Transcriptomic analysis of mRNA expression and alternative splicing during mouse sex determination

Publication: Research - peer-reviewJournal article – Annual report year: 2018

DOI

  • Author: Zhao, Liang

    University of Queensland

  • Author: Wang, Chenwei

    Queensland University of Technology

  • Author: Lehman, Melanie L.

    Queensland University of Technology

  • Author: He, Mingyu

    Longsoft

  • Author: An, Jiyuan

    Queensland University of Technology

  • Author: Svingen, Terje

    University of Queensland

    Research Group for Molecular and Reproductive Toxicology, National Food Institute, Technical University of Denmark, Kemitorvet, 2800, Kgs. Lyngby, Denmark

  • Author: Spiller, Cassy M.

    University of Queensland

  • Author: Ng, Ee Ting

    University of Queensland

  • Author: Nelson, Colleen C.

    Queensland University of Technology

  • Author: Koopman, Peter

    University of Queensland

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Mammalian sex determination hinges on sexually dimorphic transcriptional programs in developing fetal gonads. A comprehensive view of these programs is crucial for understanding the normal development of fetal testes and ovaries and the etiology of human disorders of sex development (DSDs), many of which remain unexplained. Using strand-specific RNA-sequencing, we characterized the mouse fetal gonadal transcriptome from 10.5 to 13.5 days post coitum, a key time window in sex determination and gonad development. Our dataset benefits from a greater sensitivity, accuracy and dynamic range compared to microarray studies, allows global dynamics and sex-specificity of gene expression to be assessed, and provides a window to non-transcriptional events such as alternative splicing. Spliceomic analysis uncovered female-specific regulation of Lef1 splicing, which may contribute to the enhanced WNT signaling activity in XX gonads. We provide a user-friendly visualization tool for the complete transcriptomic and spliceomic dataset as a resource for the field.

Original languageEnglish
JournalMolecular and Cellular Endocrinology
Number of pages13
ISSN0303-7207
DOIs
StateAccepted/In press - 2018
CitationsWeb of Science® Times Cited: No match on DOI

    Keywords

  • Gonadal development, Lef1, Ovary, RNA-Seq, Testis, Transcriptional regulation
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