Research Output 2013 2020

Filter
Journal article
2020

Awakening dormant glycosyltransferases in CHO cells with CRISPRa

Julie la Cour Karottki, K., Hefzi, H., Xiong, K., Shamie, I., Hansen, A. H., Li, S., Pedersen, L. E., Li, S., Lee, J. S., Min Lee, G., Kildegaard, H. F. & Lewis, N. E., 2020, In : Biotechnology and Bioengineering. 117, 2, p. 593-598

Research output: Contribution to journalJournal articleResearchpeer-review

Knockout of sialidase and pro-apoptotic genes in Chinese hamster ovary cells enables the production of recombinant human erythropoietin in fed-batch cultures

Ha, T. K., Hansen, A. H., Kildegaard, H. F. & Lee, G. M., 2020, In : Metabolic Engineering. 57, p. 182-192

Research output: Contribution to journalJournal articleResearchpeer-review

2019

BCAT1 and BCAT2 disruption in CHO cells has cell line-dependent effects

Pereira, S., Ley, D., Schubert, M., Grav, L. M., Kildegaard, H. F. & Andersen, M. R., 2019, In : Journal of Biotechnology. 306, p. 24-31 8 p.

Research output: Contribution to journalJournal articleResearchpeer-review

BiP Inducer X: An ER Stress Inhibitor for Enhancing Recombinant Antibody Production in CHO Cell Culture

Kwang Ha, T., Hansen, A. H., Kildegaard, H. F. & Min Lee, G., 2019, In : Biotechnology Journal. e1900130.

Research output: Contribution to journalJournal articleResearchpeer-review

Genetic engineering approaches to improve post-translational modification of biopharmaceuticals in different production platforms

Amann, T., Schmieder, V., Kildegaard, H. F., Borth, N. & Andersen, M. R., 2019, In : Biotechnology and Bioengineering. 116, 10, p. 2778-2796

Research output: Contribution to journalJournal articleResearchpeer-review

87 Downloads (Pure)

Genome-wide systematic identification of methyltransferase recognition and modification patterns

Jensen, T. Ø., Tellgren-Roth, C., Redl, S. M. A., Maury, J., Baallal Jacobsen, S. A., Pedersen, L. E. & Nielsen, A. T., 2019, In : Nature Communications. 10, 1, 3311.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
47 Downloads (Pure)
Open Access
File

Improving recombinant bone morphogenetic protein-4 (BMP-4) production by autoregulatory feedback loop removal using BMP receptor-knockout CHO cell lines

Kim, C. L. & Min Lee, G., 2019, In : Metabolic Engineering. 52, p. 57-67

Research output: Contribution to journalJournal articleResearchpeer-review

Mitigating Clonal Variation in Recombinant Mammalian Cell Lines

Lee, J. S., Kildegaard, H. F., Lewis, N. E. & Min Lee, G., 2019, In : Trends in Biotechnology. 37, 9, p. 931-942 12 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Reduced apoptosis in Chinese hamster ovary cells via optimized CRISPR interference

Xiong, K., Marquart, K. F., Julie la Cour Karottki, K., Li, S., Shamie, I., Lee, J. S., Gerling, S., Yeo, N. C., Chavez, A., Min Lee, G., Lewis, N. E. & Kildegaard, H. F., 2019, In : Biotechnology and Bioengineering. 116, 7, p. 1813-1819 7 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Reprogramming AA catabolism in CHO cells with CRISPR/Cas9 genome editing improves cell growth and reduces byproduct secretion

Ley, D., Pereira, S., Pedersen, L. E., Arnsdorf, J., Hefzi, H., Davy, A. M., Kwang Ha, T., Wulff, T., Kildegaard, H. F. & Andersen, M. R., 2019, In : Metabolic Engineering. 56, p. 120-129 10 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Systematic Evaluation of Site-Specific Recombinant Gene Expression for Programmable Mammalian Cell Engineering

Pristovšek, N., Nallapareddy, S., Grav, L. M., Hefzi, H., Lewis, N. E., Rugbjerg, P., Hansen, H. G., Lee, G. M., Andersen, M. R. & Kildegaard, H. F., 2019, In : ACS Synthetic Biology. 8, 4, p. 758-774 17 p.

Research output: Contribution to journalJournal articleResearchpeer-review

2018
114 Downloads (Pure)

An Online Compendium of CHO RNA-Seq Data Allows Identification of CHO Cell Line-specific Transcriptomic Signatures

Singh, A., Kildegaard, H. F. & Andersen, M. R., 2018, In : Biotechnology Journal. 13, 10, 11 p., 1800070.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
263 Downloads (Pure)

Baicalein reduces oxidative stress in CHO cell cultures and improves recombinant antibody productivity

Kwang Ha, T., Hansen, A. H., Kol, S., Kildegaard, H. F. & Min Lee, G., 2018, In : Biotechnology Journal. 13, 3, 10 p., 1700425.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
192 Downloads (Pure)

Comprehensive characterization of glutamine synthetase-mediated selection for the establishment of recombinant CHO cells producing monoclonal antibodies

Noh, S. M., Shin, S. & Min Lee, G., 2018, In : Scientific Reports. 8, 11 p., 5361.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
80 Downloads (Pure)

CRISPR/Cas9-multiplexed editing of Chinese hamster ovary B4Gal-T1, 2, 3 and 4 Tailors N-Glycan Profiles of Therapeutics and Secreted Host Cell Proteins

Amann, T., Hansen, A. H., Kol, S., Min Lee, G., Andersen, M. R. & Kildegaard, H. F., 2018, In : Biotechnology Journal. 13, 10, 9 p., 1800111 .

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
223 Downloads (Pure)

Enhanced genome editing tools for multi-gene deletion knock-out approaches using paired CRISPR sgRNAs in CHO cells

Schmieder, V., Bydlinski, N., Strasser, R., Baumann, M., Kildegaard, H. F., Jadhav, V. & Borth, N., 2018, In : Biotechnology Journal. 13, 3, 10 p., 1700211.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
884 Downloads (Pure)

Impact of CHO Metabolism on Cell Growth and Protein Production: An Overview of Toxic and Inhibiting Metabolites and Nutrients

Pereira, S., Kildegaard, H. F. & Andersen, M. R., 2018, In : Biotechnology Journal. 13, 3, 13 p., 1700499 .

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File

Minimizing Clonal Variation during Mammalian Cell Line Engineering for Improved Systems Biology Data Generation

Grav, L. M., Sergeeva, D., Lee, J. S., Marín de Mas, I., Lewis, N. E., Andersen, M. R., Nielsen, L. K., Min Lee, G. & Kildegaard, H. F., 2018, In : A C S Synthetic Biology. 7, 9, p. 2148-2159

Research output: Contribution to journalJournal articleResearchpeer-review

101 Downloads (Pure)

Modular 5′-UTR hexamers for context-independent tuning of protein expression in eukaryotes

Petersen, S. D., Zhang, J., Lee, J. S., Jakočiūnas, T., Grav, L. M., Kildegaard, H. F., Keasling, J. D. & Jensen, M. K., 2018, In : Nucleic acids research. 46, 21, 11 p., e127.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
3 Downloads (Pure)

Revealing key determinants of clonal variation in transgene expression in recombinant CHO cells using targeted genome editing

Lee, J. S., Park, J. H., Kwang Ha, T., Samoudi, M., Lewis, N. E., Palsson, B. O., Kildegaard, H. F. & Min Lee, G., 2018, In : A C S Synthetic Biology. 7, 12, p. 2867-2878

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
203 Downloads (Pure)
Open Access
File
2017
323 Downloads (Pure)

Cell Factory Engineering

Davy, A. M., Kildegaard, H. F. & Andersen, M. R., 2017, In : Cell Systems. 4, 3, p. 262-275

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
224 Downloads (Pure)

Developing a CRISPR/Cas9 screening platform for Chinese hamster ovary cells

Karottki, K. J. L. C., Li, S., Pedersen, L. E., Hefzi, H., Spahn, P., Thomas, A., Lee, J. S., Lee, G. M., Lewis, N. E. & Kildegaard, H. F., 2017, In : FEBS Journal. 284, Suppl. 1, p. 172-172 1 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
1970 Downloads (Pure)

Improving the secretory capacity of Chinese hamster ovary cells by ectopic expression of effector genes: Lessons learned and future directions

Hansen, H. G., Pristovsek, N., Kildegaard, H. F. & Min Lee, G., 2017, In : Biotechnology Advances. 35, 1, p. 64–76 35 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
241 Downloads (Pure)

Network reconstruction of the mouse secretory pathway applied on CHO cell transcriptome data

Lund, A. M., Kaas, C. S., Brandl, J., Pedersen, L. E., Kildegaard, H. F., Kristensen, C. & Andersen, M. R., 2017, In : B M C Systems Biology. 11, 1, 17 p., 37.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
370 Downloads (Pure)

Ribosome profiling-guided depletion of an mRNA increases cell growth rate and protein secretion

Beuchert Kallehauge, T., Li, S., Pedersen, L. E., Kwang Ha, T., Ley, D., Andersen, M. R., Kildegaard, H. F., Min Lee, G. & Lewis, N. E., 2017, In : Scientific Reports. 7, 12 p., 40388.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
2016

Accelerated Homology-Directed Targeted Integration of Transgenes in Chinese Hamster Ovary Cells Via CRISPR/Cas9 and Fluorescent Enrichment

Lee, J. S., Grav, L. M., Pedersen, L. E., Min Lee, G. & Kildegaard, H. F., 2016, In : Biotechnology and Bioengineering (Print). 113, 11, p. 2518-2523 6 p.

Research output: Contribution to journalJournal articleResearchpeer-review

A Consensus Genome-scale Reconstruction of Chinese Hamster Ovary Cell Metabolism

Hefzi, H., Ang, K. S., Hanscho, M., Bordbar, A., Ruckerbauer, D., Lakshmanan, M., Orellana, C. A., Baycin-Hizal, D., Huang, Y., Ley, D., Martinez, V. S., Kyriakopoulos, S., Jiménez, N. E., Zielinski, D. C., Quek, L-E., Wulff, T., Arnsdorf, J., Li, S., Lee, J. S., Paglia, G. & 20 others, Loira, N., Spahn, P. N., Pedersen, L. E., Gutierrez, J. M., King, Z. A., Lund, A. M., Nagarajan, H., Thomas, A., Abdel-Haleem, A. M., Zanghellini, J., Kildegaard, H. F., Voldborg, B. G., Gerdtzen, Z. P., Betenbaugh, M. J., Palsson, B., Andersen, M. R., Nielsen, L. K., Borth, N., Lee, D-Y. & Lewis, N. E., 2016, In : Cell Systems. 3, 5, p. 434-443

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
1 Downloads (Pure)

A Markov chain model for N-linked protein glycosylation – towards a low-parameter tool for model-driven glycoengineering

Spahn, P. N., Hansen, A. H., Hansen, H. G., Arnsdorf, J., Kildegaard, H. F. & Lewis, N., 2016, In : Metabolic Engineering. 33, p. 52-66 15 p.

Research output: Contribution to journalJournal articleResearchpeer-review

555 Downloads (Pure)

Case study on human α1-antitrypsin: Recombinant protein titers obtained by commercial ELISA kits are inaccurate

Hansen, H. G., Kildegaard, H. F., Min Lee, G. & Stefan Kol, S., 2016, In : Biotechnology Journal. 11, 12, p. 1648–1656 9 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File

Chemical Inhibition of Autophagy: Examining its Potential to Increase the Specific Productivity of Recombinant CHO Cell Lines

Baek, E., Lin Kim, C., Gyeom Kim, M., Lee, J. S. & Min Lee, G., 2016, In : Biotechnology and Bioengineering. 113, 9, p. 1953-1961

Research output: Contribution to journalJournal articleResearchpeer-review

Endoplasmic reticulum-directed recombinant mRNA displays subcellular localization equal to endogenous mRNA during transient expression in CHO cells

Beuchert Kallehauge, T., Kol, S., Andersen, M. R., Kroun Damgaard, C., Min Lee, G. & Kildegaard, H. F., 2016, In : Biotechnology Journal. 11, 10, p. 1362-1367 8 p.

Research output: Contribution to journalJournal articleResearchpeer-review

2015

CRISPR/Cas9-mediated genome engineering of CHO cell factories: application and perspectives

Lee, J. S., Grav, L. M., Lewis, N. E. & Kildegaard, H. F., 2015, In : Biotechnology Journal. 10, 7, p. 979-994 16 p.

Research output: Contribution to journalJournal articleResearchpeer-review

216 Downloads (Pure)

Development of a VHH-Based Erythropoietin Quantification Assay

Kol, S., Beuchert Kallehauge, T., Adema, S. & Hermans, P., 2015, In : Molecular Biotechnology. 57, 8, p. 692-700 9 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File

Glycoengineering of Chinese hamster ovary cells for enhanced erythropoietin N-glycan branching and sialylation

Yin, B., Gao, Y., Chung, C., Yang, S., Blake, E., C. Stuczynski, M., Tang, J., Kildegaard, H. F., Andersen, M. R., Zhang, H. & Betenbaugh, M. J., 2015, In : Biotechnology and Bioengineering (Print). 112, 11, p. 2343-2350 8 p.

Research output: Contribution to journalJournal articleResearchpeer-review

422 Downloads (Pure)

Multi-omic profiling of EPO-producing Chinese hamster ovary cell panel reveals metabolic adaptation to heterologous protein production

Ley, D., Kazemi Seresht, A., Engmark, M., Magdenoska, O., Nielsen, K. F., Kildegaard, H. F. & Andersen, M. R., 2015, In : Biotechnology and Bioengineering. 112, 11, p. 2373-2387 15 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File

One-step generation of triple knockout CHO cell lines using CRISPR/Cas9 and fluorescent enrichment.

Grav, L. M., Lee, J. S., Thomsen, S. G., Beuchert Kallehauge, T., Hansen, A. H., Kol, S., Min Lee, G., Pedersen, L. E. & Kildegaard, H. F., 2015, In : Biotechnology Journal. 10, 9, p. 1446-1456 11 p.

Research output: Contribution to journalJournal articleResearchpeer-review

318 Downloads (Pure)

Site-specific integration in CHO cells mediated by CRISPR/Cas9 and homology-directed DNA repair pathway

Lee, J. S., Beuchert Kallehauge, T., Pedersen, L. E. & Kildegaard, H. F., 2015, In : Scientific Reports. 5, 11 p., 8572.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
259 Downloads (Pure)

Versatile microscale screening platform for improving recombinant protein productivity in Chinese hamster ovary cells

Hansen, H. G., Nilsson, C. N., Lund, A. M., Kol, S., Grav, L. M., Lundqvist, M., Rockberg, J., Min Lee, G., Andersen, M. R. & Kildegaard, H. F., 2015, In : Scientific Reports. 5, 12 p., 18016 .

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
2014
1 Downloads (Pure)

Accelerating Genome Editing in CHO Cells Using CRISPR Cas9 and CRISPy, a Web-Based Target Finding Tool

Ronda, C., Pedersen, L. E., Hansen, H. G., Beuchert Kallehauge, T., Betenbaugh, M., Nielsen, A. T. & Kildegaard, H. F., 2014, In : Biotechnology and Bioengineering (Print). 111, 8, p. 1604-1616

Research output: Contribution to journalJournal articleResearchpeer-review

473 Downloads (Pure)

A Versatile System for USER Cloning-Based Assembly of Expression Vectors for Mammalian Cell Engineering

Lund, A. M., Kildegaard, H. F., Petersen, M. B. K., Rank, J., Hansen, B. G., Andersen, M. R. & Mortensen, U. H., 2014, In : P L o S One. 9, 5, 10 p., e96693.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File

Toward genome-scale models of the Chinese hamster ovary cells: incentives, status and perspectives

Kaas, C. S., Fan, Y., Weilguny, D., Kristensen, C., Kildegaard, H. F. & Andersen, M. R., 2014, In : Pharmaceutical Bioprocessing. 2, 5, p. 437-448

Research output: Contribution to journalJournal articleResearchpeer-review

2013
721 Downloads (Pure)

Genomic landscapes of Chinese hamster ovary cell lines as revealed by the Cricetulus griseus draft genome.

Lewis, N. E., Liu, X., Li, Y., Nagarajan, H., Yerganian, G., O'Brien, E., Bordbar, A., Roth, A. M., Rosenbloom, J., Bian, C., Xie, M., Chen, W., Li, N., Baycin-Hizal, D., Latif, H., Förster, J., Betenbaugh, M., Famili, I., Xu, X., Wang, J. & 1 others, Palsson, B. O., 2013, In : Nature Biotechnology. 31, p. 759-765

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
1 Downloads (Pure)

The emerging CHO systems biology era: harnessing the ‘omics revolution for biotechnology

Kildegaard, H. F., Baycin-Hizal, D., Lewis, N. & Betenbaugh, M., 2013, In : Current Opinion in Biotechnology. 24, 6, p. 1102-1107

Research output: Contribution to journalJournal articleResearchpeer-review