Using microfluids to study programmed cell death: a new approach

Christina Mark (Speaker)

    Activity: Talks and presentationsConference presentations

    Description

    Poster elevator talk, 7 min

    ABSTRACT: This project focuses on applying microfluidic tissue culture for electrochemical or optical measurements during programmed cell death (PCD) in barley aleurone layer to increase understanding of the underlying mechanisms of PCD in plants. Microfluidic tissue culture enables in vitro experiments to approach in vivo conditions. Microfluidics also allow implementation of a wide range of electrochemical or optical assays for online, real-time, parallel analysis of important parameters such as redox activity, O2 and H2O2 concentration, extracellular pH, cell viability and enzyme activity [1,2]. Currently, we are optimising an intracellular whole-cell redox activity assay [3] that detects changes in redox activity in barley aleurone layer during PCD. The assay uses a double mediator-system to electrochemically measure redox activity via changes in the NADP:NADPH ratio. Initial experiments assay show that the redox activity changes depending on phytohormone activation or inactivation of aleurone layer metabolism and subsequent PCD. This is similar to H2O2 concentration changes observed recently by Ishibashi et al [4]. We have also successfully detected PCD induced by phytohormones in barley aleurone layer using a double-fluorescent probe-system also used by Fath et al [5]. Future challenges include integrating both these systems into a microfluidic device for plant tissue culture. References [1] Xu et al., Talanta, 2009, 80, 8-18, 2009 [2] Sassa et al, Electrophoresis, 2008, 29, 1787-1800, 2008 [3] Heiskanen et al., Anal Biochem, 2009, 384, 11-19, 2009 [4] Ishibashi et al., Plant Physiol, 158, 1705-1714, 2012 [5] Fath et al., Plant Physiol, 126, 156-166, 2001
    Period11 Aug 201315 Aug 2013
    Event title25th Congress of the Scandinavian Plant Physiology Society
    Event typeConference
    Conference number25
    LocationHelsingør, Denmark