Optimization of Large Volume Injection for Improved Detection of Polycyclic Aromatic Hydrocarbons (PAH) in Mussels

Research output: Contribution to journalJournal article – Annual report year: 2008Researchpeer-review

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Detection of PAH of six benzene rings is somewhat troublesome and lowering the limits of detection (LODs) for these compounds in food is necessary. For this purpose, we optimized a Programmable-Temperature-Vaporisation (PTV) injection with Large Volume Injection (LVI) with regard to the GC-MS detection of anthracene, benz[a]anthracene, benzo[a]pyrene, indeno[1,2,3-cd]pyrene and dibenzo[a,e]pyrene. The optimization of PTV-LVI for GC-MS analysis included the choice of liner, solvent venting, splitless time, split flow and initial inlet temperature for injection of 25 L standard solution and spiked mussel samples. Samples were extracted with Accelerated Solvent Extraction (ASE) followed by two semi-automatic clean-up steps; gel permeation chromatography (GPC) on S-X3 and solid phase extraction (SPE) on pre-packed silica columns, prior to gas chromatography-mass spectrometry (GC-MS) detection. In comparison to traditional splitless injection, LODs were lowered for eighteen PAHs by the use of PTV-LVI ranging from 0.05 g kg-1to 1.0 g kg-1 fresh weight. In particular, the LOD of dibenzo[a,e]pyrene was improved by a factor of ten when using the validated PTV-LVI method.
Original languageEnglish
JournalPolycyclic Aromatic Compounds
Volume28
Issue number4-5
Pages (from-to)282-301
ISSN1040-6638
DOIs
Publication statusPublished - 2008
CitationsWeb of Science® Times Cited: No match on DOI
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