Simultaneous quantification of multiple bacterial metabolites using surface-enhanced Raman scattering

Research output: Research - peer-reviewJournal article – Annual report year: 2019

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Given the commercial importance of the compounds produced by genetically modified organisms, there is a need for screening methods which facilitate the evaluation of newly developed strains, especially during the phase of proof-of-concept development. We report a time-efficient analysis method for the screening of bacterial strains, which enables the detection of two structurally similar secondary bacterial metabolites. By combining liquid-liquid extraction and surface-enhanced Raman scattering we were able to quantify p-coumaric acid and cinnamic acid, produced by genetically modified E. coli from tyrosine and phenylalanine, respectively. With the simple sample pre-treatment method, and by applying a partial least squares data analysis method, we simultaneously detected the analytes from four E. coli strains cultured in the presence or absence of tyrosine and phenylalanine.
Original languageEnglish
JournalAnalyst
Number of pages12
ISSN0003-2654
DOIs
StateAccepted/In press - 2019
CitationsWeb of Science® Times Cited: No match on DOI
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