The patchwork nature of rolling-circle plasmids: comparison of six plasmids from two distinct Bacillus thuringiensis serotypes

Publication: Research - peer-reviewJournal article – Annual report year: 2003

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The patchwork nature of rolling-circle plasmids: comparison of six plasmids from two distinct Bacillus thuringiensis serotypes. / Andrup, Lars; Jensen, Gert Bolander; Wilcks, Andrea; Ørum-Smidt, Lasse; Hoflack, Lieve; Mahillon, Jacques.

In: Plasmid, Vol. 49, No. 3, 2003, p. 205-232.

Publication: Research - peer-reviewJournal article – Annual report year: 2003

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Andrup, Lars; Jensen, Gert Bolander; Wilcks, Andrea; Ørum-Smidt, Lasse; Hoflack, Lieve; Mahillon, Jacques / The patchwork nature of rolling-circle plasmids: comparison of six plasmids from two distinct Bacillus thuringiensis serotypes.

In: Plasmid, Vol. 49, No. 3, 2003, p. 205-232.

Publication: Research - peer-reviewJournal article – Annual report year: 2003

Bibtex

@article{f1ab35bb8db44162a2694e22685e92a5,
title = "The patchwork nature of rolling-circle plasmids: comparison of six plasmids from two distinct Bacillus thuringiensis serotypes",
publisher = "Academic Press",
author = "Lars Andrup and Jensen, {Gert Bolander} and Andrea Wilcks and Lasse Ørum-Smidt and Lieve Hoflack and Jacques Mahillon",
year = "2003",
doi = "10.1016/S0147-619X(03)00015-5",
volume = "49",
number = "3",
pages = "205--232",
journal = "Plasmid",
issn = "0147-619X",

}

RIS

TY - JOUR

T1 - The patchwork nature of rolling-circle plasmids: comparison of six plasmids from two distinct Bacillus thuringiensis serotypes

A1 - Andrup,Lars

A1 - Jensen,Gert Bolander

A1 - Wilcks,Andrea

A1 - Ørum-Smidt,Lasse

A1 - Hoflack,Lieve

A1 - Mahillon,Jacques

AU - Andrup,Lars

AU - Jensen,Gert Bolander

AU - Wilcks,Andrea

AU - Ørum-Smidt,Lasse

AU - Hoflack,Lieve

AU - Mahillon,Jacques

PB - Academic Press

PY - 2003

Y1 - 2003

N2 - Bacillus thuringiensis, the entomopathogenic bacteria from the Bacillus cereus group, harbors numerous extrachromosomal molecules whose sizes vary from 2 to more than 200 kb. Apart from the genes coding for the biopesticide delta-endotoxins located on large plasmids, little information has been obtained on these plasmids and their contribution to the biology of their host. In this paper, we embarked on a detailed comparison of six small rolling-circle replicating (RCR) plasmids originating from two major B. thuringiensis strains. The complete nucleotide sequences of plasmid pGIl, pGI2, pGI3, pTX14-1, pTX14-2, and pTX14-3 have been obtained and compared. Replication functions, comprising, for each plasmid, the gene encoding the Rep-protein, double-strand origin of replication (dso), single-strand origin of replication (sso), have been identified and analyzed. Two new families, or homology groups, of RCR plasmids originated from the studies of these plasmids (Group VI based on pGI3 and Group VII based on pTX14-3). On five of the six plasmids, loci involved in conjugative mobilization (Mob-genes and origin of transfer (oriT)) were identified. Plasmids pTX14-1, pTX14-2, and pTX14-3 each harbor an ORF encoding a polypeptide containing a central domain with repetitive elements similar to eukaryotic collagen (Gly-X-Y triplets). These genes were termed bcol for Bacillus-collagen-like genes. (C) 2003 Elsevier Science (USA). All rights reserved.

AB - Bacillus thuringiensis, the entomopathogenic bacteria from the Bacillus cereus group, harbors numerous extrachromosomal molecules whose sizes vary from 2 to more than 200 kb. Apart from the genes coding for the biopesticide delta-endotoxins located on large plasmids, little information has been obtained on these plasmids and their contribution to the biology of their host. In this paper, we embarked on a detailed comparison of six small rolling-circle replicating (RCR) plasmids originating from two major B. thuringiensis strains. The complete nucleotide sequences of plasmid pGIl, pGI2, pGI3, pTX14-1, pTX14-2, and pTX14-3 have been obtained and compared. Replication functions, comprising, for each plasmid, the gene encoding the Rep-protein, double-strand origin of replication (dso), single-strand origin of replication (sso), have been identified and analyzed. Two new families, or homology groups, of RCR plasmids originated from the studies of these plasmids (Group VI based on pGI3 and Group VII based on pTX14-3). On five of the six plasmids, loci involved in conjugative mobilization (Mob-genes and origin of transfer (oriT)) were identified. Plasmids pTX14-1, pTX14-2, and pTX14-3 each harbor an ORF encoding a polypeptide containing a central domain with repetitive elements similar to eukaryotic collagen (Gly-X-Y triplets). These genes were termed bcol for Bacillus-collagen-like genes. (C) 2003 Elsevier Science (USA). All rights reserved.

KW - Plasmids

KW - Evolution

KW - DNA sequence

KW - Rolling-circle replication

KW - Horizontal DNA transfer

KW - Bacillus thuringiensis

KW - Recombination

KW - Transposon

KW - Collagen-like proteins

KW - Functional modules

KW - Mobile elements

U2 - 10.1016/S0147-619X(03)00015-5

DO - 10.1016/S0147-619X(03)00015-5

JO - Plasmid

JF - Plasmid

SN - 0147-619X

IS - 3

VL - 49

SP - 205

EP - 232

ER -