DOI

  • Author: Cabon, J.

    ANSES - French Agency for Food, Environmental and Occupational Health & Safety, France

  • Author: Louboutin, L.

    ANSES - French Agency for Food, Environmental and Occupational Health & Safety, France

  • Author: Castric, J.

    ANSES - French Agency for Food, Environmental and Occupational Health & Safety, France

  • Author: Bergmann, S.

    Friedrich-Loeffler-Institute, Germany

  • Author: Bovo, G.

    Instituto Zooprofilattico Sperimentale delle Venezie, Italy

  • Author: Matras, M.

    National Veterinary Research Institute, Poland

  • Author: Haenen, O.

  • Author: Olesen, Niels Jørgen

    Section for Virology, National Veterinary Institute, Technical University of Denmark, Kemitorvet, 2800, Kgs. Lyngby, Denmark

  • Author: Morin, T.

    ANSES - French Agency for Food, Environmental and Occupational Health & Safety, France

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Cyprinid herpesvirus 3 (CyHV-3) is the aetiological agent of a serious infective, notifiable disease affecting common carp and varieties. In survivors, infection is generally characterized by a subclinical latency phase with restricted viral replication. The CyHV-3 genome is difficult to detect in such carrier fish that represent a potential source of dissemination if viral reactivation occurs. In this study, the analytical and diagnostic performance of an alternative serum neutralization (SN) method based on the detection of CyHV-3-specific antibodies was assessed using 151 serum or plasma samples from healthy and naturally or experimentally CyHV-3-infected carp. French CyHV-3 isolate 07/108b was neutralized efficiently by sera from carp infected with European, American and Taiwanese CyHV-3 isolates, but no neutralization was observed using sera specific to other aquatic herpesviruses. Diagnostic sensitivity, diagnostic specificity and repeatability of 95.9%, 99.0% and 99.3%, respectively, were obtained, as well as a compliance rate of 89.9% in reproducibility testing. Neutralizing antibodies were steadily detected in infected carp subjected to restrictive or permissive temperature variations over more than 25 months post-infection. The results suggest that this non-lethal diagnostic test could be used in the future to improve the epidemiological surveillance and control of CyHV-3 disease.
Original languageEnglish
JournalJournal of Fish Diseases
Volume40
Issue number5
Pages (from-to)687-701
ISSN0140-7775
DOIs
StatePublished - 2017
CitationsWeb of Science® Times Cited: 1
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