The transcriptionally active regions in the genome of Bacillus subtilis

Publication: Research - peer-reviewJournal article – Annual report year: 2009

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The transcriptionally active regions in the genome of Bacillus subtilis. / Rasmussen, Simon; Nielsen, Henrik Bjørn; Jarmer, Hanne Østergaard.

In: Molecular Microbiology, Vol. 17, No. 19, 2009, p. 1043-1057.

Publication: Research - peer-reviewJournal article – Annual report year: 2009

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Rasmussen, Simon; Nielsen, Henrik Bjørn; Jarmer, Hanne Østergaard / The transcriptionally active regions in the genome of Bacillus subtilis.

In: Molecular Microbiology, Vol. 17, No. 19, 2009, p. 1043-1057.

Publication: Research - peer-reviewJournal article – Annual report year: 2009

Bibtex

@article{910e6628bb2c46b696a67f9691088db7,
title = "The transcriptionally active regions in the genome of Bacillus subtilis",
publisher = "Wiley-Blackwell Publishing Ltd.",
author = "Simon Rasmussen and Nielsen, {Henrik Bjørn} and Jarmer, {Hanne Østergaard}",
year = "2009",
doi = "10.1111/j.1365-2958.2009.06830.x",
volume = "17",
number = "19",
pages = "1043--1057",
journal = "Molecular Microbiology",
issn = "0950-382X",

}

RIS

TY - JOUR

T1 - The transcriptionally active regions in the genome of Bacillus subtilis

A1 - Rasmussen,Simon

A1 - Nielsen,Henrik Bjørn

A1 - Jarmer,Hanne Østergaard

AU - Rasmussen,Simon

AU - Nielsen,Henrik Bjørn

AU - Jarmer,Hanne Østergaard

PB - Wiley-Blackwell Publishing Ltd.

PY - 2009

Y1 - 2009

N2 - The majority of all genes have so far been identified and annotated systematically through in silico gene finding. Here we report the finding of 3662 strand-specific transcriptionally active regions (TARs) in the genome of Bacillus subtilis by the use of tiling arrays. We have measured the genome-wide expression during mid-exponential growth on rich (LB) and minimal (M9) medium. The identified TARs account for 77.3% of the genes as they are currently annotated and additionally we find 84 putative non-coding RNAs (ncRNAs) and 127 antisense transcripts. One ncRNA, ncr22, is predicted to act as a translational control on cstA and an antisense transcript was observed opposite the housekeeping sigma factor sigA. Through this work we have discovered a long conserved 3' untranslated region (UTR) in a group of membrane-associated genes that is predicted to fold into a large and highly stable secondary structure. One of the genes having this tail is efeN, which encodes a target of the twin-arginine translocase (Tat) protein translocation system.

AB - The majority of all genes have so far been identified and annotated systematically through in silico gene finding. Here we report the finding of 3662 strand-specific transcriptionally active regions (TARs) in the genome of Bacillus subtilis by the use of tiling arrays. We have measured the genome-wide expression during mid-exponential growth on rich (LB) and minimal (M9) medium. The identified TARs account for 77.3% of the genes as they are currently annotated and additionally we find 84 putative non-coding RNAs (ncRNAs) and 127 antisense transcripts. One ncRNA, ncr22, is predicted to act as a translational control on cstA and an antisense transcript was observed opposite the housekeeping sigma factor sigA. Through this work we have discovered a long conserved 3' untranslated region (UTR) in a group of membrane-associated genes that is predicted to fold into a large and highly stable secondary structure. One of the genes having this tail is efeN, which encodes a target of the twin-arginine translocase (Tat) protein translocation system.

U2 - 10.1111/j.1365-2958.2009.06830.x

DO - 10.1111/j.1365-2958.2009.06830.x

JO - Molecular Microbiology

JF - Molecular Microbiology

SN - 0950-382X

IS - 19

VL - 17

SP - 1043

EP - 1057

ER -