The patchwork nature of rolling-circle plasmids: comparison of six plasmids from two distinct Bacillus thuringiensis serotypes

Publication: Research - peer-reviewJournal article – Annual report year: 2003

Without internal affiliation

  • Author: Andrup, Lars

    National Institute of Occupational Health, Denmark

  • Author: Jensen, Gert Bolander

    National Institute of Occupational Health, Denmark

  • Author: Wilcks, Andrea

    National Institute of Occupational Health

  • Author: Ørum-Smidt, Lasse

    National Institute of Occupational Health

  • Author: Hoflack, Lieve

    Laboratory of Food and Environmental Microbiology, Belgium

  • Author: Mahillon, Jacques

    Laboratory of Food and Environmental Microbiology, Belgium

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Bacillus thuringiensis, the entomopathogenic bacteria from the Bacillus cereus group, harbors numerous extrachromosomal molecules whose sizes vary from 2 to more than 200 kb. Apart from the genes coding for the biopesticide delta-endotoxins located on large plasmids, little information has been obtained on these plasmids and their contribution to the biology of their host. In this paper, we embarked on a detailed comparison of six small rolling-circle replicating (RCR) plasmids originating from two major B. thuringiensis strains. The complete nucleotide sequences of plasmid pGIl, pGI2, pGI3, pTX14-1, pTX14-2, and pTX14-3 have been obtained and compared. Replication functions, comprising, for each plasmid, the gene encoding the Rep-protein, double-strand origin of replication (dso), single-strand origin of replication (sso), have been identified and analyzed. Two new families, or homology groups, of RCR plasmids originated from the studies of these plasmids (Group VI based on pGI3 and Group VII based on pTX14-3). On five of the six plasmids, loci involved in conjugative mobilization (Mob-genes and origin of transfer (oriT)) were identified. Plasmids pTX14-1, pTX14-2, and pTX14-3 each harbor an ORF encoding a polypeptide containing a central domain with repetitive elements similar to eukaryotic collagen (Gly-X-Y triplets). These genes were termed bcol for Bacillus-collagen-like genes. (C) 2003 Elsevier Science (USA). All rights reserved.
Original languageEnglish
JournalPlasmid
Publication date2003
Volume49
Issue3
Pages205-232
ISSN0147-619X
DOIs
StatePublished
CitationsWeb of Science® Times Cited: 35

Keywords

  • Plasmids, Evolution, DNA sequence, Rolling-circle replication, Horizontal DNA transfer, Bacillus thuringiensis, Recombination, Transposon, Collagen-like proteins, Functional modules, Mobile elements
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