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@article{636d6f52536649db97b5efa11a490c5e,
title = "The challenges of testing metal and metal oxide nanoparticles in algal bioassays: titanium dioxide and gold nanoparticles as case studies",
keywords = "TiO2, Au, Growth inhibition, Ecotoxicity, Guidelines",
publisher = "Informa Healthcare",
author = "Hartmann, {Nanna Isabella Bloch} and Christian Engelbrekt and Jingdong Zhang and Jens Ulstrup and Kusk, {Kresten Ole} and Anders Baun",
year = "2013",
doi = "10.3109/17435390.2012.710657",
volume = "7",
number = "6",
pages = "1082--1094",
journal = "Nanotoxicology",
issn = "1743-5390",

}

RIS

TY - JOUR

T1 - The challenges of testing metal and metal oxide nanoparticles in algal bioassays: titanium dioxide and gold nanoparticles as case studies

A1 - Hartmann,Nanna Isabella Bloch

A1 - Engelbrekt,Christian

A1 - Zhang,Jingdong

A1 - Ulstrup,Jens

A1 - Kusk,Kresten Ole

A1 - Baun,Anders

AU - Hartmann,Nanna Isabella Bloch

AU - Engelbrekt,Christian

AU - Zhang,Jingdong

AU - Ulstrup,Jens

AU - Kusk,Kresten Ole

AU - Baun,Anders

PB - Informa Healthcare

PY - 2013

Y1 - 2013

N2 - Aquatic toxicology of engineered nanoparticles is challenged by methodological difficulties stemming partly from highly dynamic and poorly understood behavior of nanoparticles in biological test systems. In this paper scientific and technical challenges of testing not readily soluble nanoparticles in standardised algal growth inhibition tests are highlighted with specific focus on biomass quantification methods. This is illustrated through tests with TiO(2) and Au nanoparticles, for which cell-nanoparticle interactions and behavior was studied during incubation. Au NP coating layers changed over time and TiO(2) nanoparticle aggregation/agglomeration increased as a function of concentration. Three biomass surrogate measuring techniques were evaluated (coulter counting, cell counting in haemocytometer, and fluorescence of pigment extracts) and out of these the fluorometric methods was found to be most suitable. Background correction was identified as a key issue for biomass quantification, complicated by algae-particle interactions and nanoparticle transformation. Optimisation of the method is needed to reduce further particle interference on measurements.

AB - Aquatic toxicology of engineered nanoparticles is challenged by methodological difficulties stemming partly from highly dynamic and poorly understood behavior of nanoparticles in biological test systems. In this paper scientific and technical challenges of testing not readily soluble nanoparticles in standardised algal growth inhibition tests are highlighted with specific focus on biomass quantification methods. This is illustrated through tests with TiO(2) and Au nanoparticles, for which cell-nanoparticle interactions and behavior was studied during incubation. Au NP coating layers changed over time and TiO(2) nanoparticle aggregation/agglomeration increased as a function of concentration. Three biomass surrogate measuring techniques were evaluated (coulter counting, cell counting in haemocytometer, and fluorescence of pigment extracts) and out of these the fluorometric methods was found to be most suitable. Background correction was identified as a key issue for biomass quantification, complicated by algae-particle interactions and nanoparticle transformation. Optimisation of the method is needed to reduce further particle interference on measurements.

KW - TiO2

KW - Au

KW - Growth inhibition

KW - Ecotoxicity

KW - Guidelines

U2 - 10.3109/17435390.2012.710657

DO - 10.3109/17435390.2012.710657

JO - Nanotoxicology

JF - Nanotoxicology

SN - 1743-5390

IS - 6

VL - 7

SP - 1082

EP - 1094

ER -