Time-dependent changes in protein expression in rainbow trout muscle following hypoxia

Publication: Research - peer-reviewJournal article – Annual report year: 2012

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Time-dependent changes in protein expression in rainbow trout muscle following hypoxia. / Wulff, Tune; Jokumsen, Alfred; Højrup, Peter; Jessen, Flemming.

In: Journal of Proteomics, Vol. 75, No. 8, 2012, p. 2342-2351.

Publication: Research - peer-reviewJournal article – Annual report year: 2012

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Author

Wulff, Tune; Jokumsen, Alfred; Højrup, Peter; Jessen, Flemming / Time-dependent changes in protein expression in rainbow trout muscle following hypoxia.

In: Journal of Proteomics, Vol. 75, No. 8, 2012, p. 2342-2351.

Publication: Research - peer-reviewJournal article – Annual report year: 2012

Bibtex

@article{9b5f0fbcb8ee4a4e93929d7a22857c5d,
title = "Time-dependent changes in protein expression in rainbow trout muscle following hypoxia",
keywords = "Aquaculture, Hypoxia, Oxidative stress, Proteomics, Rainbow trout",
publisher = "Elsevier BV",
author = "Tune Wulff and Alfred Jokumsen and Peter Højrup and Flemming Jessen",
year = "2012",
doi = "10.1016/j.jprot.2012.02.010",
volume = "75",
number = "8",
pages = "2342--2351",
journal = "Journal of Proteomics",
issn = "1874-3919",

}

RIS

TY - JOUR

T1 - Time-dependent changes in protein expression in rainbow trout muscle following hypoxia

A1 - Wulff,Tune

A1 - Jokumsen,Alfred

A1 - Højrup,Peter

A1 - Jessen,Flemming

AU - Wulff,Tune

AU - Jokumsen,Alfred

AU - Højrup,Peter

AU - Jessen,Flemming

PB - Elsevier BV

PY - 2012

Y1 - 2012

N2 - Adaptation to hypoxia is a complex process, and individual proteins will be up- or down-regulated in order to address the main challenges at any given time. To investigate the dynamics of the adaptation, rainbow trout (Oncorhynchus mykiss) was exposed to 30% of normal oxygen tension for 1, 2, 5 and 24h respectively, after which muscle samples were taken. The successful investigation of numerous proteins in a single study was achieved by selectively separating the sarcoplasmic proteins using 2-DE. In total 46 protein spots were identified as changing in abundance in response to hypoxia using one-way ANOVA and multivariate data analysis. Proteins of interest were subsequently identified by MS/MS following tryptic digestion. The observed regulation following hypoxia in skeletal muscle was determined to be time specific, as only a limited number of proteins were regulated in response to more than one time point. The cellular response to hypoxia included regulation of proteins involved in maintaining iron homeostasis, energy levels and muscle structure. In conclusion, this proteome-based study presents a comprehensive investigation of the expression profiles of numerous proteins at four different time points. This increases our understanding of timed changes in protein expression in rainbow trout muscle following hypoxia.

AB - Adaptation to hypoxia is a complex process, and individual proteins will be up- or down-regulated in order to address the main challenges at any given time. To investigate the dynamics of the adaptation, rainbow trout (Oncorhynchus mykiss) was exposed to 30% of normal oxygen tension for 1, 2, 5 and 24h respectively, after which muscle samples were taken. The successful investigation of numerous proteins in a single study was achieved by selectively separating the sarcoplasmic proteins using 2-DE. In total 46 protein spots were identified as changing in abundance in response to hypoxia using one-way ANOVA and multivariate data analysis. Proteins of interest were subsequently identified by MS/MS following tryptic digestion. The observed regulation following hypoxia in skeletal muscle was determined to be time specific, as only a limited number of proteins were regulated in response to more than one time point. The cellular response to hypoxia included regulation of proteins involved in maintaining iron homeostasis, energy levels and muscle structure. In conclusion, this proteome-based study presents a comprehensive investigation of the expression profiles of numerous proteins at four different time points. This increases our understanding of timed changes in protein expression in rainbow trout muscle following hypoxia.

KW - Aquaculture

KW - Hypoxia

KW - Oxidative stress

KW - Proteomics

KW - Rainbow trout

U2 - 10.1016/j.jprot.2012.02.010

DO - 10.1016/j.jprot.2012.02.010

JO - Journal of Proteomics

JF - Journal of Proteomics

SN - 1874-3919

IS - 8

VL - 75

SP - 2342

EP - 2351

ER -