Study of cancer cell lines with Fourier transform infrared (FTIR)/vibrational absorption (VA) spectroscopy

Publication: Research - peer-reviewJournal article – Annual report year: 2013

Standard

Study of cancer cell lines with Fourier transform infrared (FTIR)/vibrational absorption (VA) spectroscopy. / Uceda Otero, E. P. ; Eliel, G. S. N. ; Fonseca, E. J. S. ; Hickmann, J. M. ; Rodarte, R. ; Barreto, E.; Jalkanen, Karl J.

In: Current Physical Chemistry, Vol. 3, No. 1, 2013, p. 36-43.

Publication: Research - peer-reviewJournal article – Annual report year: 2013

Harvard

Uceda Otero, EP, Eliel, GSN, Fonseca, EJS, Hickmann, JM, Rodarte, R, Barreto, E & Jalkanen, KJ 2013, 'Study of cancer cell lines with Fourier transform infrared (FTIR)/vibrational absorption (VA) spectroscopy' Current Physical Chemistry, vol 3, no. 1, pp. 36-43.

APA

Uceda Otero, E. P., Eliel, G. S. N., Fonseca, E. J. S., Hickmann, J. M., Rodarte, R., Barreto, E., & Jalkanen, K. J. (2013). Study of cancer cell lines with Fourier transform infrared (FTIR)/vibrational absorption (VA) spectroscopy. Current Physical Chemistry, 3(1), 36-43.

CBE

MLA

Vancouver

Uceda Otero EP, Eliel GSN, Fonseca EJS, Hickmann JM, Rodarte R, Barreto E et al. Study of cancer cell lines with Fourier transform infrared (FTIR)/vibrational absorption (VA) spectroscopy. Current Physical Chemistry. 2013;3(1):36-43.

Author

Uceda Otero, E. P. ; Eliel, G. S. N. ; Fonseca, E. J. S. ; Hickmann, J. M. ; Rodarte, R. ; Barreto, E.; Jalkanen, Karl J. / Study of cancer cell lines with Fourier transform infrared (FTIR)/vibrational absorption (VA) spectroscopy.

In: Current Physical Chemistry, Vol. 3, No. 1, 2013, p. 36-43.

Publication: Research - peer-reviewJournal article – Annual report year: 2013

Bibtex

@article{c88fd4e85ef949a3af55572eed825a5d,
title = "Study of cancer cell lines with Fourier transform infrared (FTIR)/vibrational absorption (VA) spectroscopy",
keywords = "Principal component analysis (PCA), Quadratic discriminant analysis (QDA), Linear discriminant analysis (LDA), Fourier transform infrared (FTIR), Vibrational absorption (VA), Cervical cancer, Bladder cancer, Henrietta Lacks (HeLa) cancer cell line",
publisher = "Bentham Science Publishers Ltd.",
author = "{Uceda Otero}, {E. P.} and Eliel, {G. S. N.} and Fonseca, {E. J. S.} and Hickmann, {J. M.} and R. Rodarte and E. Barreto and Jalkanen, {Karl J.}",
year = "2013",
volume = "3",
number = "1",
pages = "36--43",
journal = "Current Physical Chemistry",
issn = "1877-9468",

}

RIS

TY - JOUR

T1 - Study of cancer cell lines with Fourier transform infrared (FTIR)/vibrational absorption (VA) spectroscopy

A1 - Uceda Otero,E. P.

A1 - Eliel,G. S. N.

A1 - Fonseca,E. J. S.

A1 - Hickmann,J. M.

A1 - Rodarte,R.

A1 - Barreto,E.

A1 - Jalkanen,Karl J.

AU - Uceda Otero,E. P.

AU - Eliel,G. S. N.

AU - Fonseca,E. J. S.

AU - Hickmann,J. M.

AU - Rodarte,R.

AU - Barreto,E.

AU - Jalkanen,Karl J.

PB - Bentham Science Publishers Ltd.

PY - 2013

Y1 - 2013

N2 - In this work we have used Fourier transform infrared (FTIR) / vibrational absorption (VA) spectroscopy to study two cancer cell lines: the Henrietta Lacks (HeLa) human cervix carcinoma and 5637 human bladder carcinoma cell lines. Our goal is to experimentally investigate biochemical changes and differences in these cells lines utilizing FTIR spectroscopy. We have used the chemometrical and statistical method principal component analysis (PCA) to investigate the spectral differences. We have been able to identify certain bands in the spectra which are so-called biomarkers for two types of cell lines, three groups for the 5637 human bladder carcinoma cell line (5637A, 5637B and 5637C), and another one for the HeLa human cervix carcinoma cell line. The vibrational modes can be assigned to specific bands involving characteristic motions of the protein backbone. This work shows that infrared vibrational absorption (VA) spectroscopy can be used as a useful tool in medical diagnostics that provides in principle additional information and detail to that which can be obtained/provided from conventional histological studies, and more conventional mass spectroscopic and NMR techniques. The use of high level vibrational spectroscopic simulations, in addition to the chemometric and statistical tools of PCA, linear and quadratic discriminant analysis, and artificial networks methods that are good at finding correlations, but provide little if any physical, chemical and biochemical insight into the nature of the changes at a molecular level, is also strongly advocated and helpful to gain more physical, chemical and biological insight. Hence the combination of vibrational spectroscopic simulations and experimental vibrational absorption spectroscopy and imaging are advocated for future developments in this field.

AB - In this work we have used Fourier transform infrared (FTIR) / vibrational absorption (VA) spectroscopy to study two cancer cell lines: the Henrietta Lacks (HeLa) human cervix carcinoma and 5637 human bladder carcinoma cell lines. Our goal is to experimentally investigate biochemical changes and differences in these cells lines utilizing FTIR spectroscopy. We have used the chemometrical and statistical method principal component analysis (PCA) to investigate the spectral differences. We have been able to identify certain bands in the spectra which are so-called biomarkers for two types of cell lines, three groups for the 5637 human bladder carcinoma cell line (5637A, 5637B and 5637C), and another one for the HeLa human cervix carcinoma cell line. The vibrational modes can be assigned to specific bands involving characteristic motions of the protein backbone. This work shows that infrared vibrational absorption (VA) spectroscopy can be used as a useful tool in medical diagnostics that provides in principle additional information and detail to that which can be obtained/provided from conventional histological studies, and more conventional mass spectroscopic and NMR techniques. The use of high level vibrational spectroscopic simulations, in addition to the chemometric and statistical tools of PCA, linear and quadratic discriminant analysis, and artificial networks methods that are good at finding correlations, but provide little if any physical, chemical and biochemical insight into the nature of the changes at a molecular level, is also strongly advocated and helpful to gain more physical, chemical and biological insight. Hence the combination of vibrational spectroscopic simulations and experimental vibrational absorption spectroscopy and imaging are advocated for future developments in this field.

KW - Principal component analysis (PCA)

KW - Quadratic discriminant analysis (QDA)

KW - Linear discriminant analysis (LDA)

KW - Fourier transform infrared (FTIR)

KW - Vibrational absorption (VA)

KW - Cervical cancer

KW - Bladder cancer

KW - Henrietta Lacks (HeLa) cancer cell line

JO - Current Physical Chemistry

JF - Current Physical Chemistry

SN - 1877-9468

IS - 1

VL - 3

SP - 36

EP - 43

ER -