Use of small diameter column particles to enhance HPLC determination of histamine and other biogenic amines in seafood

Publication: Research - peer-reviewJournal article – Annual report year: 2011

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Pre-column and post-column HPLC derivatization methods were modified and evaluated for the identification and quantification of nine biogenic amines in seafood Two HPLC methods with column particles of 1 8 mu m or 3 mu m in diameter were modified and compared to classical methods using 5 mu m column particles Both pre-column derivatization with dansyl chloride and post-column derivatization with O-phthalaldehyde were studied The HPLC methods were compared with respect to the time of elution eluent consumption backpressure as well as separation sensitivity recovery and repeatability for determination of biogenic amines in lean canned tuna and fatty frozen herring The modified methods using smaller column particles of 1 8 mu m or 3 mu m allowed biogenic amines to be separated and quantified faster (23-59%) and with less eluent consumption (59-62%) than classical HPLC methods Backpressures were below 170 bar and this allowed the use of classical HPLC systems rather than dedicated and costly ultra-high-pressure liquid chromatography (UHPLC) equipment Biogenic amine separation sensitivity recovery and repeatability for the modified methods were similar to or performed better than for the classical HPLC methods Due to reduced analyses time and eluent consumption the modified HPLC methods would improve biogenic amine analysis
Original languageEnglish
JournalLebensmittel - Wissenschaft und Technologie
Publication date2011
Volume44
Journal number2
Pages399-406
ISSN0023-6438
DOIs
StatePublished
CitationsWeb of Science® Times Cited: 6

Keywords

  • Biogenic amines, HPLC columns with small particle size, Histamine
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