The glycolytic flux in Escherichia coli is controlled by the demand for ATP

Publication: Research - peer-reviewJournal article – Annual report year: 2002

Standard

The glycolytic flux in Escherichia coli is controlled by the demand for ATP. / Købmann, Brian Jensen; Westerhoff, H.V.; Snoep, J.L.; Nilsson, D.; Jensen, Peter Ruhdal.

In: Journal of bacteriology, Vol. 184, No. 14, 2002, p. 3909-3916.

Publication: Research - peer-reviewJournal article – Annual report year: 2002

Harvard

Købmann, BJ, Westerhoff, HV, Snoep, JL, Nilsson, D & Jensen, PR 2002, 'The glycolytic flux in Escherichia coli is controlled by the demand for ATP' Journal of bacteriology, vol 184, no. 14, pp. 3909-3916.

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Author

Købmann, Brian Jensen; Westerhoff, H.V.; Snoep, J.L.; Nilsson, D.; Jensen, Peter Ruhdal / The glycolytic flux in Escherichia coli is controlled by the demand for ATP.

In: Journal of bacteriology, Vol. 184, No. 14, 2002, p. 3909-3916.

Publication: Research - peer-reviewJournal article – Annual report year: 2002

Bibtex

@article{a4ed4ca0e0fc4f7293420d3f8a5dc4a7,
title = "The glycolytic flux in Escherichia coli is controlled by the demand for ATP",
publisher = "American Society for Microbiology",
author = "Købmann, {Brian Jensen} and H.V. Westerhoff and J.L. Snoep and D. Nilsson and Jensen, {Peter Ruhdal}",
year = "2002",
volume = "184",
number = "14",
pages = "3909--3916",
journal = "Journal of bacteriology",
issn = "0021-9193",

}

RIS

TY - JOUR

T1 - The glycolytic flux in Escherichia coli is controlled by the demand for ATP

A1 - Købmann,Brian Jensen

A1 - Westerhoff,H.V.

A1 - Snoep,J.L.

A1 - Nilsson,D.

A1 - Jensen,Peter Ruhdal

AU - Købmann,Brian Jensen

AU - Westerhoff,H.V.

AU - Snoep,J.L.

AU - Nilsson,D.

AU - Jensen,Peter Ruhdal

PB - American Society for Microbiology

PY - 2002

Y1 - 2002

N2 - The nature of the control of glycolytic flux is one of the central, as-yet-uncharacterized issues in cellular metabolism. We developed a molecular genetic tool that specifically induces ATP hydrolysis in living cells without interfering with other aspects of metabolism. Genes encoding the F-1 part of the membrane-bound (F1F0) H+-ATP synthase were expressed in steadily growing Escherichia coli cells, which lowered the intracellular [ATP]/[ADP] ratio. This resulted in a strong stimulation of the specific glycolytic flux concomitant with a smaller decrease in the growth rate of the cells. By optimizing additional ATP hydrolysis, we increased the flux through glycolysis to 1.7 times that of the wild-type flux. The results demonstrate why attempts in the past to increase the glycolytic flux through overexpression of glycolytic enzymes have been unsuccessful: the majority of flux control (> 75%) resides not inside but outside the pathway, i.e., with the enzymes that hydrolyze ATP. These data further allowed us to answer the question of whether catabolic or anabolic reactions control the growth of E. coli. We show that the majority of the control of growth rate resides in the anabolic reactions, i.e., the cells are mostly "carbon" limited. Ways to increase the efficiency and productivity of industrial fermentation processes are discussed.

AB - The nature of the control of glycolytic flux is one of the central, as-yet-uncharacterized issues in cellular metabolism. We developed a molecular genetic tool that specifically induces ATP hydrolysis in living cells without interfering with other aspects of metabolism. Genes encoding the F-1 part of the membrane-bound (F1F0) H+-ATP synthase were expressed in steadily growing Escherichia coli cells, which lowered the intracellular [ATP]/[ADP] ratio. This resulted in a strong stimulation of the specific glycolytic flux concomitant with a smaller decrease in the growth rate of the cells. By optimizing additional ATP hydrolysis, we increased the flux through glycolysis to 1.7 times that of the wild-type flux. The results demonstrate why attempts in the past to increase the glycolytic flux through overexpression of glycolytic enzymes have been unsuccessful: the majority of flux control (> 75%) resides not inside but outside the pathway, i.e., with the enzymes that hydrolyze ATP. These data further allowed us to answer the question of whether catabolic or anabolic reactions control the growth of E. coli. We show that the majority of the control of growth rate resides in the anabolic reactions, i.e., the cells are mostly "carbon" limited. Ways to increase the efficiency and productivity of industrial fermentation processes are discussed.

JO - Journal of bacteriology

JF - Journal of bacteriology

SN - 0021-9193

IS - 14

VL - 184

SP - 3909

EP - 3916

ER -