Description

CRISPR-Cas9 is a rapidly expanding toolbox used in several aspects of biological research, and our group have developed several CRISPR/Cas9 tools for yeast.1–4
In our current research, we have designed a study in yeast that aims to perturb flux through candidate metabolic pathways by expression tuning using nuclease- deficient Cas9 (dCas9) fused with regulatory elements of transcription together with guide RNAs targeting promoters of interest. Through a robust promoter- reporter assay we investigate the regulatory power of dCas9-associated effectors on relevant pathway promoters in vivo. Our study targets in-depth knowledge about static and dynamic dCas9-mediated transcriptional regulation in yeast, and how this can be used to tune expression of key metabolic pathway genes. In our research, we have a particular interest towards optimization of bio-based production of chemicals in yeast cell factories. Bio-based production of chemicals in yeast appeals, because it typically reduces the environmental footprint compared to chemical synthesis derived from fossil fuels.5 Hence, our research is expected to lead to the identification of yeast cell factories with balanced regulation of multi-step biosynthetic pathway genes and increased production of candidate chemicals.


Oral presentation (15 min)

Period	8 Jun 2016
Event title	FASEB 2016: Genome Engineering: Cutting-Edge Research and Applications
Event type	Conference
Location	Lisbon, PortugalShow on map