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Research Output

2007

Probing the role of α-glucosidase (GH31) in the endosperm of germinating barley (Hordeum vulgare)

Stanley, D., Rejzek, H., Næsted, H., Dedola, S., Svensson, B., Field, R. A., Denyer, K. & Smith, A., 2007.

Research output: Contribution to conferenceConference abstract for conferenceResearch

Production of recombinant barley limit dextrinase

Vester-Christensen, M. B., Næsted, H. & Svensson, B., 2007.

Research output: Contribution to conferenceConference abstract for conferenceResearch

Proteome analysis of barley seed tissues for insight into germination

Finnie, C., Shahpiri, A., Bønsager, B. C., Hynek, R., Jensen, O. N., Roepstorff, P. & Svensson, B., 2007.

Research output: Contribution to conferenceConference abstract for conferenceResearch

Proteomic insights in to the developmental biology of wheat seeds

Shelton, D., Zhang, X., Jacobsen, S., Søndergaard, I., Roepstorff, P. & Svensson, B., 2007.

Research output: Contribution to conferenceConference abstract for conferenceResearch

Proteomics and protein folding in developing wheat seeds

Shelton, D., Zhang, X., Søndergaard, I., Jacobsen, S. & Svensson, B., 2007.

Research output: Contribution to conferenceConference abstract for conferenceResearch

Proteomics and protein folding in developing wheat seeds

Shelton, D., Zhang, X., Jacobsen, S., Søndergaard, I., Roepstorff, P. & Svensson, B., 2007.

Research output: Contribution to conferenceConference abstract for conferenceResearch

Roles of multiple surface sites, long substrate binding clefts, and carbohydrate binding modules in the action of amulolytic enzymes on polysaccharide substrates

Svensson, B., Nielsen, M. M., Seo, E-S., Dilokpimol, A., Andersen, J., Abou Hachem, M., Næsted, H., Willemoës, S., Bozonnet, S., Kandra, L., Gyemant, G., Aghajari, N. & Haser, R., 2007.

Research output: Contribution to conferenceConference abstract for conferenceResearch

Spatio-temporal changes in germination and radical elongation of barley seeds tracked by proteome analysis of dissected embryo, aleurone layer and endosperm tissues

Bønsager, B. C., Finnie, C., Roepstorff, P. & Svensson, B., 2007, In : Proteomics. 7, p. 4528-4540

Research output: Contribution to journalJournal articleResearchpeer-review

Spatio-temporal profiling and degradation of alpha-amylase isozymes during barley seed germination

Bak-Jensen, K. S., Laugesen, S., Østergaard, O., Finnie, C., Roepstorff, P. & Svensson, B., 2007, In : FEBS Journal. 274, 10, p. 2552-2565

Research output: Contribution to journalJournal articleResearchpeer-review

Spatio-temporal profiling and degradation of α-amylase isozymes during barley seed germination

Bak-Jensen, K. S., Laugesen, S., Østergaard, O., Finnie, C., Roepstorff, P. & Svensson, B., 2007, In : F E B S Journal. 274, p. 2552-2565

Research output: Contribution to journalJournal articleResearchpeer-review

Structural basis for substrate protein recognition by thioredoxin

Maeda, K., Hägglund, P., Henriksen, A. & Svensson, B., 2007.

Research output: Contribution to conferenceConference abstract for conferenceResearchpeer-review

Structural basis for target protein recognition by thioredoxin and quantitative cereal disulfide proteomics

Hägglund, P., Maeda, K., Finnie, C., Henriksen, A. & Svensson, B., 2007.

Research output: Contribution to conferenceConference abstract for conferenceResearch

The “pair of sugar tongs” site on the non-catalytic domain C of barley alpha-amylase participates in substrate binding and activity

Bozonnet, S., Jensen, M. T., Nielsen, M. M., Aghajari, N., Jensen, M. H., Kramhøft, B., Willemoës, M., Tranier, S., Haser, R. & Svensson, B., 2007, In : F E B S Journal. 274, p. 5055-5067

Research output: Contribution to journalJournal articleResearchpeer-review

2006

A Kunitz-type cysteine protease inhibitor from cauliflower and Arabidopsis

Halls, C. E., Rogers, S. W., Ouffattole, M., Østergaard, O., Svensson, B. & Rogers, J. C., 2006, In : Plant Science. 170, 6, p. 1102-1110

Research output: Contribution to journalJournal articleResearch

Differential appearance of isoforms and cultivar variation in protein temporal profiles revealed in the maturing barley grain proteome

Finnie, C., Bak-Jensen, K. S., Laugesen, S., Roepstorff, P. & Svensson, B., 2006, In : Plant Science. 170, 4, p. 808-821

Research output: Contribution to journalJournal articleResearchpeer-review

Enrichment and identification of integral membrane proteins from barley aleurone layers by reversed-phase chromatography, SDS-PAGE and LC-MS/MS

Hynek, R., Svensson, B., Nørregaard Jensen, O., Barkholt, V. & Finnie, C., 2006, In : Journal of Proteome Research. 5, p. 3105-3113

Research output: Contribution to journalJournal articleResearchpeer-review

Interactions of barley alpha-amylase isozymes with Ca2+, substrates and proteinaceous inhibitors

Abou Hachem, M., Bozonnet, S., Willemoes, M., Kramhoft, B., Fukuda, K., Bonsager, B. C., Jensen, M. T., Nohr, J., Tranier, S., Juge, N., Robert, X., Haser, R., Aghajari, N. & Svensson, B., 2006, In : Biocatalysis and Biotransformation. 24, 1-2, p. 83-93

Research output: Contribution to journalJournal articleResearchpeer-review

Mapping of barley alpha-amylases and outer subsite mutants reveals dynamic high-affinity subsites and barriers in the long substrate binding cleft

Kandra, L., Abou Hachem, M., Gyemant, G., Kramhoft, B. & Svensson, B., 2006, In : FEBS Letters. 580, 21, p. 5049-5053

Research output: Contribution to journalJournal articleResearchpeer-review

Mapping of barley alpha-amylases and outer subsite mutants reveals high-affinity subsites and barriers in the long substrate binding cleft

Kandra, L., Abou Hachem, M., Gyémánt, G., Kramhøft, B. & Svensson, B., 2006, In : F E B S Letters. 580, p. 5046-5053

Research output: Contribution to journalJournal articleResearchpeer-review

Production of enzymatically active recombinant full-length barley high pI alpha-glucosidase of glycoside family 31 by high cell-density fermentation of Pichia pastoris and affinity purification

Næsted, H., Kramhøft, B., Lok, F., Bojsen, K., Yu, S. & Svensson, B., 2006, In : Protein Expression and Purification. 46, 1, p. 56-63

Research output: Contribution to journalJournal articleResearchpeer-review

Proteinaceous inhibitors of carbohydrate-active enzymes in cereals - implication in agriculture, cereal-processing and nutrition

Juge, N. & Svensson, B., 2006, In : Journal of the Science of Food and Agriculture. 86, 11, p. 1573-1586

Research output: Contribution to journalJournal articleResearchpeer-review

Proteomics of disulfide and cysteine oxidoreduction.

Maeda, K., Hägglund, K. P., Finnie, C. & Svensson, B., 2006, Plant Proteomics. Finnie, C. (ed.). Oxford, UK: Blackwell Publishing, p. 71-97

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

Proteomics of Disulphide and Cysteine Oxidoreduction

Maeda, K., Hägglund, P., Finnie, C. & Svensson, B., 2006, Plant Proteomics. Finnie, C. (ed.). Oxford: Blackwell Publishing, Vol. Chapter 4. 272 p. (Annual Plant Reviews).

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearch

Structural basis for target protein recognition by the protein disulfide reductase thioredoxin

Maeda, K., Hägglund, P., Finnie, C., Svensson, B. & Henriksen, A., 2006, In : Structure. 14, 11, p. 1701-1710

Research output: Contribution to journalJournal articleResearchpeer-review

The activity of barley alpha-amylase on starch granules is enhanced by fusion of a starch binding domain from Aspergillus niger glucoamylase

Juge, N., Nøhr, J., Le Gal-Coëffet, M-F., Kramhøft, B., Furniss, C. S. M., Planchot, V., Archer, D. B., Williamson, G. & Svensson, B., 2006, In : B B A - Proteins and Proteomics. 1764, 2, p. 275-284

Research output: Contribution to journalJournal articleResearchpeer-review

2005

5-Thio-D-glycopyranosylamines and their amidinium salts as potential transition-state mimics of glycosyl hydrolases: synthesis, enzyme inhibitory activities, X-ray crystallography, and molecular modeling

Kavlekar, L. M., Kuntz, D. A., Wen, X., Johnston, B. D., Svensson, B., Rose, D. R. & Pinto, B. M., 2005, In : Tetrahedron: Asymmetry. 16, 5, p. 1035-1046

Research output: Contribution to journalJournal articleResearchpeer-review

AFM images of complexes between amylose and Aspergillus niger glucoamylase mutants, native and mutant starch binding domains: a model for the action of glucoamylase

Morris, V. M., Gunning, A. P., Faults, C. B., Williamson, G. & Svensson, B., 2005, In : Starch. 57, 1, p. 1-7

Research output: Contribution to journalJournal articleResearchpeer-review

A new clan of CBM families based on bioinformatics of starch-binding domains from families CBM20 and CBM21

Marhovic, M., Svensson, B., MacGregor, E. A. & Janecek, S., 2005, In : F E B S Journal. 272, 21, p. 5497-5523

Research output: Contribution to journalJournal articleResearchpeer-review

Biased mutagenesis in the N-terminal region by degenerate oligonucleotide gene shuffling enhances secretory expression of barley alpha-amylase 2 in yeast

Fukuda, K., Jensen, M. H., Aghajari, N., Haser, R. & Svensson, B., Nov 2005, In : Protein Engineering Design and Selection (Print). 18, 11, p. 515-526

Research output: Contribution to journalJournal articleResearchpeer-review

Binding of carbohydrates and protein inhibitors to the surface of alpha-amylases

Bozonnet, S., Bønsager, B. C., Kramhoft, B., Mori, H., Abou Hachem, M., Willemoes, M., Jensen, M. T., Fukuda, K., Nielsen, P. K., Juge, N., Aghajari, N., Tranier, S., Robert, X., Haser, R. & Svensson, B., 2005, In : Biologia. 60, p. 27-36

Research output: Contribution to journalJournal articleResearchpeer-review

Chemical synthesis of a dual branched malto-decaose: A potential substrate for alpha-amylases

Damager, I., Jensen, M., Olsen, C. E., Blennow, A., Møller, B. L., Svensson, B. & Motawia, S., Jul 2005, In : ChemBioChem. 6, 7, p. 1224-1233

Research output: Contribution to journalJournal articleResearchpeer-review

Identification of thioredoxin h-reducible disulphides in proteornes by differential labelling of cysteines: Insight into recognition and regulation of proteins in barley seeds by thioredoxin h

Maeda, K., Finnie, C. & Svensson, B., 2005, In : Proteomics. 5, 6, p. 1634-1644

Research output: Contribution to journalJournal articleResearchpeer-review

Insights into the "pair of sugar tongs" surface binding site in barley alpha-amylase isozymes and crystallization of appropriate sugar tongs mutants

Tranier, S., Deville, K., Robert, X., Bozonnet, S., Haser, R., Svensson, B. & Aghajari, N., 2005, In : Biologia. 60, p. 37-46

Research output: Contribution to journalJournal articleResearchpeer-review

Involvement of individual subsites and secondary substrate binding sites in multiple attack on amylose by barley alpha-amylase

Kramhøft, B., Bak-Jensen, K. S., Mori, H., Juge, N., Nøhr, J. & Svensson, B., 2005, In : Biochemistry. 44, 6, p. 1824-1832

Research output: Contribution to journalJournal articleResearchpeer-review

Mutational analysis of target enzyme recognition of the beta-trefoil fold barley alpha-amylase/subtilisin inhibitor

Bønsager, B. C., Nielsen, P. K., Abou Hachem, M., Fukuda, K., Praetorius-Ibba, M. & Svensson, B., 2005, In : Journal of Biological Chemistry. 280, 15, p. 14855-14864

Research output: Contribution to journalJournal articleResearchpeer-review

Mutational analysis of the β-trefoil fold protein barley α-amylase/subtilisin inhibitor probes hot spots for the interaction with barley α-amylase

Bønsager, B. C., Nielsen, P. K., Abou Hachem, M., Prætorius-Ibba, M. & Svensson, B., 2005, In : Journal of Biological Chemistry. 280, p. 14855-14864

Research output: Contribution to journalJournal articleResearchpeer-review

Oligosaccharide binding to barley alpha-amylase 1

Robert, X., Haser, R., Mori, H., Svensson, B. & Aghajari, N., 2005, In : Journal of Biological Chemistry. 280, p. 32968-32978

Research output: Contribution to journalJournal articleResearchpeer-review

Opportunities, challenges, and responsibilities of women in biochemistry in Europe

Svensson, B., 2005, Proceedings 21st COE Marine Bio-Manipulation Frontier in Food Production. Graduate School of Fisheries Sciences, Hokkaido University

Research output: Chapter in Book/Report/Conference proceedingArticle in proceedingsCommunication

Probing the structure of glucan lyases – the lytic members of GH31 - by sequence analysis, circular dichroism and proteolysis

Ernst, H., Lo Leggio, L., Yu, S., Finnie, C., Svensson, B. & Larsen, S., 2005, In : Biologia. 60, suppl 16, p. 149-159

Research output: Contribution to journalJournal articleResearchpeer-review

Structure and target-specificity of thioredoxin h

Maeda, K., Henriksen, A., Hagglund, P., Finnie, C. & Svensson, B., 2005, In : Journal of Biotechnology. 118, p. S19-S19

Research output: Contribution to journalConference abstract in journalResearchpeer-review

2004

Aspects of the barley seed proteome during development and germination

Finnie, C., Maeda, K., Østergaard, O., Bak-Jensen, K. S., Larsen, J. & Svensson, B., 2004, In : Biochemical Society. Transactions. 32, p. 517-519

Research output: Contribution to journalJournal articleResearchpeer-review

Barley alpha-amylase/subtilisin inhibitor: structure, biophysics and protein engineering

Nielsen, P. K., Bønsager, B. C., Fukuda, K. & Svensson, B., 2004, In : BBA General Subjects. 1696, p. 157-164

Research output: Contribution to journalJournal articleResearchpeer-review

Characterization of recombinant high pI Barley α-Glucosidase

Næsted, H. & Svensson, B., 2004.

Research output: Contribution to conferencePosterResearch

Cy5 maleimide labelling for sensitive detection of free thiols in native protein extracts: identification of seed proteins targeted by barley thioredoxin h isoforms

Maeda, K., Finnie, C. & Svensson, B., 2004, In : Biochemical Journal. 378, p. 497-507

Research output: Contribution to journalJournal articleResearchpeer-review

Environmental and transgene expression effects on the barley seed proteome

Finnie, C., Steenholdt, T., Noguera, O. R., Knudsen, S., Larsen, J., Brinch-Pedersen, H., Holm, P. B., Olsen, O. & Svensson, B., 2004, In : Phytochemistry. 65, 11, p. 1619-1627

Research output: Contribution to journalJournal articleResearchpeer-review

Potential physiological role of plant glycosidase inhibitors

Bellincampi, D., Carmadella, L., Delcour, J. A., Desseaux, V., D'Ovidio, R., A., D., Elliot, G., Gebruers, K., Giovane, A., Juge, N., Sørensen, J. F., Svensson, B. & Vairo, D., 2004, In : BBA General Subjects. 1696, p. 265-274

Research output: Contribution to journalJournal articleResearchpeer-review

Potential role of glycosidase inhibitors in industrial biotechnological applications

Sørensen, J. F., Kragh, K. M., Sibbesen, O., Delcour, J., Goesaert, H., Svensson, B., Tahir, T. A., Brufau, J., Perez-Vendrell, A. M., Bellincampi, D., D'Ovidio, R., Camardella, L., Giovane, A., Bonnin, E. & Juge, N., 2004, In : BBA General Subjects. 1696, p. 275-287

Research output: Contribution to journalJournal articleResearchpeer-review

PRODUCTION OF RECOMBINANT HIGH pI-BARLEY α-GLUCOSIDASE

Næsted, H. & Svensson, B., 2004.

Research output: Contribution to conferencePosterResearch

Proteinaceous alpha-araylase inhibitors.

Svensson, B., Fukuda, K., Nielsen, P. K. & Bønsager, B. C., 2004, In : BBA General Subjects. 1696, p. 145-156

Research output: Contribution to journalJournal articleResearchpeer-review