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Studying the ability of Fusarium oxysporum and recombinant Saccharomyces cerevisiae to efficiently cooperate in decomposition and ethanolic fermentation of wheat straw. / Panagiotou, Gianni; Topakas, Evangelos; Moukouli, Maria; Christakopoulos, Paul; Olsson, Lisbeth.

In: Biomass & Bioenergy, Vol. 35, No. 8, 2011, p. 3727-3732.

Publication: Research - peer-reviewJournal article – Annual report year: 2011

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Panagiotou, Gianni; Topakas, Evangelos; Moukouli, Maria; Christakopoulos, Paul; Olsson, Lisbeth / Studying the ability of Fusarium oxysporum and recombinant Saccharomyces cerevisiae to efficiently cooperate in decomposition and ethanolic fermentation of wheat straw.

In: Biomass & Bioenergy, Vol. 35, No. 8, 2011, p. 3727-3732.

Publication: Research - peer-reviewJournal article – Annual report year: 2011

Bibtex

@article{f4560630ffb4411ab1467b29e00c4603,
title = "Studying the ability of Fusarium oxysporum and recombinant Saccharomyces cerevisiae to efficiently cooperate in decomposition and ethanolic fermentation of wheat straw",
keywords = "Consolidated bioprocessing, Fusarium oxysporum, Wheat straw, Ethanol, Saccharomyces cerevisiae",
publisher = "Pergamon",
author = "Gianni Panagiotou and Evangelos Topakas and Maria Moukouli and Paul Christakopoulos and Lisbeth Olsson",
year = "2011",
doi = "10.1016/j.biombioe.2011.05.005",
volume = "35",
number = "8",
pages = "3727--3732",
journal = "Biomass & Bioenergy",
issn = "0961-9534",

}

RIS

TY - JOUR

T1 - Studying the ability of Fusarium oxysporum and recombinant Saccharomyces cerevisiae to efficiently cooperate in decomposition and ethanolic fermentation of wheat straw

A1 - Panagiotou,Gianni

A1 - Topakas,Evangelos

A1 - Moukouli,Maria

A1 - Christakopoulos,Paul

A1 - Olsson,Lisbeth

AU - Panagiotou,Gianni

AU - Topakas,Evangelos

AU - Moukouli,Maria

AU - Christakopoulos,Paul

AU - Olsson,Lisbeth

PB - Pergamon

PY - 2011

Y1 - 2011

N2 - Fusarium oxysporum F3 alone or in mixed culture with Saccharomyces cerevisiae F12 were used to ferment carbohydrates of wet exploded pre-treated wheat straw (PWS) directly to ethanol. Both microorganisms were first grown aerobically to produce cell mass and thereafter fermented PWS to ethanol under anaerobic conditions. During fermentation, soluble and insoluble carbohydrates were hydrolysed by the lignocellulolytic system of F. oxysporum. Mixed substrate fermentation using PWS and corn cobs (CC) in the ratio 1:2 was used to obtain an enzyme mixture with high cellulolytic and hemicellulolytic activities. Under these conditions, activities as high as 34300, 9100, 326, 24, 169, 27 and 254 U dm−3 of xylanase, endoglucanase, β-glucosidase, arabinofuranosidase, avicelase, feruloyl esterase and acetyl esterase, respectively, were obtained. The replacement of the enzyme production phase of F. oxysporum by the addition of commercially available enzymes Celluclast® 1.5 L FG and Novozym® 188 in 3:1 ratio for the treatment of PWS, resulted in a 3-fold increase in the volumetric ethanol productivity without increasing the ethanol production significantly. By direct bioconversion of 110 kg m−3 dry matter of PWS, ethanol concentration (4.9 kg m−3) and yield (40 g kg−1 of PWS) were similarly obtained by F. oxysporum and the mixed culture, while productivity rates as high as 34 g m−3 h−1 and 108 g m−3 h−1 were obtained by F. oxysporum and the mixed culture, respectively.

AB - Fusarium oxysporum F3 alone or in mixed culture with Saccharomyces cerevisiae F12 were used to ferment carbohydrates of wet exploded pre-treated wheat straw (PWS) directly to ethanol. Both microorganisms were first grown aerobically to produce cell mass and thereafter fermented PWS to ethanol under anaerobic conditions. During fermentation, soluble and insoluble carbohydrates were hydrolysed by the lignocellulolytic system of F. oxysporum. Mixed substrate fermentation using PWS and corn cobs (CC) in the ratio 1:2 was used to obtain an enzyme mixture with high cellulolytic and hemicellulolytic activities. Under these conditions, activities as high as 34300, 9100, 326, 24, 169, 27 and 254 U dm−3 of xylanase, endoglucanase, β-glucosidase, arabinofuranosidase, avicelase, feruloyl esterase and acetyl esterase, respectively, were obtained. The replacement of the enzyme production phase of F. oxysporum by the addition of commercially available enzymes Celluclast® 1.5 L FG and Novozym® 188 in 3:1 ratio for the treatment of PWS, resulted in a 3-fold increase in the volumetric ethanol productivity without increasing the ethanol production significantly. By direct bioconversion of 110 kg m−3 dry matter of PWS, ethanol concentration (4.9 kg m−3) and yield (40 g kg−1 of PWS) were similarly obtained by F. oxysporum and the mixed culture, while productivity rates as high as 34 g m−3 h−1 and 108 g m−3 h−1 were obtained by F. oxysporum and the mixed culture, respectively.

KW - Consolidated bioprocessing

KW - Fusarium oxysporum

KW - Wheat straw

KW - Ethanol

KW - Saccharomyces cerevisiae

U2 - 10.1016/j.biombioe.2011.05.005

DO - 10.1016/j.biombioe.2011.05.005

JO - Biomass & Bioenergy

JF - Biomass & Bioenergy

SN - 0961-9534

IS - 8

VL - 35

SP - 3727

EP - 3732

ER -