The genomic sequence of the Chinese hamster ovary (CHO)-K1 cell line

Publication: Research - peer-reviewJournal article – Annual report year: 2011

  • Author: Xu, Xun

    BGI-Shenzhen

  • Author: Pan, Shengkai

    BGI-Shenzhen

  • Author: Liu, Xin

    BGI-Shenzhen

  • Author: Chen, Wenbin

    BGI-Shenzhen

  • Author: Xie, Min

    BGI-Shenzhen

  • Author: Wang, Wenliang

    BGI-Shenzhen

  • Author: Wang, Jun

    BGI-Shenzhen

  • Author: Nagarajan, Harish

    GT Life Sciences

  • Author: Lewis, Nathan E.

    GT Life Sciences

  • Author: Famili, Iman

    GT Life Sciences

  • Author: Palsson, Bernhard O.

    GT Life Sciences

  • Author: Cai, Zhiming

    Peking University Shenzhen Hospital, Guangdong Key Laboratory of Male Reproductive Medicine and Genetics

  • Author: Gui, Yaoting

    Peking University Shenzhen Hospital, Guangdong Key Laboratory of Male Reproductive Medicine and Genetics

  • Author: Hammond, Stephanie

    University of Delaware, Department of Chemical Engineering and Delaware Biotechnology Institute

  • Author: Lee, Kelvin H.

    University of Delaware, Department of Chemical Engineering and Delaware Biotechnology Institute

  • Author: Andersen, Mikael Rørdam

    Center for Microbial Biotechnology, Department of Systems Biology, Technical University of Denmark, CMB Søltofts Plads, 2800, Kgs. Lyngby, Denmark

  • Author: Neff, Norma

    Stanford University and Howard Hughes Medical Institute, Department of Bioengineering

  • Author: Passarelli, Benedetto

    Stanford University and Howard Hughes Medical Institute, Department of Bioengineering

  • Author: Koh, Winston

    Stanford University and Howard Hughes Medical Institute, Department of Bioengineering

  • Author: Fan, H. Christina

    Stanford University and Howard Hughes Medical Institute, Department of Bioengineering

  • Author: Wang, Jianbin

    Stanford University and Howard Hughes Medical Institute, Department of Bioengineering

  • Author: Quake, Stephen R.

    Stanford University and Howard Hughes Medical Institute, Department of Bioengineering

  • Author: Betenbaugh, Michael

    Department of Systems Biology, Technical University of Denmark, Søltofts Plads, DK-2800, Kgs. Lyngby, Denmark

  • Author: Palsson, Bernhard

    Network Reconstructions and in silico Biology, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Denmark

  • Author: Wang, Jun

    BGI-Shenzhen

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Chinese hamster ovary (CHO)-derived cell lines are the preferred host cells for the production of therapeutic proteins. Here we present a draft genomic sequence of the CHO-K1 ancestral cell line. The assembly comprises 2.45 Gb of genomic sequence, with 24,383 predicted genes. We associate most of the assembled scaffolds with 21 chromosomes isolated by microfluidics to identify chromosomal locations of genes. Furthermore, we investigate genes involved in glycosylation, which affect therapeutic protein quality, and viral susceptibility genes, which are relevant to cell engineering and regulatory concerns. Homologs of most human glycosylation-associated genes are present in the CHO-K1 genome, although 141 of these homologs are not expressed under exponential growth conditions. Many important viral entry genes are also present in the genome but not expressed, which may explain the unusual viral resistance property of CHO cell lines. We discuss how the availability of this genome sequence may facilitate genome-scale science for the optimization of biopharmaceutical protein production. © 2011 Nature America, Inc. All rights reserved.
Original languageEnglish
JournalNature Biotechnology
Publication date2011
Volume29
Issue8
Pages735-741
ISSN1087-0156
DOIs
StatePublished
CitationsWeb of Science® Times Cited: 103
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