The Use of Infrared Thermography as a Novel Approach for Real-Time Validation of PCR Thermocyclers
Publication: Research - peer-review › Journal article – Annual report year: 2010
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The Use of Infrared Thermography as a Novel Approach for Real-Time Validation of PCR Thermocyclers. / Grønlund, Hugo Ahlm; Löfström, Charlotta; Helleskov, Jens Bue; Hoorfar, Jeffrey.
In: Food Analytical Methods, Vol. 3, No. 2, 06.2010, p. 116–119.Publication: Research - peer-review › Journal article – Annual report year: 2010
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TY - JOUR
T1 - The Use of Infrared Thermography as a Novel Approach for Real-Time Validation of PCR Thermocyclers
A1 - Grønlund,Hugo Ahlm
A1 - Löfström,Charlotta
A1 - Helleskov,Jens Bue
A1 - Hoorfar,Jeffrey
AU - Grønlund,Hugo Ahlm
AU - Löfström,Charlotta
AU - Helleskov,Jens Bue
AU - Hoorfar,Jeffrey
PB - Springer New York LLC
PY - 2010/6
Y1 - 2010/6
N2 - Validation of PCR thermocycler performance is crucial to obtain reliable results. In this study, infrared (IR) thermography was evaluated as a novel validation tool.<br/>After stabilisation, no significant difference in the temperatures recorded using thermography and a reference blockbased system was found. By employing IR thermography, information about the length of the time until temperature stabilisation in the sample could be obtained. This study shows the potential of using IR thermography for validation of thermocyclers.
AB - Validation of PCR thermocycler performance is crucial to obtain reliable results. In this study, infrared (IR) thermography was evaluated as a novel validation tool.<br/>After stabilisation, no significant difference in the temperatures recorded using thermography and a reference blockbased system was found. By employing IR thermography, information about the length of the time until temperature stabilisation in the sample could be obtained. This study shows the potential of using IR thermography for validation of thermocyclers.
KW - PCR
KW - Thermocycler
KW - Thermal Cycler
KW - Infrared Thermography
KW - Validation
U2 - 10.1007/s12161-009-9103-2
DO - 10.1007/s12161-009-9103-2
JO - Food Analytical Methods
JF - Food Analytical Methods
SN - 1936-9751
IS - 2
VL - 3
SP - 116
EP - 119
ER -