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@article{e83fa2c774eb420bbb66d05caaf8921a,
title = "Transcriptional Portrait of Actinobacillus pleuropneumoniae during Acute Disease - Potential Strategies for Survival and Persistence in the Host",
publisher = "Public Library of Science",
author = "Schou, {Kirstine Klitgaard} and Rundsten, {Carsten Friis} and Jensen, {Tim Kåre} and Øystein Angen and Mette Boye",
note = "This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.",
year = "2012",
doi = "10.1371/journal.pone.0035549",
volume = "7",
number = "4",
pages = "e35549",
journal = "P L o S One",
issn = "1932-6203",

}

RIS

TY - JOUR

T1 - Transcriptional Portrait of Actinobacillus pleuropneumoniae during Acute Disease - Potential Strategies for Survival and Persistence in the Host

A1 - Schou,Kirstine Klitgaard

A1 - Rundsten,Carsten Friis

A1 - Jensen,Tim Kåre

A1 - Angen,Øystein

A1 - Boye,Mette

AU - Schou,Kirstine Klitgaard

AU - Rundsten,Carsten Friis

AU - Jensen,Tim Kåre

AU - Angen,Øystein

AU - Boye,Mette

PB - Public Library of Science

PY - 2012

Y1 - 2012

N2 - <p><strong>Background</strong><br/> Gene expression profiles of bacteria in their natural hosts can provide novel insight into the host-pathogen interactions and molecular determinants of bacterial infections. In the present study, the transcriptional profile of the porcine lung pathogen Actinobacillus pleuropneumoniae was monitored during the acute phase of infection in its natural host.</p> <p><strong>Methodology/Principal Findings</strong><br/> Bacterial expression profiles of A. pleuropneumoniae isolated from lung lesions of 25 infected pigs were compared in samples taken 6, 12, 24 and 48 hours post experimental challenge. Within 6 hours, focal, fibrino hemorrhagic lesions could be observed in the pig lungs, indicating that A. pleuropneumoniae had managed to establish itself successfully in the host. We identified 237 differentially regulated genes likely to encode functions required by the bacteria for colonization and survival in the host. This group was dominated by genes involved in various aspects of energy metabolism, especially anaerobic respiration and carbohydrate metabolism. Remodeling of the bacterial envelope and modifications of posttranslational processing of proteins also appeared to be of importance during early infection. The results suggested that A. pleuropneumoniae is using various strategies to increase its fitness, such as applying Na+ pumps as an alternative way of gaining energy. Furthermore, the transcriptional data provided potential clues as to how A. pleuropneumoniae is able to circumvent host immune factors and survive within the hostile environment of host macrophages. This persistence within macrophages may be related to urease activity, mobilization of various stress responses and active evasion of the host defenses by cell surface sialylation.</p> <p><strong>Conclusions/Significance</strong><br/> The data presented here highlight the importance of metabolic adjustments to host conditions as virulence factors of infecting microorganisms and help to provide insight into the mechanisms behind the efficient colonization and persistence of A. pleuropneumoniae during acute disease.</p>

AB - <p><strong>Background</strong><br/> Gene expression profiles of bacteria in their natural hosts can provide novel insight into the host-pathogen interactions and molecular determinants of bacterial infections. In the present study, the transcriptional profile of the porcine lung pathogen Actinobacillus pleuropneumoniae was monitored during the acute phase of infection in its natural host.</p> <p><strong>Methodology/Principal Findings</strong><br/> Bacterial expression profiles of A. pleuropneumoniae isolated from lung lesions of 25 infected pigs were compared in samples taken 6, 12, 24 and 48 hours post experimental challenge. Within 6 hours, focal, fibrino hemorrhagic lesions could be observed in the pig lungs, indicating that A. pleuropneumoniae had managed to establish itself successfully in the host. We identified 237 differentially regulated genes likely to encode functions required by the bacteria for colonization and survival in the host. This group was dominated by genes involved in various aspects of energy metabolism, especially anaerobic respiration and carbohydrate metabolism. Remodeling of the bacterial envelope and modifications of posttranslational processing of proteins also appeared to be of importance during early infection. The results suggested that A. pleuropneumoniae is using various strategies to increase its fitness, such as applying Na+ pumps as an alternative way of gaining energy. Furthermore, the transcriptional data provided potential clues as to how A. pleuropneumoniae is able to circumvent host immune factors and survive within the hostile environment of host macrophages. This persistence within macrophages may be related to urease activity, mobilization of various stress responses and active evasion of the host defenses by cell surface sialylation.</p> <p><strong>Conclusions/Significance</strong><br/> The data presented here highlight the importance of metabolic adjustments to host conditions as virulence factors of infecting microorganisms and help to provide insight into the mechanisms behind the efficient colonization and persistence of A. pleuropneumoniae during acute disease.</p>

UR - http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0035549

U2 - 10.1371/journal.pone.0035549

DO - 10.1371/journal.pone.0035549

JO - P L o S One

JF - P L o S One

SN - 1932-6203

IS - 4

VL - 7

SP - e35549

ER -