Ultrahigh-Field DCE-MRI of Angiogenesis in a Novel Angiogenesis Mouse Model

Publication: Research - peer-reviewJournal article – Annual report year: 2011

Without internal affiliation

Documents

DOI

  • Author: Wittenborn, Thomas

    Aarhus University

  • Author: Nielsen, Thomas

    Aarhus University

  • Author: Nygaard, Jens V.

    Aarhus University

  • Author: Larsen, Esben Kjær Unmack

    Unknown

  • Author: Thim, Troels

    Aarhus University

  • Author: Rydtoft, Louise M.

    Aarhus University

  • Author: Vorup-Jensen, Thomas

    Aarhus University

  • Author: Kjems, Jørgen

    Aarhus University

  • Author: Nielsen, Niels Chr.

    Aarhus University

  • Author: Horsman, Michael R.

    Aarhus University

  • Author: Falk, Erling

    Aarhus University

View graph of relations

Purpose: To be able to screen and identify potential candidate agents for noninvasive imaging of diseases involving angiogenesis, a standardized in vivo angiogenesis model is needed. Angiogenesis is a common feature of many pathological conditions and has become an important target for diagnosis and treatment, with many noninvasive imaging agents emerging. Materials and Methods: Uniform scaffolds consisting of porous and flexible polycaprolactone were implanted subcutaneously in mice and studied after 1 to 6 weeks to describe the time course of angiogenesis. The model was characterized by histology and dynamic contrastenhanced magnetic resonance imaging (DCE-MRI). Results: Microscopic examination revealed progressive ingrowth of new vessels from the periphery, leading to a fully vascularized scaffold within 6 weeks. Blood flow through the new vessels, assessed by DCE-MRI, revealed peripheral vascularization corresponding to 12.3% (SD 6.1%) of scaffold area at week 1 and a more uniform and complete distribution of vessels corresponding to 84.1% (SD 16.2%) of scaffold area at week 4. Conclusion: In agreement with microscopic examination, noninvasive DCE-MRI visualized progressive development of new vessels in a novel and standardized murine angiogenesis model, making this model suitable for screening angiogenesis-related drugs and contrast agents.
Keyword: Scaffold,Angiogenesis,Immunohistochemistry,Dynamic contrast-enhanced magnetic resonance imaging
Original languageEnglish
JournalJournal of Magnetic Resonance Imaging
Publication date2011
ISSN1053-1807
DOIs
StatePublished
CitationsWeb of Science® Times Cited: 5
Download as:
Download as PDF
Select render style:
APAAuthorCBEHarvardMLAStandardVancouverShortLong
PDF
Download as HTML
Select render style:
APAAuthorCBEHarvardMLAStandardVancouverShortLong
HTML
Download as Word
Select render style:
APAAuthorCBEHarvardMLAStandardVancouverShortLong
Word

Download statistics

No data available

ID: 6443010