Description
AbstractWith the increasing use of nanotechnology in food and consumer products, there is a need for reliable detection and characterization methods for nanoparticles (NPs) in complex matrices. Asymmetric flow field-flow fractionation (AF4) coupled to inductively coupled plasma mass spectrometry (ICP–MS) is a highly promising method for this purpose. However, the number of publications regarding sample preparation of NPs in organic matrices including food stuffs, tissues and cells for AF4–ICP–MS is still limited. Silver nanoparticles (AgNPs) are presently one of the most frequently used nanomaterials in consumer products related to food, such as food storage containers and dietary supplements. In the presented work AgNPs were incorporated into chicken meat to illustrate a possible scenario where AgNPs may migrate from an antibacterial food contact material into meat. A method of analysis was developed based on enzymatic digestion followed by AF4-ICP–MS fractionation and detection. The method was validated in terms of precision, reproducibility, linearity and limit of detection / quantification. In addition, single particle ICP–MS was applied for determination of the number-based particle size distribution of AgNPs in collected fractions. The talk will describe which methodological steps were necessary, and highlight the challenges that had to be addressed, in order to develop an appropriate sample preparation method for AgNPs in meat.
Keywords: Silver nanoparticles, food, field flow fractionation, single particle ICP–MS, enzymatic digestion
Acknowledgement: The work leading to these results has received funding from the European Union Seventh Framework Programme (FP7/2007–2013) under grant agreement n° 245162.
| Period | 6 Nov 2013 |
|---|---|
| Event title | 6th International Symposium on Recent Advances in Food Analysis |
| Event type | Conference |
| Conference number | 6 |
| Location | Prague, Czech RepublicShow on map |