Towards a portable microchip system with integrated thermal control and polymer waveguides for real-time PCR

Publication: Research - peer-reviewJournal article – Annual report year: 2006

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A novel real-time PCR microchip platform with integrated thermal system and polymer waveguides has been developed. The integrated polymer optical system for real-time monitoring of PCR was fabricated in the same SU-8 layer as the PCR chamber, without additional masking steps. Two suitable DNA binding dyes, SYTOX Orange and TO-PRO-3, were selected and tested for the real-time PCR processes. As a model, cadF gene of Campylobacter jejuni has been amplified on the microchip. Using the integrated optical system of the real-time PCR microchip, the measured cycle threshold values of the real-time PCR performed with a dilution series of C. jejuni DNA template (2 to 200 pg/mu l) could be quantitatively detected and compared with a conventional post-PCR analysis (DNA gel electrophoresis). The presented approach provided reliable real-time quantitative information of the PCR amplification of the targeted gene. With the integrated optical system, the reaction dynamics at any location inside the micro reaction chamber can easily be monitored.
Original languageEnglish
JournalElectrophoresis
Publication date2006
Volume27
Journal number24
Pages5051-5058
ISSN0173-0835
DOIs
StatePublished
CitationsWeb of Science® Times Cited: 18

Keywords

  • polymer waveguides, SU-8, real-time PCR, integrated thermal system
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