Description
We introduce a compact attachment for microscope objectives that allows for the conversion of conventional fluorescence microscopes into Airy light-sheet microscopes. The attachment includes a one-dimensional Airy beam generator, which comprises a gradient-index collimator and a 3D nano-printed cubic phase-plate, realized through two-photon polymerization 3D nano-printing and a two-step writing process that guarantees an optical-quality surface for the phase plate. The micro-optical unit is affixed to a mechanical holder equipped with micro-stages, thereby facilitating the unit's integration into commercial microscopes. The implementation and imaging performance of this system and its fundamental imaging characteristics are discussed, with findings based on diverse samples.Period | 7 Apr 2024 → 11 Jul 2024 |
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Event title | SPIE Photonics Europe 2024 |
Event type | Conference |
Location | Strasbourg, FranceShow on map |
Degree of Recognition | International |
Keywords
- lightsheet
- microscopy
- two-photon
- multiphoton
- endoscopy
- Airy
- miniaturization
- fiber
- nano-printing
- phase plate
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Fast Volumetric Two-Photon in vivo Imaging
Project: PhD