Imaging the airy way: Advanced beam shaping for light-sheet microscopy at depth

Veettikazhy, M. (Speaker)

Activity: Talks and presentationsConference presentations

Description

Attenuation of optical fields owing to scattering and absorption limits the penetration depth into tissue. Whilst aberration correction may be used this is difficult to implement over a large field of view in heterogeneous tissue. Recently, the novel approach of attenuation-compensation of propagation-invariant light fields has shown increase in depth penetration for light-sheet microscopy. Here we show this powerful approach may be implemented in a facile manner utilizing a graded neutral density filter circumventing the need for expensive beam shaping apparatus. A ‘gold standard’ system utilizing a spatial light modulator for beam shaping is used to benchmark our low-cost implementation.
Period4 Feb 2020
Event titleSPIE Photonics West 2020: Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVII
Event typeConference
Conference number11245-17
LocationSan Francisco, United States, California
Degree of RecognitionInternational