• Author: Willcocks, Margaret M.

    University of Surrey, Faculty of Health and Medical Sciences

  • Author: Locker, Nicolas

    University of Surrey, Faculty of Health and Medical Sciences

  • Author: Gomwalk, Zarmwa

    University of Surrey, Faculty of Health and Medical Sciences

  • Author: Royall, Elizabeth

    University of Surrey, Faculty of Health and Medical Sciences

  • Author: Bakhshesh, Mehran

    University of Surrey, Faculty of Health and Medical Sciences

  • Author: Belsham, Graham

    Sektion for Eksotiske Virussygdomme, Division of Virology, National Veterinary Institute, Technical University of Denmark, Lindholm, 4771, Kalvehave, Denmark

  • Author: Idamakanti, Neeraja

    Neotropix, Inc

  • Author: Burroughs, Kevin D.

    Neotropix, Inc

  • Author: Reddy, P. Seshidhar

    Neotropix, Inc

  • Author: Hallenbeck, Paul L.

    Neotropix, Inc

  • Author: Roberts, Lisa O.

    University of Surrey, Faculty of Health and Medical Sciences

View graph of relations

The RNA genome of Seneca Valley virus (SVV), a recently identified picornavirus, contains an internal ribosome entry site (IRES) element which has structural and functional similarity to that from classical swine fever virus (CSFV) and hepatitis C virus, members of the FLAVIVIRIDAE: The SVV IRES has an absolute requirement for the presence of a short region of virus-coding sequence to allow it to function either in cells or in rabbit reticulocyte lysate. The IRES activity does not require the translation initiation factor eIF4A or intact eIF4G. The predicted secondary structure indicates that the SVV IRES is more closely related to the CSFV IRES, including the presence of a bipartite IIId domain. Mutagenesis of the SVV IRES, coupled to functional assays, support the core elements of the IRES structure model, but surprisingly, deletion of the conserved IIId2 domain had no effect on IRES activity, including 40S and eIF3 binding. This is the first example of a picornavirus IRES that is most closely related to the CSFV IRES and suggests the possibility of multiple, independent recombination events between the genomes of the Picornaviridae and Flaviviridae to give rise to similar IRES elements.
Original languageEnglish
JournalJournal of Virology
Publication date2011
Volume85
Issue9
Pages4452-4461
ISSN0022-538X
DOIs
StatePublished
CitationsWeb of Science® Times Cited: 10
Download as:
Download as PDF
Select render style:
APAAuthorCBEHarvardMLAStandardVancouverShortLong
PDF
Download as HTML
Select render style:
APAAuthorCBEHarvardMLAStandardVancouverShortLong
HTML
Download as Word
Select render style:
APAAuthorCBEHarvardMLAStandardVancouverShortLong
Word

ID: 5528932