Research Output

2020

Awakening dormant glycosyltransferases in CHO cells with CRISPRa

Julie la Cour Karottki, K., Hefzi, H., Xiong, K., Shamie, I., Hansen, A. H., Li, S., Pedersen, L. E., Li, S., Lee, J. S., Min Lee, G., Kildegaard, H. F. & Lewis, N. E., 2020, In : Biotechnology and Bioengineering. 117, 2, p. 593-598

Research output: Contribution to journalJournal articleResearchpeer-review

Knockout of sialidase and pro-apoptotic genes in Chinese hamster ovary cells enables the production of recombinant human erythropoietin in fed-batch cultures

Ha, T. K., Hansen, A. H., Kildegaard, H. F. & Lee, G. M., 2020, In : Metabolic Engineering. 57, p. 182-192

Research output: Contribution to journalJournal articleResearchpeer-review

2019

BCAT1 and BCAT2 disruption in CHO cells has cell line-dependent effects

Pereira, S., Ley, D., Schubert, M., Grav, L. M., Kildegaard, H. F. & Andersen, M. R., 2019, In : Journal of Biotechnology. 306, p. 24-31 8 p.

Research output: Contribution to journalJournal articleResearchpeer-review

BiP Inducer X: An ER Stress Inhibitor for Enhancing Recombinant Antibody Production in CHO Cell Culture

Kwang Ha, T., Hansen, A. H., Kildegaard, H. F. & Min Lee, G., 2019, In : Biotechnology Journal. e1900130.

Research output: Contribution to journalJournal articleResearchpeer-review

CRISPR/Cas9 as a Genome Editing Tool for Targeted Gene Integration in CHO Cells

Sergeeva, D., Manuel Camacho Zaragoza, J., Lee, J. S. & Kildegaard, H. F., 2019, CRISPR Gene Editing. Vol. 1961. p. 213-232 (Methods in Molecular Biology, Vol. 1961).

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

CRISPR Toolbox for Mammalian Cell Engineering

Sergeeva, D., Julie la Cour Karottki, K., Lee, J. S. & Kildegaard, H. F., 2019, Cell Culture Engineering: Recombinant Protein Production.

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

Expanding the CRISPR Toolbox for Chinese Hamster Ovary Cell Line Engineering

Julie la Cour Karottki, K., 2019, Technical University of Denmark. 155 p.

Research output: Book/ReportPh.D. thesis

Open Access
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1 Downloads (Pure)

Genetic engineering approaches to improve post-translational modification of biopharmaceuticals in different production platforms

Amann, T., Schmieder, V., Kildegaard, H. F., Borth, N. & Andersen, M. R., 2019, In : Biotechnology and Bioengineering. 116, 10, p. 2778-2796

Research output: Contribution to journalJournal articleResearchpeer-review

Genome-wide systematic identification of methyltransferase recognition and modification patterns

Jensen, T. Ø., Tellgren-Roth, C., Redl, S. M. A., Maury, J., Baallal Jacobsen, S. A., Pedersen, L. E. & Nielsen, A. T., 2019, In : Nature Communications. 10, 1, 3311.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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87 Downloads (Pure)

Glyco-engineered CHO cell lines producing alpha-1-antitrypsin and C1 esterase inhibitor with fully humanized N-glycosylation profiles

Amann, T., Hansen, A. H., Kol, S., Hansen, H. G., Arnsdorf, J., Nallapareddy, S., Voldborg, B., Min Lee, G., Andersen, M. R. & Kildegaard, H. F., 2019, In : Metabolic Engineering. 52, p. 143-152 10 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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47 Downloads (Pure)

Glycosylation of proteins

Voldborg, B., Kol, S., Hansen, A. & Kildegaard, H., 6 Jun 2019, IPC No. C12P 21/ 02 A I, Patent No. WO2019105770, 28 Nov 2017, Priority date 11 Jul 2018, Priority No. EP20180182948

Research output: Patent

Open Access
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25 Downloads (Pure)

Improving recombinant bone morphogenetic protein-4 (BMP-4) production by autoregulatory feedback loop removal using BMP receptor-knockout CHO cell lines

Kim, C. L. & Min Lee, G., 2019, In : Metabolic Engineering. 52, p. 57-67

Research output: Contribution to journalJournal articleResearchpeer-review

Mitigating Clonal Variation in Recombinant Mammalian Cell Lines

Lee, J. S., Kildegaard, H. F., Lewis, N. E. & Min Lee, G., 2019, In : Trends in Biotechnology. 37, 9, p. 931-942 12 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Reduced apoptosis in Chinese hamster ovary cells via optimized CRISPR interference

Xiong, K., Marquart, K. F., Julie la Cour Karottki, K., Li, S., Shamie, I., Lee, J. S., Gerling, S., Yeo, N. C., Chavez, A., Min Lee, G., Lewis, N. E. & Kildegaard, H. F., 2019, In : Biotechnology and Bioengineering. 116, 7, p. 1813-1819 7 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Reprogramming AA catabolism in CHO cells with CRISPR/Cas9 genome editing improves cell growth and reduces byproduct secretion

Ley, D., Pereira, S., Pedersen, L. E., Arnsdorf, J., Hefzi, H., Davy, A. M., Kwang Ha, T., Wulff, T., Kildegaard, H. F. & Andersen, M. R., 2019, In : Metabolic Engineering. 56, p. 120-129 10 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Systematic Evaluation of Site-Specific Recombinant Gene Expression for Programmable Mammalian Cell Engineering

Pristovšek, N., Nallapareddy, S., Grav, L. M., Hefzi, H., Lewis, N. E., Rugbjerg, P., Hansen, H. G., Lee, G. M., Andersen, M. R. & Kildegaard, H. F., 2019, In : ACS Synthetic Biology. 8, 4, p. 758-774 17 p.

Research output: Contribution to journalJournal articleResearchpeer-review

2018
Open Access
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722 Downloads (Pure)

An Online Compendium of CHO RNA-Seq Data Allows Identification of CHO Cell Line-specific Transcriptomic Signatures

Singh, A., Kildegaard, H. F. & Andersen, M. R., 2018, In : Biotechnology Journal. 13, 10, 11 p., 1800070.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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114 Downloads (Pure)

Baicalein reduces oxidative stress in CHO cell cultures and improves recombinant antibody productivity

Kwang Ha, T., Hansen, A. H., Kol, S., Kildegaard, H. F. & Min Lee, G., 2018, In : Biotechnology Journal. 13, 3, 10 p., 1700425.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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263 Downloads (Pure)
Open Access
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45 Downloads (Pure)

Comprehensive characterization of glutamine synthetase-mediated selection for the establishment of recombinant CHO cells producing monoclonal antibodies

Noh, S. M., Shin, S. & Min Lee, G., 2018, In : Scientific Reports. 8, 11 p., 5361.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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192 Downloads (Pure)

CRISPR/Cas9-multiplexed editing of Chinese hamster ovary B4Gal-T1, 2, 3 and 4 Tailors N-Glycan Profiles of Therapeutics and Secreted Host Cell Proteins

Amann, T., Hansen, A. H., Kol, S., Min Lee, G., Andersen, M. R. & Kildegaard, H. F., 2018, In : Biotechnology Journal. 13, 10, 9 p., 1800111 .

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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80 Downloads (Pure)

Design of Optimal CHO Protein N-glycosylation Profiles

Amann, T., 2018, Kgs. Lyngby: Technical University of Denmark. 142 p.

Research output: Book/ReportPh.D. thesisResearch

Open Access
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382 Downloads (Pure)
Open Access
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455 Downloads (Pure)

Enhanced genome editing tools for multi-gene deletion knock-out approaches using paired CRISPR sgRNAs in CHO cells

Schmieder, V., Bydlinski, N., Strasser, R., Baumann, M., Kildegaard, H. F., Jadhav, V. & Borth, N., 2018, In : Biotechnology Journal. 13, 3, 10 p., 1700211.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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223 Downloads (Pure)

Impact of CHO Metabolism on Cell Growth and Protein Production: An Overview of Toxic and Inhibiting Metabolites and Nutrients

Pereira, S., Kildegaard, H. F. & Andersen, M. R., 2018, In : Biotechnology Journal. 13, 3, 13 p., 1700499 .

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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884 Downloads (Pure)

Method for reducing ammonium and lactate production in cho cells

Ley, D., Andersen, M. R. & Kildegaard, H. F., 2018, IPC No. C12N15/52; C12P21/02, Patent No. WO2018033542, 22 Feb 2018, Priority date 15 Aug 2016, Priority No. EP20160184179

Research output: Patent

Open Access
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160 Downloads (Pure)

Minimizing Clonal Variation during Mammalian Cell Line Engineering for Improved Systems Biology Data Generation

Grav, L. M., Sergeeva, D., Lee, J. S., Marín de Mas, I., Lewis, N. E., Andersen, M. R., Nielsen, L. K., Min Lee, G. & Kildegaard, H. F., 2018, In : A C S Synthetic Biology. 7, 9, p. 2148-2159

Research output: Contribution to journalJournal articleResearchpeer-review

Modular 5′-UTR hexamers for context-independent tuning of protein expression in eukaryotes

Petersen, S. D., Zhang, J., Lee, J. S., Jakočiūnas, T., Grav, L. M., Kildegaard, H. F., Keasling, J. D. & Jensen, M. K., 2018, In : Nucleic acids research. 46, 21, 11 p., e127.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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101 Downloads (Pure)

Revealing key determinants of clonal variation in transgene expression in recombinant CHO cells using targeted genome editing

Lee, J. S., Park, J. H., Kwang Ha, T., Samoudi, M., Lewis, N. E., Palsson, B. O., Kildegaard, H. F. & Min Lee, G., 2018, In : A C S Synthetic Biology. 7, 12, p. 2867-2878

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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3 Downloads (Pure)

Targeting miRNAs with CRISPR/Cas9 to Improve Recombinant Protein Production of CHO Cells

Kellner, K., Solanki, A., Amann, T., Lao, N. & Barron, N., 2018, Recombinant Protein Expression in Mammalian Cells . Walker, J. M. (ed.). Humana Press, Vol. 1850. p. 221-235 (Methods in Molecular Biology).

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

Using titer and titer normalized to confluence are complementary strategies for obtaining Chinese hamster ovary cell lines with high volumetric productivity of etanercept

Pristovšek, N., Hansen, H. G., Sergeeva, D., Borth, N., Min Lee, G., Andersen, M. R. & Kildegaard, H. F., 2018, In : Biotechnology Journal. 13, 3, 10 p., 1700216.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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203 Downloads (Pure)
2017

Application of CRISPR/Cas9 Genome Editing to Improve Recombinant Protein Production in CHO Cells

Grav, L. M., Julie la Cour Karottki, K., Lee, J. S. & Kildegaard, H. F., 2017, Heterologous Protein Production in CHO cells. Springer, p. 101-118 (Methods in Molecular Biology, Vol. 1603).

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

1 Downloads (Pure)

Cell Factory Engineering

Davy, A. M., Kildegaard, H. F. & Andersen, M. R., 2017, In : Cell Systems. 4, 3, p. 262-275

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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323 Downloads (Pure)

CHO glyco-engineering using CRISPR/Cas9 multiplexing for protein production with homogeneous N-glycan profiles

Amann, T., Hansen, A. H., Pristovsek, N., Singh, A., Min Lee, G., Andersen, M. R. & Kildegaard, H. F., 2017. 1 p.

Research output: Contribution to conferencePosterResearchpeer-review

Open Access
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227 Downloads (Pure)

Developing a CRISPR/Cas9 screening platform for Chinese hamster ovary cells

Karottki, K. J. L. C., Li, S., Pedersen, L. E., Hefzi, H., Spahn, P., Thomas, A., Lee, J. S., Lee, G. M., Lewis, N. E. & Kildegaard, H. F., 2017, In : FEBS Journal. 284, Suppl. 1, p. 172-172 1 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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224 Downloads (Pure)

Engineer medium and feed for modulating N-glycosylation of recombinant protein production in CHO cell culture

Fan, Y., Kildegaard, H. F. & Andersen, M. R., 2017, Heterologous Protein Production in CHO cells. Springer, p. 209-226 (Methods in Molecular Biology, Vol. 1603).

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

Humanizing recombinant glycoproteins from Chinese hamster ovary cells

Hansen, A. H., Amann, T., Kol, S. & Kildegaard, H. F., 2017.

Research output: Contribution to conferenceConference abstract for conferenceResearchpeer-review

Open Access
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85 Downloads (Pure)

Improving the secretory capacity of Chinese hamster ovary cells by ectopic expression of effector genes: Lessons learned and future directions

Hansen, H. G., Pristovsek, N., Kildegaard, H. F. & Min Lee, G., 2017, In : Biotechnology Advances. 35, 1, p. 64–76 35 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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1970 Downloads (Pure)

Network reconstruction of the mouse secretory pathway applied on CHO cell transcriptome data

Lund, A. M., Kaas, C. S., Brandl, J., Pedersen, L. E., Kildegaard, H. F., Kristensen, C. & Andersen, M. R., 2017, In : B M C Systems Biology. 11, 1, 17 p., 37.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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241 Downloads (Pure)

Reprogramming amino acid catabolism in CHO cells with CRISPR-Cas9 genome editing improves cell growth and reduces by-product secretion

Ley, D., Pereira, S., Pedersen, L. E., Arnsdorf, J., Hefzi, H., Lund, A. M., Kwang Ha, T., Wulff, T., Kildegaard, H. F. & Andersen, M. R., 2017. 1 p.

Research output: Contribution to conferencePosterResearchpeer-review

File
443 Downloads (Pure)

Ribosome profiling-guided depletion of an mRNA increases cell growth rate and protein secretion

Beuchert Kallehauge, T., Li, S., Pedersen, L. E., Kwang Ha, T., Ley, D., Andersen, M. R., Kildegaard, H. F., Min Lee, G. & Lewis, N. E., 2017, In : Scientific Reports. 7, 12 p., 40388.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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370 Downloads (Pure)
2016

Accelerated Homology-Directed Targeted Integration of Transgenes in Chinese Hamster Ovary Cells Via CRISPR/Cas9 and Fluorescent Enrichment

Lee, J. S., Grav, L. M., Pedersen, L. E., Min Lee, G. & Kildegaard, H. F., 2016, In : Biotechnology and Bioengineering (Print). 113, 11, p. 2518-2523 6 p.

Research output: Contribution to journalJournal articleResearchpeer-review

A Consensus Genome-scale Reconstruction of Chinese Hamster Ovary Cell Metabolism

Hefzi, H., Ang, K. S., Hanscho, M., Bordbar, A., Ruckerbauer, D., Lakshmanan, M., Orellana, C. A., Baycin-Hizal, D., Huang, Y., Ley, D., Martinez, V. S., Kyriakopoulos, S., Jiménez, N. E., Zielinski, D. C., Quek, L-E., Wulff, T., Arnsdorf, J., Li, S., Lee, J. S., Paglia, G. & 20 others, Loira, N., Spahn, P. N., Pedersen, L. E., Gutierrez, J. M., King, Z. A., Lund, A. M., Nagarajan, H., Thomas, A., Abdel-Haleem, A. M., Zanghellini, J., Kildegaard, H. F., Voldborg, B. G., Gerdtzen, Z. P., Betenbaugh, M. J., Palsson, B., Andersen, M. R., Nielsen, L. K., Borth, N., Lee, D-Y. & Lewis, N. E., 2016, In : Cell Systems. 3, 5, p. 434-443

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access

A Markov chain model for N-linked protein glycosylation – towards a low-parameter tool for model-driven glycoengineering

Spahn, P. N., Hansen, A. H., Hansen, H. G., Arnsdorf, J., Kildegaard, H. F. & Lewis, N., 2016, In : Metabolic Engineering. 33, p. 52-66 15 p.

Research output: Contribution to journalJournal articleResearchpeer-review

1 Downloads (Pure)

Case study on human α1-antitrypsin: Recombinant protein titers obtained by commercial ELISA kits are inaccurate

Hansen, H. G., Kildegaard, H. F., Min Lee, G. & Stefan Kol, S., 2016, In : Biotechnology Journal. 11, 12, p. 1648–1656 9 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
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555 Downloads (Pure)

Chemical Inhibition of Autophagy: Examining its Potential to Increase the Specific Productivity of Recombinant CHO Cell Lines

Baek, E., Lin Kim, C., Gyeom Kim, M., Lee, J. S. & Min Lee, G., 2016, In : Biotechnology and Bioengineering. 113, 9, p. 1953-1961

Research output: Contribution to journalJournal articleResearchpeer-review

CHO On A Detox: Removing By-Product Formation Through Cell Engineering

Pereira, S., Kildegaard, H. F. & Andersen, M. R., 2016. 1 p.

Research output: Contribution to conferenceConference abstract for conferenceResearchpeer-review

Open Access
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Consortia based production of biochemicals

Ingemann Jensen, S., Sukumara, S., Özdemir, E., Schneider, K., Calero Valdayo, P. M., Li, S., Ronda, C. & Nielsen, A. T., 2016. 1 p.

Research output: Contribution to conferenceConference abstract for conferenceResearchpeer-review

Open Access