.). Leuven, p. 9-14

Research output: Chapter in Book/Report/Conference proceedingArticle in proceedingsResearchpeer-review

Engineering of barley alpha-amylase

Bonzonnet, S., Kim, T-J., Bønsager, B., Kramhøft, B., Nielsen, P. K., Bak-Jensen, K. S. & Svensson, B., 2003, In : Biocatalysis and Biotransformation. 21, 4-5, p. 209-214

Research output: Contribution to journalConference articleResearchpeer-review

Enhanced amylolytic activity in germinating barley through synthesis of a bacterial alpha-amylase

Tull, D., Phillipson, B. A., Kramhøft, B., Knudsen, S., Olsen, O. & Svensson, B., 2003, In : Journal of Cereal Science. 37, p. 71-80

Research output: Contribution to journalJournal articleResearchpeer-review

Identification, cloning and characterization of two thioredoxin h isoforms, HvTrxh1 and HvTrxh2, from the barley seed proteome

Maeda, K., Finnie, C., Østergaard, O. & Svensson, B., 2003, In : European Journal of Biochemistry. 270, p. 2633-2643

Research output: Contribution to journalJournal articleResearchpeer-review

Impact on substrate specificity of mutational subsite isozyme mimicry in barley -amylase

Svensson, B., Mori, H., Bak-Jensen, K. S. & Jensen, M. T., 2003, In : J. Appl. Glycosci.. 50, p. 143-145

Research output: Contribution to journalJournal articleResearchpeer-review

Kinetics and energetics of the binding between barley alpha-amylase/subtilisin inhibitor and barley alpha-amylase 2 analyzed by surface plasmon resonance and isothermal titration calorimetry

Nielsen, P. K., Bønsager, B. C., Berland, C. R., Sigurskjold, B. W. & Svensson, B., 2003, In : Biochemistry. 42, 6, p. 1478-1487

Research output: Contribution to journalJournal articleResearchpeer-review

On the mechanism of alpha-amylase

Oudjeriouat, N., Moreau, Y., Santimone, M., Svensson, B., Marchis-Mouren, G. & Desseaux, V., 2003, In : European Journal of Biochemistry. 270, p. 3871-3879

Research output: Contribution to journalJournal articleResearchpeer-review

Purification and characterization of the beta-trefoil fold protein barley alpha-amylase/subtilisin inhibitor overexpressed in Escherichia coli

Bønsager, B. C., Prætorius-Ibba, M., Nielsen, P. K. & Svensson, B., 2003, In : Protein Expression and Purification. 30, 2, p. 185-193

Research output: Contribution to journalJournal articleResearchpeer-review

Relation between domain evolution, specificity, and taxonomy of the alpha-amylase family members containing a C-terminal starch-binding domain

Janecek, S., Svensson, B. & MacGregor, E. A., 2003, In : European Journal of Biochemistry. 270, p. 635-645

Research output: Contribution to journalJournal articleResearchpeer-review

Selection of a high-energy bioactive conformation of a sulfonium-ion glycosidase inhibitor by the enzyme glucoamylase G2

Johnson, M. A., Jensen, M. T., Svensson, B. & Pinto, B. M., 2003, In : Journal of the American Chemical Society. 125, p. 5663-5670

Research output: Contribution to journalJournal articleResearchpeer-review

Chemoenzymatic syntheses of linear and branched hemithiomaltodextrins as potential inhibitors for starch-debranching enzymes

Greffe, L., Jensen, M. T., Chang-Pi-Hen, F., Fruchard, S., O'Donohue, M. J., Svensson, B. & Driguez, H., 2002, In : Chemistry. 8, p. 5447-5455

Research output: Contribution to journalJournal articleResearchpeer-review

Comparison of crystal structures of crystallographic studies of barley alpha-amylase 1 and 2: implications for isozyme differences in stability and activity

Robert, X., Haser, R., Svensson, B. & Aghajari, N., 2002, In : Biologia. 57, suppl. 11, p. 59-70

Research output: Contribution to journalConference articleResearchpeer-review

Expression, purification and preliminary crystallographic studies of alpha-amylase isozyme 1 from barley seeds

Robert, X., Gottschalk, T. E., Haser, R., Svensson, B. & Aghajari, N., 2002, In : Acta Crystallographica. Section D: Biological Crystallography. 58, p. 683-686

Research output: Contribution to journalJournal articleResearchpeer-review

Fascinating facets of function and structure of amylolytic enzymes of glycoside hydrolase family

Svensson, B., Jensen, M. T., Mori, H., Bak-Jensen, K. S., Bønsager, B., Nielsen, P. K., Prætorius-Ibba, M., Nøhr, J., Juge, N., Greffe, L., Williamson, G. & Driguez, H., 2002, In : Biologia. 57, suppl. 11, p. 1-9

Research output: Contribution to journalConference articleResearchpeer-review

Inhibition of catalytic and glucan-binding activities of a streptococcal GTF forming insoluble glucans

Wright, W. G., Therwell, C., Svensson, B. & Russell, R. R. B., 2002, In : Caries Research. 36, p. 353-359

Research output: Contribution to journalJournal articleResearchpeer-review

Initial proteome analysis of mature barley seeds and malt

Østergaard, O., Melchior, S., Roepsdorff, P. & Svensson, B., 2002, In : Proteomics. 2, 6, p. 733-739

Research output: Contribution to journalJournal articleResearchpeer-review

Physicochemical characterisation of the two active site mutants Trp(52)-->Phe and Asp(55)-->Val of glucoamylase from Aspergillus niger

Christensen, T., Frandsen, T. P., Kaarsholm, N. C., Svensson, B. & Sigurskjold, B. W., 2002, In : BBA General Subjects. 1601, p. 163-171

Research output: Contribution to journalJournal articleResearchpeer-review

Proteolysis during the isoelectric focusing step of two-dimensional gel electrophoresis may be a common problem

Finnie, C. & Svensson, B., 2002, In : Analytical Biochemistry. 311, 2, p. 182-186

Research output: Contribution to journalJournal articleResearchpeer-review

Proteome analysis of grain filling and seed maturation in barley

Finnie, C., Melchior, S., Roepsdorff, P. & Svensson, B., 2002, In : Plant Physiology. 129, 3, p. 1308-1319

Research output: Contribution to journalJournal articleResearchpeer-review

Substrate recognition by three family 13 yeast alpha-glucosidases

Frandsen, T. P., Palcic, M. M. & Svensson, B., 2002, In : European Journal of Biochemistry. 269, p. 728-734

Research output: Contribution to journalJournal articleResearchpeer-review

Synthesis of 1,4-anhydro-D-xylitol heteroanalogues of the naturally-occurring glycosidase inhibitor salacinol and their evaluation as glycosidase inhibitors

Ghavami, A., Johnston, B. D., Maddess, M. D., Chinapoo, S. M., Jensen, M. T., Svensson, B. & Pinto, B. M., 2002, In : Canadian Journal of Chemistry. 80, 8, p. 932-947

Research output: Contribution to journalJournal articleResearchpeer-review

Synthesis of selenium analogues of the naturally occurring glycosidase inhibitor salacinol and their evaluation as glycosidase inhibitors

Johnston, B. D., Ghavami, A., Jensen, M. T., Svensson, B. & Pinto, B. M., 2002, In : Journal of the American Chemical Society. 124, p. 8245-8250

Research output: Contribution to journalJournal articleResearchpeer-review

The starch binding domain of glucoamylase from Aspergillus niger: overview of its structure, function, and role in raw-starch-hydrolysis

Juge, N., Le Gal-Coëffet, M-F., Furniss, C. S. M., Gunning, A. P., Kramhøft, B., Morris, V. J., Svensson, B. & Williamson, G., 2002, In : Biologia. 57, suppl. 11, p. 239-245

Research output: Contribution to journalConference articleResearchpeer-review

The structure of barley alpha-amylase isozyme 1 reveals a novel role of domain C in substrate recognition and binding: a pair of sugar tongs

Robert, X., Haser, R., Gottschalk, T. E., Ratajczek, F., Driguez, H., Svensson, B. & Aghajari, N., 2002, In : Structure. 11, p. 973-984

Research output: Contribution to journalJournal articleResearchpeer-review

2001

(Gluco)amylases, what have we learned so far?

Svensson, B., Sauer, J., Mori, H., Jensen, M. T., Bak-Jensen, K. S., Kramhøft, B., Juge, N., Nøhr, J., Greffe, L., Frandsen, T. P., Palcic, M. M., Williamson, G. & Driguez, H., 2001, Recent Advances in Carbohydrate Bioengineering. Teeri, T. T., Gilbert, H. J., Feizi, T. & Svensson, B. (eds.). Royal Society of Chemistry, p. 67-75

Research output: Chapter in Book/Report/Conference proceedingArticle in proceedingsResearchpeer-review

Both binding sites of the starch-binding domain of Aspergillus niger glucoamylase are essential for inducing a conformational change in amylose

Giardina, T., Gunning, A. P., Juge, N., Faulds, C. B., Furniss, C. S. M., Svensson, B., Morris, V. J. & Williamson, G., 2001, In : Journal of Molecular Biology. 313, p. 1149-1159

Research output: Contribution to journalJournal articleResearchpeer-review

depolymerisation and biosynthesis of polysaccharides

Stone, B. A. & Svensson, B., 2001, Glycoscience: Chemistry and Chemical Biology. Fraser-Reid, B., Tatsuta, K. & Thiem, J. (eds.). Berlin/Heidelberg: Springer Verlag, p. 1905-1990

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

Extensive N-glycosylation reduces the thermal stability of a recombinant alkalophilic bacillus alpha-amylase produced in Pichia pastoris

Tull, D., Gottschalk, T. E., Svendsen, I., Kramhøft, B., Philipson, B. A., Bisgård-Frantzen, H., Olsen, O. & Svensson, B., 2001, In : Protein Expression and Purification. 21, 1, p. 13-23

Research output: Contribution to journalJournal articleResearchpeer-review

Modulation of activity and substrate binding modes by mutation of single and double subsites +1/+2 and -5/-6 of barley alpha-amylase 1

Mori, H., Bak-Jensen, K. S., Gottschalk, T. E., Motowia, M. S., Damager, I., Møller, B. L. & Svensson, B., 2001, In : European Journal of Biochemistry. 268, p. 6545-65558

Research output: Contribution to journalJournal articleResearchpeer-review

Modulation of the multisubstrate specificity of Thermus maltogenic amylase by truncation of the N-terminal domain and by a salt-induced shift of the monomer/dimer equilibrium

Kim, T-J., Nguyen, V. D., Lee, H-S., Kim, J-W., Oh, B-H., Lee, S-B., Svensson, B. & Park, K-H., 2001, In : Biochemistry. 40, 47, p. 14182-14190

Research output: Contribution to journalJournal articleResearchpeer-review

Relationship of sequence and structure to specificity in the alpha-amylase family of enzymes

MacGregor, E. A., Janecek, S. & Svensson, B., 2001, In : BBA General Subjects. 1546, p. 1-20

Research output: Contribution to journalJournal articleResearchpeer-review

Specificity modulation of barley alpha-amylase through biased random mutagenesis involving a conserved tripeptide in beta --> alpha loop 7 of the catalytic (beta/alpha)(8)-barrel domain

Gottschalk, T. E., Tull, D., Aghajari, N., Haser, R. & Svensson, B., 2001, In : Biochemistry. 40, 43, p. 12844-12854

Research output: Contribution to journalJournal articleResearchpeer-review

Stability and function of interdomain linker variants of glucoamylase 1 from Aspergillus niger

Sauer, J., Christensen, T., Sigurskjold, B. W., Frandsen, T. P., Mirgorodskaya, E., MacGuire, K. A., Roepsdorff, P., Driguez, H. & Svensson, B., 2001, In : Biochemistry. 40, 31, p. 9336-9246

Research output: Contribution to journalJournal articleResearchpeer-review

Synthesis of an 1’-azasugar analogue of maltose

Andreasen, V., Svensson, B. & Bols, M., 2001, In : Synthesis. 2, p. 339-342

Research output: Contribution to journalJournal articleResearchpeer-review

Synthesis of nitrogen analogues of salacinol and their evaluation as glycosidase inhibitors

Ghavami, A., Johnston, B. D., Jensen, M. T., Svensson, B. & Pinto, B. M., 2001, In : Journal of the American Chemical Society. 123, p. 6268-6271

Research output: Contribution to journalJournal articleResearchpeer-review

Use of immobilised glucoamylase G2 for separation of enantiomers

Karlsson, A., Arfwidsson, I., Husovic, Z. & Svensson, B., 2001, In : Chromatographia. 54, p. 717-723

Research output: Contribution to journalJournal articleResearchpeer-review

2000

Complexes of glucoamylase with maltoside heteroanalogues: bound ligand conformations by use of transferred NOE experiments and molecular modeling

Weimar, T., Stoffer, B., Svensson, B. & Pinto, B. M., 2000, In : Biochemistry. 39, 2, p. 300-306

Research output: Contribution to journalJournal articleResearchpeer-review