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@article{789b8c10104e4116a47890fc52c05f8d,
title = "The comparative utility of oral swabs and probang samples for detection of foot-and-mouth disease virus infection in cattle and pigs",
publisher = "Elsevier BV",
author = "Stenfeldt, {Anna Carolina} and Louise Lohse and Graham Belsham",
year = "2013",
doi = "10.1016/j.vetmic.2012.09.008",
volume = "162",
number = "2-4",
pages = "330--337",
journal = "Veterinary Microbiology",
issn = "0378-1135",

}

RIS

TY - JOUR

T1 - The comparative utility of oral swabs and probang samples for detection of foot-and-mouth disease virus infection in cattle and pigs

A1 - Stenfeldt,Anna Carolina

A1 - Lohse,Louise

A1 - Belsham,Graham

AU - Stenfeldt,Anna Carolina

AU - Lohse,Louise

AU - Belsham,Graham

PB - Elsevier BV

PY - 2013

Y1 - 2013

N2 - Foot-and-mouth disease virus (FMDV) RNA was measured using quantitative reverse transcription-PCR (qRT-PCR) assays in oralswab and probangsamples collected from cattle and pigs during experimental infections with serotype O FMDV. During acute infection, FMDV RNA was measurable in oralswabs as well as in probangsamples from both species. FMDV RNA could be detected in oralswabs and probangsamples from a time point corresponding to the onset of viremia in directly inoculated animals, whereas animals which were infected through contact exposure had low levels of FMDV RNA in oralswabs before viral RNA could be measured in serum. Analysis of samples collected from cattle persistently infected with FMDV showed that it was not possible to detect FMDV RNA in oralswabs harvested beyond 10 days post infection (dpi), despite the presence of FMDV RNA in probangsamples that had been collected as late as 35 dpi. An interesting feature of the persistent infection in the cattle was the apparent decline in the level of FMDV RNA in probangsamples after the acute phase of infection, which was followed by a marked rise again (in all the carrier animals) by 28 dpi.<br/><br/>Results from this study indicate that qRT-PCR analysis of oralswabs is a useful approach in order to achieve a time efficient and reliable initial diagnosis of acute FMD in cattle and pigs, whereas probang sampling is essential for the detection of cattle that are persistently infected “carriers” of FMDV.

AB - Foot-and-mouth disease virus (FMDV) RNA was measured using quantitative reverse transcription-PCR (qRT-PCR) assays in oralswab and probangsamples collected from cattle and pigs during experimental infections with serotype O FMDV. During acute infection, FMDV RNA was measurable in oralswabs as well as in probangsamples from both species. FMDV RNA could be detected in oralswabs and probangsamples from a time point corresponding to the onset of viremia in directly inoculated animals, whereas animals which were infected through contact exposure had low levels of FMDV RNA in oralswabs before viral RNA could be measured in serum. Analysis of samples collected from cattle persistently infected with FMDV showed that it was not possible to detect FMDV RNA in oralswabs harvested beyond 10 days post infection (dpi), despite the presence of FMDV RNA in probangsamples that had been collected as late as 35 dpi. An interesting feature of the persistent infection in the cattle was the apparent decline in the level of FMDV RNA in probangsamples after the acute phase of infection, which was followed by a marked rise again (in all the carrier animals) by 28 dpi.<br/><br/>Results from this study indicate that qRT-PCR analysis of oralswabs is a useful approach in order to achieve a time efficient and reliable initial diagnosis of acute FMD in cattle and pigs, whereas probang sampling is essential for the detection of cattle that are persistently infected “carriers” of FMDV.

KW - Foot-and-mouth disease

KW - Virus

KW - qRT-PCR

KW - FMDV persistence

U2 - 10.1016/j.vetmic.2012.09.008

DO - 10.1016/j.vetmic.2012.09.008

JO - Veterinary Microbiology

JF - Veterinary Microbiology

SN - 0378-1135

IS - 2-4

VL - 162

SP - 330

EP - 337

ER -