DescriptionAttenuation of optical fields owing to scattering and absorption limits the penetration depth into tissue. Whilst aberration correction may be used this is difficult to implement over a large field of view in heterogeneous tissue. Recently, the novel approach of attenuation-compensation of propagation-invariant light fields has shown increase in depth penetration for light-sheet microscopy. Here we show this powerful approach may be implemented in a facile manner utilizing a graded neutral density filter circumventing the need for expensive beam shaping apparatus. A ‘gold standard’ system utilizing a spatial light modulator for beam shaping is used to benchmark our low-cost implementation.
|Period||4 Feb 2020|
|Event title||SPIE Photonics West 2020: Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVII|
|Location||San Francisco, United States, California|
|Degree of Recognition||International|
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