YGFP: a spectral variant of GFP

Publication: Research - peer-reviewJournal article – Annual report year: 2011

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We describe YGFP, a slow bleaching green fluorescent protein (GFP) with unique spectral properties. YGFP is derived from an Escherichia coli codon-optimized synthetic gfp, mutant 2 derivative. In addition to the GFP-mut 2 changes, it also carries S202F and T203I substitutions. YGFP can be used as a substitute for yellow fluorescent protein (YFP) in experiments in which two or more fluorescent proteins are fused to different cellular protein components, expanding the ability to study multiple labeled proteins in a cell at once.
Original languageEnglish
JournalBioTechniques
Publication date2011
Volume50
Journal number6
Pages411-412
ISSN0736-6205
DOIs
StatePublished

Bibliographical note

Supplementary material for this article is available at www.BioTechniques.com/article/113691.

CitationsWeb of Science® Times Cited: 1

Keywords

  • Fluorescent spectra, Fluorescent ParB foci, Photo-bleaching, Multifluorescence cell labeling, mOrange, Fluorescence microscopy
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