Adaptive Laboratory Evolution of Saccharomyces cerevisiae Diploid Strains for Mannitol Utilization as a Carbon Source

Javier Porcayo Loza (Speaker), Mortensen, U. H. (Other)

Activity: Talks and presentationsConference presentations

Description

Crude oil reserves are becoming increasingly scarce, and biorefinery systems that integrate biomass conversion processes and equipment to produce fuels, power, and chemicals from annually renewable resources are a promising technology to move away from a petroleum- based society to a biomass-based society. One interesting biomass that has not been extensively utilized is marine biomass such as brown macroalgae (kelp). The composition of brown macroalgae includes up to 55% dry weight of the carbohydrates laminarin, mannitol and alginate, and it does not contain lignin. Hence, macroalgae are a very promising feedstock for microbial conversion of all carbohydrates into biofuels and valuable chemicals. Despite the presence of this native catabolic pathway, many yeast strains cannot catabolize mannitol or require adaptation to do so.
In this study a screening of thirty six strains, isolated from different sources, was performed. The strains were grown on complex and minimal media with mannitol as a main carbon source. Fifteen strains showed growth on complex media-mannitol (CM-mannitol) and just three diploid strains were capable to growth on minimal media-mannitol (MM-mannitol). After a couple of months of Adaptive Laboratory Evolution (ALE) three Saccharomyces cerevisiae diploid strains (YPS606, RM11 and T7) were successfully adapted to grow on MM-mannitol. Despite the efforts, the laboratory CENPK113-7D strain was unable to utilize this sugar alcohol as a carbon source.
Period27 Aug 20171 Sep 2017
Event title28th International Conference on Yeast Genetics and Molecular Biology
Event typeConference
LocationPrague, Czech Republic
Degree of RecognitionInternational