DescriptionThe response to pathogens and damage in vertebrates involves a series of organized and highly evolved molecular mechanisms leading to pathogen specific immune reactions and tissue regeneration as the wound healing process. Pathogen and damage-associated molecular patterns (PAMPs and DAMPs) rely on pattern recognition receptors (PRRs) and down stream signalling pathways, promoting immune reactions. During the last years, the ability of β-glucans to modulate immunity through activation of innate cellular components has been observed. However, toxicological effects associated with the systemic administration and dose-related immune-suppression has also been described. The superior aim of this study is to understand the effect of β-glucan induced modulation in carp in relation to tissue regeneration, mucosal immunity and host-pathogen interactions. A comparison in modulation between immune cells and tissue related cells will be evaluated. Parameters as respiratory burst activity and expression profiles of immune related genes will be measured in cell cultures and in in vivo experiments. The methodology of the project involves the usage of real-time quantitative PCR to quantify expression of genes of interest (IL-1β, IL-10, TNF-α, TGF-β, COL-1A, MMP, FGFs and IGFs). This study will provide further understanding on the effect of β-glucan as a modulator which could improve fish welfare and health as well as having an economic potential production for the aquaculture industry.
|3 May 2009 → 5 May 2009
|Poster presentation: ß-glucan as immune modulator in Common Carp (Cyprinus carpio)